RPTEC细胞系*人肾近端小管上皮细胞系开学季大放送 货号:GD-C15120556
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 1H-indazole-7-carboxylate, 95%
α-AMinoisobutyric acid
4 5-DIHYDROXY-1 3-BENZENEDISULFONIC ACI&
3,4-Methylenedioxyphenylboronic acid
1-(4-Chlorophenyl)cyclopropanaMine, HCl
2-Methyl-3,5-dinitrobenzyl alcohol
Tris(2,4-pentanedionato)chromium(III)
Boc-2-chloro-D-phenylalanine
5-Bromo-3-fluoropyridine-2-carboxylic acid
4,6-Dimethylpyrimidine
1-(3-Bromopropoxy)-3-methylbenzene, 95%
HEPTANOPHENONE
1-BENZOYL-3-(2-PYRIDYL)-2-THIOUREA
4-Hydroxy-4-methyl-2-pentanone
2-Pyridin-3-ylbenzaldehyde
Oxalic acid bis(cyclohexylidenehydrazide)
3,4-Dihexylthiophene
tert-Butyl 3-broMoindazole-1-carboxylate
Citraconic anhydride, 98%
Tyrphostin 23
DL-3-(3,4-Dihydroxyphenyl)alanine
Diethyl chlorophosphate
trihydroxyethylrutin
Zeolite
Ethyl 3-bromopropionate
2,5-Dimethoxy-4-chloroaniline
TRIMETHYLENE BORATE
Pyrazinecarbonitrile
N-Boc-N-Methyl-L-alanine, 98%
N-Boc-S-(4-Methoxybenzyl)-D-cysteine, 95%
DiMethyl oxalate
2-BroMo-9,9'-spirobi[9H-fluorene]
1-(5-Methyl-2-thienyl)ethan-1-one
Nitroxylol
(2-BENZIMIDAZOLYLTHIO)ACETIC ACID HYDRAZIDE
5-(3,5-Dichlorophenyl)-1H-tetrazole, 97%
7-chloro-6-methyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid(SALTDATA: FREE)
4-Hydrazinobenzoic acid, 98%
Ginsenoside Rf ,98%
3-Chloro-5-(chlorosulfonyl)benzoic acid
3-BUTYLPYRIDINE
DIETHYL 4-AMINOBENZYLPHOSPHONATE
2-(Trifluoromethoxy)terephthalic acid
Dinonyl o-phthalate;DNP
Tetrabutyl orthosilicate
Hexythiazox
(Perfluoro-n-octyl)phenyliodoniuM TrifluoroMethanesulfonate
(2-BroMoethyl)triMethylaMMoniuM broMide, 98%
6-Chloro-2,4-diMethoxypyriMidine, 98+%
3,4-DEHYDRO-DL-PROLINE
Methyl 3-AMino-4-chlorobenzoate
2-Amino-5-bromo-6-picoline
2-(trimethylsiloxy)furan
(2-Dimethylaminoethyl)triphenylphosphoniumbromide
Dryocrassin
2-Chloropyridine-N-oxide
1-ISOPROPYL-3-PYRROLIDINOL
1-(2-Methoxyphenyl)ethanol
2-Hydroxy-4-Methylpyridine
4-[2-Amino-4-(trifluoromethyl)phenoxy]benzonitrile, 97%
2-[2-(Furan-2-yl)vinyl]-4,6-bis(trichloromethyl)-1,3,5-triazine
p-Xylylene Dithiocyanate
o-Tolyl 3,5-Xylyl Ether
1,3-Dibromo-2,2-dimethoxypropane
2-ETHYLIODOBENZENE
2,3-Dichlorophenol
D-GlutaMic acid;D-2-AMinopentanedioic acid;D-α-AMinoglutaric acid;D-1-AMinopropane-1,3-dicarboxylic acid;(R)-2-AMinoglutaric acid
MaleiMide
1-Hydroxy-4-p-toluidinoanthraquinone
O-Phospho-L-tyrosine
(+)-Diethyl L-tartrate, 99+%
2-ThiophenecarboxiMidaMide hydrochloride, 95%
L(+)-2-Aminobutyric acid
N-(3-AMinopropyl)-2-nitrobenzenesulfonaMide Hydrochloride
Methylphenyl
citalopram
2-Nitroisophthalic acid
Copper(II) carbonate basic
NSE ELISA Kit
COL-1 ELISA Kit
IDO ELISA Kit
Human GALNT13 ELISA Kit
Monkey cTn-I/TNNI3 (cardiac Troponin I) ELISA Kit
ADR ELISA Kit
ANGPT1 ELISA Kit
PKK9 ELISA Kit
Human platelet membrane glycoprotein Ⅳ,GP-Ⅳ ELISA Kit
TRAIL ELISA Kit
Double Lactose Bile Medium Base
Desoxycholate Lactose Agar
Silver acetate
Molecular sieves, 4A, 0.4-0.8MM (0.02-0.03in) beads
4-n-Pentylbenzenesulfonyl chloride, 97%
2-Iodo-1-MethyliMidazole, 97%
Diethyl chlorophosphate
magnesium sulfate monohydrate
1,1'-Ferrocenedicarbonyl dichloride
1,2-Dimethylnaphthalene
ALUMINUM DI(ISOPROPOXIDE)ACETOACETIC ESTER CHELATE
MAGNESIUM ION CHROMATOGRAPHY STANDARD
Citronellyl Propionate
2-Boc-2-azabicyclo[2.2.1]hept-5-ene, 98%
Filter Aid, Celite AW Standard Supercel|r
Methyl 5-ForMylsalicylate
2-Chloro-1,3-diMethyliMidazolidiniuM hexafluorophosphate
2-BroMoquinoline
4-(4-Fluorobenzoyl)butyric acid
1,2-DibroMo-4,5-difluorobenzene
(R)-5-(BroMoMethyl)-2-pyrrolidinone
8-Methyl-4-quinolinol
6-Methoxyflavanone, 98%
N-(4-Fluorophenyl)formamide
H-D-Asp(OtBu)-OH