NCI-H2009[H2009]细胞系—人肺腺癌细胞系谁家更好 货号:GD-C16220400
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
MethylgerManiuM trichloride, 97%
8-BroMoquinoline
4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-HeptadecafluoroundecylaMine
2,5-DiMethyl-1,4-benzenediaMine
5,10,15,20-Tetraphenyl-21H,23H-porphine rutheniuM(II) carbonyl
1,2-Dichlorotetrafluoroethane
1,3,6,8-tetrabroMopyrene
2-Chloro-6-methoxytoluene
Indole-6-boronic acid
5-Oxocyclopent-1-ene-1-boronic acid 97%
Benzyl (2S,3R)-(+)-6-oxo-2,3-diphenyl-4-morpholinecarboxylate, 98%
4-BIPHENYLCARBOXAMIDE
Glycol dimercaptoacetate
tert-Butyl acrylate
L-Arginine Monohydrochloride
2-Acetyl-1-naphthol
2,3-DiMethoxyphenylboronic Acid (contains varying aMounts of Anhydride)
1-Boc-3-pyrrolidinecarbaldehyde
Zein, purified
St. Johns Wort Extract, 4:1, Powder
Behenic Acid
dibenzo[def,mno]chrysene-6,12-dione
6-bromoisatin
1-(2-Thiazolylazo)-2-naphthol
Trifluoromethoxybenzene
Pentafluorobenzyl chloride
(Z)-6-HENICOSEN-11-ONE
3-Benzyloxybenzaldehyde
2-NaphthylMagnesiuM broMide, 0.25M in MeTHF
(S)-1-Benzyl-3-[2-(Methylthio)ethyl], 97%
SaMariuM oxide
Hexabutyldistannane
OLEOYL CHLORIDE
OSMIUM
D-(+)-NEOPTERIN
ZINC ACETYLACETONATE HYDRATE
Ethyl 3,4-dihydroxybenzoate, 97%
N4-Benzoylcytosine
Phytyl Acetate (cis- and trans- mixture)
(R)-2-Chlorobutyric Acid
N-Benzyloxycarbonyl-L-asparagine
2-IODO-5-METHYLTHIOPHENE
(3-CHLOROPROPYL)TRIMETHYLSILANE
Nitrosobenzene
Tributyltin trifluoroMethanesulfonate
MonoMethyl Azelate
1-(5-Chloropyridin-2-yl)ethanone
3-Tolylboronic acid, 97%
2-(4-Methyl-1,3-thiazol-2-yl)acetonitrile, 97%
2,6-Dichlorophenol
4,4'-Difluorobenzhydrol
Undecanoic acid
S-(+)-3-Fluoropyrrolidine hydrochloride
Ethyl trans-3-(1-pyrrolidino)acrylate
trans-2-(Benzylamino)cyclohexanol
2-ETHYL-2-METHYL-1,3-DIOXOLANE
2-Hydroxy-4-iodobenzoic acid
9,9-Bis(4-hydroxyphenyl)fluorene
(S)-N-Boc-1,2,3,6-tetrahydro-2-pyridinecarboxylic acid
5-Amino-3-methyl-1-phenyl-1H-pyrazole-4-carbonitrile
4-(Chloromethyl)-2-phenyl-1,3-thiazole
4-Nitroaniline-2-sulfonic Acid Sodium Salt
3-Bromo-4-(trifluoromethoxy)benzyl alcohol 98%
ETHYL PENTAFLUOROPROPIONYLACETATE
2-Naphthyl benzoate
Dilthiazem hydrochloride
ACTINOMYCIND
4-Fluorophenyl acetate
1-broMo-3-ethylbenzene
1-Kestose
1-(3-BroMophenyl)
Tetraethyl orthocarbonate, 97%
Cresol Red
Guanylthiourea
rifamycin
Copper(I) sulfide
Amodiaquine HCL
2-Phenylpentane
aFGF ELISA Kit
S100A6 ELISA Kit
Human TPM1 ELISA Kit
UQCRC1 ELISA Kit
α-glucosidase ELISA Kit
Rat β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA Kit
CAV1 ELISA Kit
Human CCK ELISA Kit
Human plasma anticoagulant protein S,PS ELISA Kit
REG4 ELISA Kit
T1N0 Broth
Wheat Bran Medium
DIETHYL 2-ACETYLGLUTARATE
SaMariuM(III) fluoride, anhydrous, REacton|r, 99.9% (REO)
5,6,7,8-Tetrahydroquinoxaline
2-Fluoro-4-Iodobenzonitrile
dibenzo[def,mno]chrysene-6,12-dione
Dysprosium Fluoride
Silver sulfate
P-Hydroxybenzene propanoic acid
Monosodium fumarate
PODOPHYLLOTOXIN
N-Isopropyl-N-Phenyl-1,4-PhenylenediaMine
1-(2-Thiazolyl) hydrochloride, 97+%
2-Phenylanthraquinone, 98%
4′-(Octyloxy)-4-biphenylcarbonitrile
Phenylhydrazine sulfate
2-ForMylphenylboronic acid MIDA ester
N-Benzyl-N-Methyl 2-broMobenzaMide
2,3,4,5,6-PENTAFLUOROPHENYLACETONITRILE
Iron hydroxide oxide
D-Alaninol
5-Chloro-3-iodo-1H-indazole
4-Butoxyaniline
2-Bromo-5-methoxy-3-methylbenzoic acid