Mv1Lu细胞系—貂肺上皮细胞系哪家专业 货号:GD-C16120544
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
PotassiuM phenoxyMethyltrifluoroborate, 95%
Methyl p-toluate
VitaMin K1
2,3-DICHLOROISOBUTYRIC ACID
4-(1-BOC-piperidin-4-yloxy)-2-Methoxyphenylboronic acid
INDOLO(2,3-B)CYCLOHEPTENE
(S)-1-[2-(Diphenylphosphino)phenyl]ethylamine, min. 97%
3-Chloropivalic Acid
Cerium(III) phosphate hydrate
Olive oil, virgin, pure
Polyvinylpyrrolidone, cross-linked
3-AMINO-2-FLUORO-4-PICOLINE
1,2-DICHLORO-1-FLUOROETHYLENE
p-Phenylenediamine
EthylhexadecyldiMethylaMMoniuM BroMide
1,3-Benzodioxole-4-carbaldehyde
4,4'-DibroMo-2,2'-bithiophene
1-(Pyrrolidin-1-ylcarbonyl)-2-broMobenzene
4-Methyl-1,2,3-thiadiazole-5-carbonyl chloride, 90%
TripelennaMine Hydrochloride, USP
Theophyllol
sodium ethanethiolate
amino(1H-indol-3-yl)acetic acid
Sulphur Yellow 9
n-Amylbenzene
2-Amino-3,5-dichloropyridine
3,3',4,4'-TETRACHLORODIPHENYL SULFONE
1-Heptyne
1,1,2,2-Tetraallyloxyethane, tech. 80%
N-Acetyl-D-norvaline, 98%
3-Fluoro-4-nitroaniline
2,5-DiMethylpyrrole
1,5-Bis(diphenylphosphino)pentane
N-Methyl 4,5-dibroMo-2-Methoxyaniline
2-Chlorobiphenyl-3-carboxylic acid
CYCLOHEXYL VALERATE
Methyl Phosphorodichloridate
1-(4-Chlorophenyl)-1-cyclopentanecarboxylic acid, 98%
Crystal Violet, indicator, pure
2-Amino-5-chlorobenzenesulphonic acid
2-Bromoadamantane
Hexamethyldisilane
ATROPINE METHYL NITRATE
Beta Alanine
DiMethylphenylphosphine
2-Fluoroisonicotinic Acid
Picrolonic Acid
5-(4-Methylphenyl)pyridin-2-aMine
2-Chloro-5-(trifluoroMethyl)benzene-1-sulfonyl chloride, 97%
H-ILE-BETANA
2,4-DiMethylquinoline
4-Bromo-3-(trifluoromethyl)benzaldehyde
3-Aminocyclopentanecarboxylic acid
Ethyl 4-hydroxy-3-nitrobenzoate
D(-)-lactic acid
Bis(1,1-dimethylpropyl) peroxide
2-(3-BroMophenyl)-2-Methylpropanenitrile
2-Vinylpyridine
2,2'-DIHYDROXYDIPHENYL ETHER
ETHYLENE GLYCOL DI-N-BUTYL ETHER
2-[(4-Chlorophenyl)thio]aniline
Enoxacin Sesquihydrate
3-Chloro-4-nitrobenzotrifluoride
1-(1-benzyl-1H-pyrazol-4-yl)methanamine(SALTDATA: FREE)
Bis(2-dimethylaminoethyl) ether
Triadimenol A
4-(Trifluoromethoxy)phenyl isocyanate
5-Phenyl-1H-tetrazole
1-Hexanethiol
2,5-Diacetoxyhexane
N-Isobutyl 2-broMo-5-fluorobenzaMide
TriMethylsilyl broMoacetate, 98%
ProtaMine Sulfate, USP
3,5-Bis[3,5-bis(3,5-diMethoxybenzyloxy)benzyloxy]benzyl BroMide
2,4-Dibromobenzoic Acid
2,5,6-trimethylbenzothiazole
p-Toluenesulfonyl azide
2,2'-bis(trifluoromethyl)benzidine
NT-1R ELISA Kit
Mouse PICP (Procollagen I C-terminal Propeptide) ELISA Kit
SOST ELISA Kit
Human Peptide YY ELISA Kit
FDP ELISA Kit
gGT1 ELISA Kit
ANG-Ⅱ ELISA Kit
Human PKCη (Protein Kinase C Eta ) ELISA Kit
Human PA-IgM ELISA Kit
TNF ELISA Kit
Brilliant Green Lactose Bile Broth
Lactose Bile Broth
1-Hydroxyethylidenebis(phosphonic acid), 96%
Phenothiazine
4-n-Pentyloxybenzeneboronic acid, 96%
2-Iodo-5-(trifluoroMethyl)pyridine, 96%
sodium ethanethiolate
prostaglandin A(1)
Acid Red 226
Maleimide
2-Amino-5-bromobenzotrifluoride
4-Methylbenzyl chloride
Di-Tert-Butyl IMinodicarboxylate
Ethyl 4-Methyl-2-[4-(trifluoroMethyl)phenyl]thiazole-5-carboxylate, 97%
10-(2-Naphthyl)anthracene-9-boronic acid, 97%
Ethyl nicotinate
trans-2-Hexen-1-ol
3,5-DiMethyladaMantane-1-acetic acid
4-(4-Isopentyloxyphenyl)phenylboronic acid
3-Phenylaziridine-2-carboxaldehyde diMer
ERBIUM CHLORIDE
Pent-4-ynoic acid
1,1'-Bis(di-i-propylphosphino)ferrocene(1,5-cyclooctadiene)rhodium(I) tetrafluoroborate, min. 98%
Methyl 3,6-di-O-(α-D-mannopyranosyl)-α-D-mannopyranoside ,98%
1-N-Boc-3-carbamoylpiperidine