H4-ⅡE细胞系*大鼠肝癌细胞系开学季大放送 货号:GD-C15120517
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-BroMo-3-(trifluoroMethoxy)phenol, 97%
ZirconiuM(IV) oxide-yttria stabilized
2-Fluorophenylacetone
4-Chloro-3-cyanophenylboronic acid
2,6-DiMethylpyridine-4-boronic acid, pinacol ester
2,3,5-TRIPHENYLTETRAZOLIUM IODIDE
Cresyl Violet acetate, high purity biological stain, pure
2-Fluoro-5-(hydroxymethyl)benzonitrile
Bis(tetrahydro-2H-pyran-4-yl)amine 97%
(2-phenyl-2-piperidin-1-ylethyl)amine(SALTDATA: 2HCl)
N-Butylpropionamide
2-BUTYL-2-ETHYL-1,3-PROPANEDIOL
4-FLUORO-3-(TRIFLUOROMETHYL)BENZOYL CHLORIDE
2-Bromo-3-fluoronitrobenzene
3-Nitrobenzoic acid
Bis(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate
9,10-Di(2-naphthyl)anthracene
3-BroMo-5-(trifluoroMethoxy)benzaldehyde
5-(2-Chlorophenyl)furfural
TOLUENE
N-PhenylMaleiMide
2,4-Dinitroiodobenzene
2-Pyrazinylmethanol
3-Methoxy-1-propanol
4-Ethylpyridine
4-Butylaniline
3-Hydroxybenzaldehyde
(S)-(-)-1-(1-Naphthyl)ethylaMine, ChiPros 99+%, ee 99+%
4-BroMo-3-chlorobenzoic acid, 97%
Ethyl trans-2-decenoate, 97%
N,N-DiethylethylenediaMine
3-PHENOXYANILINE
Ethyl adaMantane-1-carboxylate
N-MethylhoMo
tert-Butyl 2-broMo-5-Methoxybenzoate
EDTA ferric sodiuM salt
5-Amino-2-fluoro-4-methylpyridine
5-Fluorouridine 97%
1,2-Dibromo-1,1-difluoroethane, 98%
1,3,6,8-Tetrabromopyrene
2-BROMOPHENYL ISOTHIOCYANATE
R-Tetrahydropapaverine
2,5-Dimethyl-2,4-hexadiene
4-Acetylbenzenesulfonyl chloride
SodiuM Methoxide
1,3-Dibenzyloxybenzene
5-Chloro-6-isopropoxypyridine-3-boronic acid
Bis(triphenylphosphine)palladiuM(II) acetate, 99%
2-BroMophenoxyacetonitrile, 99%
Ethyl Heptadecafluorononanoate
Cholesterol Nonyl Carbonate
9,10-Methylene-9-octadecenoicacid
5-(hydroxymethyl)pyrazine-2-carboxamide
Indeloxazine hydrochloride
Poly(2,6-Dibromophenylene Oxide)(PBO)
8-Hydroxyquinoline-N-oxide
5-CHLORO-2-ADAMANTANONE
2-(METHOXYCARBONYL)PHENYL ISOCYANATE
3-BroMo-2-hydroxybenzonitrile
SodiuM 2-(7-(3,3-diMethyl-1-(4-sulfonatobutyl)benz(e)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-diMethyl-1-(4-sulfonatobutyl)benz[e]indoliniuM
1-(4-Chlorophenyl)-3-phenyl-2-thiourea
1,2-Bis((2S,5S)-2,5-diisopropylphospholano)benzene(cyclooctadiene)rhodium(I) tetrafluoroborate, 97%
α-D-Glucopyranose pentabenzoate ,98%
Acetyl Sulfide
3-Nitrobenzyl bromide
2-Ethyl anthraquinone
BOC-D-TYR(BZL)-OH
Pararosaniline base
1-Ethyl-3-MethyliMidazoliuM chloride
N,N'-DiisopropylethylenediaMine
Methyl 2-broMo-5-isopropylthiazole-4-carboxylate
Propiophenone, 99%
Sulfadiazine, Powder, USP
n-OctylaMine
1-Iodo-3-nitrobenzene
N-Benzoylsuccinimide
(+)-trans-Tetramethrin
carvacrol
GDF5 ELISA Kit
SELL ELISA Kit
FCHOL ELISA Kit
CA ELISA Kit
Monkey ADP/Acrp30 (Adiponectin) ELISA Kit
GABA ELISA Kit
AREG ELISA Kit
Human PICK1 ELISA Kit
Human ADAMTS1 ELISA Kit
Lp-PL-A2 ELISA Kit
Modified Transport Medium
Slantz and Bartley medium
Vinyltriethoxysilane
(Z)-2-MethoxyiMino-2-(Fur-2-yl)-Aceticacid ,AMMoniuM Salt
1,5-EDANS
2'-Iodoacetophenone, 98+%
2,4-Dinitroiodobenzene
DL-o-Chlorophenylalanine
Acid Orange 88
2-Bromoethanol
2-Amino-4-hydroxy-1H-pteridine
4-N-PENTYLBIPHENYL
2-AMino-1,3-Propanediol
PotassiuM tetranitropalladate(II), Pd 28.3% Min
4-(TrifluoroMethoxy)phenylacetonitrile, 98%
Diethyl acetylMalonate
4'-DiMethylaMinoacetophenone
4-AMINO-1,3,5-TRIMETHYLPYRAZOLE
CIS-EPOXYSUCCINIC ACID
2-Cycloheptenone
Cyclohexyl chloroforMate
3,4,5-Trifluorophenol
D(-)-Ribose, 99+%
9-Ethynyl-9-fluorenol
3-Hydroxy-cyclobutanecarboxylic acid ethyl ester