Mv 1 Lu(NBL-7)细胞系—貂肺上皮细胞系哪家专业 货号:GD-C16120444
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Oxalic dihydrazide, 98%
Fenbuconazol
Tetraiodoethylene
ALPHA-D-GLUCOSE-1-PHOSPHATE NA2-SALT
2,7-DibroMofluorene
RubidiuM chloride
SODIUM METABORATE TETRAHYDRATE
2,4-Di-tert-butylphenol, 97%
Di-2-ethylhexylamine, 99%
N-Methyl-3-(trifluoromethyl)aniline
Diphenylammonium Trifluoromethanesulfonate
Carbazole-9-ethanol
3',4',7-Trihydroxyisoflavone
1,2,3,4-Tetrahydro-1-naphthol
5-BroMo-1-benzothiophene
Dicyclopentadiene (stabilized with BHT)
1,4-DibroMo-2,5-bis(broMoMethyl)benzene
Methyl 2-broMo-6-Methylbenzoate
(E)-3-Acetoxy-1-propenylboronic acid pinacol ester, 97%
SuMatriptan Succinate, EP
Methyl 4-(AMinoMethyl)benzoate Hydrochloride
2,3,5,6-Tetrafluoroaniline
Pigment Violet 23
cholesteryl N-decylate crystalline
1-Chloro-2-fluoro-4-methoxybenzene
CYCLOHEXYL ISOTHIOCYANATE
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
2-AMino-6-Chloropyridine
n-Dodecyltriethoxysilane, 95%
1-AMino-4-cyclopentyl, 97%
ZirconiuM carbide
cis-10-Heptadecenoic acid
3-BroMo-2-butoxy-5-fluorophenylboronic acid
N-Z-4-pentyne-1-aMine
6-broMo-4,4-diMethyl-3,4-dihydroquinolin-2(1H)-one
Di-μ-chlorodiMethylbis(triphenylphosphine)dipalladiuM
5-(Chloromethyl)-2-(1H-pyrazol-1-yl)pyridine ,97%
(4-fluorobenzyl)propylamine(SALTDATA: HCl)
5-Bromo-5-deoxy-2,3-O-isopropylidene-D-ribono-1,4-lactone
2-Mercaptopyridine-N-oxide, sodium salt monohydrate, 98%
Acid Blue 83
2,3,6-Trifluorophenylboronic acid
2-Formylcinnamic acid
1-PENTANESULFONIC ACID SODIUM SALT MONOHYDRATE
4-BroMo-2,5-difluorobenzenesulfonyl chloride
PhosphoMolybdic acid hydrate
ThiaMine Disulfide Nitrate
2,2'-DibroMobiphenyl, 98%
4-BroMo-2-chlorotoluene, 98%
1,1'-Oxydi-2-propanol
3,3-Difluoropyrrolidine Hydrochloride
tris(2,6-dimethoxyphenyl)phosphine
2'-O-t-Butyldimethylsilyl-5'-O-(4,4'-dimethoxytrityl)-N4-benzyl cytidine
N-Trimethoxysilylpropyl-N,N,N-trimethylammonium chloride
3,5-Dimethoxybenzonitrile
4-Amino-2,6-dichloropyrimidine
2-AMino-5-Methylthiophene-3-carbonitrile
(3S,4R)-4-(4-Fluorophenyl)-3-hydroxyMethyl-1-Methylpiperidine
AMMoniuM cupric chloride dihydrate
cis-4-Octene
4-Nitrophenyl 2-Acetamido-2-deoxy-beta-D-glucopyranoside
1-(4-(2-Aminoethyl)piperazin-1-yl)ethanone
2-(2-Benzothiazolyl)-3,5-diphenyltetrazolium Bromide
4-N-Boc-aminocyclohexanone
5-Chloro-2-nitrobenzotrifluoride
Allyl ether
1,3-BIS(ISOCYANATOMETHYL)CYCLOHEXANE
Isobutyl Mercaptan
1-Butyl-2-pyrrolidone
Ethyl 5-cyclopropyl-2H-pyrazole-3-carboxylate
Acridine hydrochloride, 97%
5-BroMo-1-Methyl-1H-indole-3-carbaldehyde, 97%
2-Phenylpropylene Oxide
4-(1-Naphthylvinyl)pyridine
alpha-Angelica lactone
9H-fluoren-9-ylmethyl piperidin-4-ylcarbamate hydrochloride
2-(5-Methylthiophen-2-yl)glycine
Trisilane, 1,1,1,3,3,3-hexachloro-2-(trichlorosilyl)-
Porcine Leptin,LEP ELISA Kit
Mouse P-Selectin ELISA Kit
Human PCDH11 ELISA Kit
Human DPP4 ELISA Kit
5HT/ST ELISA Kit
Rat β-Defensins,β-DF ELISA Kit
Chicken Immune RNA,Irna ELISA Kit
Human PC ELISA Kit
Human SAP ELISA Kit
HP-IgY ELISA Kit
Double-arginine Hydrolase Test Medium
Casein Digest Soybean Agar for Pzase Test
Lead(II) 2,4-pentanedionate
Potassium fluoride dihydrate
5-(Tri-n-butylstannyl)pyriMidine, 96%
2-Furaldehyde, 98%
2,3,5,6-Tetrafluoroaniline
1,1-Dimethylthio-2-nitroethylene
Tellurium dioxide
Hexadecanedioic acid
2,2'-Dithiosalicylic acid
MUCIC ACID
Iron(Iii) Sulfate N-Hydrate
Methyl 3-broMo-2-fluorobenzoate, 98%
6-AMinocouMarin hydrochloride, 97%
Boc-L-Lys(FMoc)-OH
2'-Deoxyuridine
Ethyl (4-trifluoroMethylbenzoyl)acetate
1-BROMO-3,4-DICHLOROBUTANE
Methyl 3,4-diMethylbenzoate
4-N-OCTYLANILINE
3-methyl-1-phenyl(SALTDATA: HCl)
Tetraoctadecyloxysilane
D-2-Allylglycine Hydrochloride
Tetramethyl 1,1,2,3-Propanetetracarboxylate