NCI-H1650细胞系—人肺支气管癌细胞系谁家更好 货号:GD-C16219040
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-n-Butyl-4'-n-propylbicyclohexyl, 97%
6-AMINO-3-BROMO-2-METHYLPYRIDINE
4-(EthoxyMethoxy)phenylboronic acid
COBALT STEARATE
(S)-(-)-4-tert-Butyloxazolidine-2,5-dione
4-(Morpholino)phenylboronic acid
ChloroMethane solution
1-(4-Methylphenyl)-1H-pyrrole-2-carbaldehyde, 97%
Ins(1,2,3,4,5,6)P6
2-(4-Methoxyphenyl)propan-2-amine
3-Acrylamidophenylboronic acid
2-Amino-3-chloro-5-nitrobenzonitrile
4-(Morpholin-4-yl)-piperidine dihydrochloride
Methyl phenyl sulfone
2-Isobutyl-3-Methoxypyrazine
TetraMethyl orthocarbonate
3,7-DiMethyl-1-octen-3-ol
N-Ethyl 3-broMo-5-MethylbenzenesulfonaMide
Triethylene glycol diMethyl ether, 99%, stabilized
PotassiuM Phosphate Tribasic, FCC
Reference Material of Sulfur in Residual Fuel Oil Certified by The Japan PetroleuM Institute, Sulfur Content 4Mass% level
2-Chlorobenzoyl chloride
6,7-dimethoxy-1H-isochromene-1,3(4H)-dione
2-Bromo-4-chlorobenzoic acid
Potassium thiocyanate
TRANS,TRANS,TRANS-1,5,9-CYCLODODECATRIENE
3-O-METHYL-ALPHA-D-GLUCOPYRANOSE
4,5,6-TrichloropyriMidine
3-Chloro-4-hydroxy-5-Methoxybenzyl alcohol, 98%
Methyl Methylphenylphosphinate, 98%
Hydrogen peroxide solution
IsobutylMagnesiuM BroMide
Tetrahydrofuran
POTASSIUM DIHYDROBIS(1-PYRAZOLYL)BORATE
Zinc pyrithione
3-Mercapto-2-pentanone, 98%
(2R,4R)-(-)-Pentanediol ,99%
Cyclododecyl isothiocyanate
2-(Chloromethyl)-4-nitrophenol
3-(Isopropylcarbamoyl)benzeneboronic acid 95%
2-PYRIDYLTHIOUREA
1,2,4,5-TETRABROMOBENZENE
2,6-Di-tert-butyl-4-methylphenol
Aluminum dihydrogen phosphate
6-AMino-5-nitro-3-picoline
Acetone ThioseMicarbazone
4-Methoxy-1h-pyrrolo[2,3-b]pyridine
3,5-Dichloroanthranilic acid, 97%
Methyl 2,6-dichloro-5-fluoronicotinate, 90%
Alendronate sodium
5-Norbornene-2-MethylaMine (Mixture of isoMers)
Diethyl phthalate
Carbamic acid, (2-azidoethyl)-, 1,1-dimethylethyl ester
1H-Imidazole, 1-hexadecyl-
3,4-Diamino-2-hydroxypyridine
N-(4-CARBETHOXYPHENYL)MALEAMIC ACID
METHYL QUINOLINE-6-CARBOXYLATE
3-Chloro-4-Methylacetanilide, 98%
2,5-Difluorobenzyl broMide
Bis(methylthio)trimethylsilylmethane
2-Piperidino-1,3-thiazole-5-carbaldehyde, 97%
2-Amino-4,5-dimethoxybenzonitrile
N-benzylbenzene-1,4-diamine(SALTDATA: FREE)
METHYL DECANOATE
N-Methyl-2,2,2-trifluoroacetamide
2,4-DIFLUORO-N-METHYLANILINE
Oxacillin SodiuM
alpha'-BroMo-alpha,alpha,alpha-trifluoro-M-xylene
1,5-Diphenyl-3-(4-Methoxyphenyl)forMazan
5-BroMobenzo[b]thiophene-2-boronic acid
5-BroMo-2-thiophenecarboxaldehyde, 97%
3-Methyl-2-furaldehyde, 97%
Atropine sulfate monohydrate
Tricosane
N-phenylphthalimide
4-(1,2-Benzothiazol-3-yl)-1-[(3aR,7aR)-octahydro-2H-isoindol-2-yl]piperazin-1-ium-1-sulfonate
Somatrem
4,4'-Bis(9-carbazolyl)-1,1'-biphenyl
CVL ELISA Kit
A-Glu ELISA Kit
Human MUC3 ELISA Kit
Human Chlamydia pneumoniae antibody,Cpn-Ab ELISA Kit
PGC-1β ELISA Kit
S-100 ELISA Kit
Chicken Interleukin 2,IL-2 ELISA Kit
Human GH (Growth Hormone) ELISA Kit
Human VEGFA ELISA Kit
NN-T4 ELISA Kit
Anaerobe Broth MIC
Soybean sprout Extract Sucrose Agar Medium
4-Methyl-3-Oxovaleric Acid Methyl Ester
N,N-DiMethylthiourea
5-Chlorobenzo-2,1,3-thiadiazole, 98%
2-Fluoroanisole, 98%
2-Chlorobenzoyl chloride
Methylhesperidine
Calcium hypophosphite
Chlorodifluoroacetophenone
1H,1H-PENTADECAFLUORO-1-OCTANOL
3-Methylthiophene
L(-)-Tryptophan
Ethyl 3-(4-Morpholinyl)propionate, 97%
Selenourea, 99%
Glycidyl Methacrylate
Di-tert-butylMethylphosphine
Diethyl (3-Chlorobenzyl)phosphonate , 95%
4-AMino-3-hydrazino-1,2,4-triazol-5-thiol
Hexahydro-4-Methylphthalic anhydride
FMoc-3-cyano-D-phenylalanine
Chloromethyl-4-chlorophenyl dimethylsilane, 97%
2-Bromo-3-fluoro-6-methylpyridine
3-(chloromethyl)-5-cyclopentyl-1,2,4-oxadiazole(SALTDATA: FREE)
Sulfonyl Chloride Polystyrene Resin cross-linked with 1% DVB (50-100mesh) (4.5-4.8mmol/g)