Neuro-2a细胞系—小鼠脑神经瘤细胞系哪家专业 货号:GD-C16119301
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Isopropylaniline, 99%
Methyl acetate
TrifluoroacetaMidine
2-Chloro-6,8-diMethylquinoline
1,1′-Bis((2R,5R)-2,5-diisopropylphospholano)ferrocene(cyclooctadiene)rhodiuM(I) tetrafluoroborate
Manganese bis(2-ethylhexanoate)
2-Butyl-1-octanol
2-(Trifluoromethyl)propenoic acid
2-Methoxypyridine-3,4-diamine
3-Amino-2-fluorobenzoic acid
N,N,N',N'-Tetramethyl-2,2-dimethyl-1,3-propanediamine
4-Pyrid-4-ylbenzoic acid
Ethyldichlorosilane
2,2'-Thiobisethylamine
1-(1-Benzothiophen-5-yl)-2-broMo-1-ethanone
EthylaMine hydrochloride
2,4-DiMethoxy-1,3,5-triazine
2-BroMo-4-iodobenzaldehyde
(R)-(+)-N-BOC-2-Piperidinecarboxylic acid, 98%
Testosterone, Topical (CIII), Micronized, USP
DL-Alanylglycylglycine Dihydrate
tranexamic acid
2,6-Dimethoxy-4-methylphenol
Diaminomaleonitrile
Cyclopentadecanone
4-ALLYL-1,6-HEPTADIEN-4-OL
Sudan Red 7B
Methyl 4-broMocrotonate, tech. 85%
Ethyl (Z)-3-cyano-^a-MethylcinnaMate
1-(4-Fluoro-2-nitrophenyl)pyrrolidine, 97%
BROMOCHLOROACETIC ACID
2-CHLORO-5-(TRIFLUOROMETHYL)PHENYL ISOTHIOCYANATE
N-SucciniMidyl 3-MaleiMidopropionate
4-HYDROXY-1-PHENYLPYRAZOLO[3,4-D]PYRIMIDINE
4-BroMo-2,8-diMethylquinoline
boc-Glutaminol
N-Methylethylamine 97%
3-(Bromomethyl)piperidine, N-BOC protected
2-Amino-4-carboxybenzeneboronic acid hydrochloride 96%
2-Phenylacetamide
2-Aminothiazole-4-carboxylic acid
Phosphorus
3-[(2-Chlorophenyl)amino]propanenitrile
2H-Pyran-2-one
Cholesterol Ethyl Carbonate
5-Methoxy-1H-indole-2-boronic acid pinacol ester
Ethyl 9H-fluorene-9-acetate, 98%
5-(TrifluoroMethyl)nicotinonitrile, 95+%
1,6-DibroMododecafluorohexane
6-HydroxycouMarin
2,3,5-TRICHLORO-4-PYRIDINOL
Methanesulfonic acid
Capecitabine
(2S)-2-amino-3-phenyl-1-(piperidin-1-yl)propan-1-one
(R)-2-Aminohexane
(S)-N-BOC-2-BroMophenylalanine
BROMOMETHYL 2-NAPHTHYL KETONE
ISOPROPYL PROPIONATE
4-Phenyl-1H-pyrazol-5-amine
Bis(2-diphenylphosphinoethyl)phenylphosphine, 97% TRIPHOS
2,2,2-Trifluoroethyl vinyl ether 97%
2-Phenyl-1,3-dioxan-5-ol
(1R,2S)-1-Amino-2-indanol
Bis(trimethylsilyl)methane
Feprazone
1-Chlorododecane
2,2,2-Trichloroethanol
1-Butyl-4-nitrobenzene
3,5-Dichlorophenylboronic acid, pinacol ester
Copper(I) thiophene-2-carboxylate, 90%, May contain approx. 20 wt.% Copper(I) oxide
Methyl 3-(trifluoroMethoxy)benzoate, 98%
2-AMino-3-chloropyrazine
4-MethoxycinnaMonitrile (cis- and trans- Mixture)
chromium acetate
1-(2-chloroethyl)-4-octyl-Benzene
Danofloxacin mesylate
4-tert-Butyl-3-methoxybenzoic acid
RB1 ELISA Kit
PSPN ELISA Kit
Human iNOS ELISA Kit
DPYSL-2 ELISA Kit
TNF-β ELISA Kit
β-END(BEP) ELISA Kit
BDNF ELISA Kit
Human POFUT2 ELISA Kit
Human Thromboxane A2,TX-A2 ELISA Kit
FBN1 ELISA Kit
Gelatin Medium
Mannitol Fermentation Medium
HolMiuM(III) 2,4-pentanedionate, REacton|r, 99.9% (REO)
Thulium trifluoride
5(6)-Carboxyfluorescein, Mixture of isoMers, 97%
Allyl crotonate, 98%
tranexamic acid
3-BROMO-4-CHLOROPHENOL
Lithium niobate
Iodosylbenzene
Phenol Red sodium salt
TITANIUM
1,2,4-Triazolo[4,3-a]Pyridin-3(2H)-One
4-Chloro-2-(trifluoroMethyl)quinoline, 98%
Methyl 3-thiopheneacetate, 98%
Allyl iodide
trans,trans-2,4-Nonadienal
4-Chloro-3-Methoxy-2-Methylpyridine N-oxide
3',5'-DiMethoxyacetophenone
9,10-DIHYDROANTHRACENE
2,2'-DICHLOROBENZILIC ACID
3-(tert-Butoxymethyl)phenylboronic acid
1,4-Dioxane-2,5-dione
9-Aminoacridine Hydrochloride Monohydrate
Cinchonidine Dihydrochloride