HTR-8细胞,人滋养细胞系多久换新培养基 货号:GD-C14619645
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(1R,2S)-1-Phenyl-2-(1-pyrrolidinyl)propan-1-ol
2,4,5-Trichloroaniline
LEAD (II) CYANURATE
4-BroMo-2-Methoxybenzonitrile
5-Chloro-1-aza-5-stanna-bicyclo[3.3.3]undecane
2,4,6-Triallyloxy-1,3,5-triazi
(S)-1-(Pyridin-2-yl)ethanol ,98%
1,2,3-Cyclohexanetriol (cis- and trans- mixture)
2-(isopropylthio)aniline(SALTDATA: HCl)
1H-Indole, N-BOC protected 98%
(R)-N-Boc--2-carboxylic acid methyl ester
4,5-DICARBOXY-1-METHYL-1H-IMIDAZOLE
4-HYDROXY-4'-IODOBIPHENYL
Ethoxyamine hydrochloride
PotassiuM persulfate
tert-Butyl 2-broMobenzylcarbaMate
4,4'-Ethylidenebisphenol
3'-Chlorobiphenyl-3-carboxylic acid
L-3,4-Dihydroxyphenylalanine Methyl ester hydrochloride, 95%
CARBAZOLE
EthylenediaMinetetraacetic Acid DisodiuM Nickel(II) Salt Hydrate
4-Bromobenzaldehyde
Ammonium phosphate
2-chloroadenine
2-(β-D-glucopyranosyloxy)benzaldehyde
1-Aminocyclopropanecarboxylic acid
ENDOSULFAN LACTONE
[1-(Ethoxycarbonyl)Ethyl]TriphenylphosphoniuM BroMide
Bis(carboxyethylgerManiuM) sesquioxide, 99.7%
Borane-diphenylphosphine coMplex, 98%
Boron
4-(1-Pyrrolidino)benzaldehyde
2-(4-CHLOROPHENYL)BENZOTHIAZOLE
N,N'-DiMethyl-1,2-cyclohexanediaMine
2,3-DiMercapto-1-propanol
9-Phenyl-9-fluorenol
Ethyl 4-hydroxy-6-(trifluoromethoxy)quinoline-3-carboxylate
4-(6-Chloropyrimidin-4-yl)morpholine
N-(6-Chloro-4-pyrimidinyl)-N,N-diethylamine
4-Methoxy-2-nitrobenzoic acid
Ammonium citrate dibasic
D-Tryptophanol
Ethyl nitrate
PHTHALIC ACID MONOBENZYL ESTER
1,1'-Bis(diphenylphosphino)ferrocene-palladiuM(II)dichloride dichloroMethane adduct
Poly(N-vinylcarbazole)
1,4-Epoxy-1,4-dihydronaphthalene
2-AcetaMido-1-nitro-3,5,6-trifluorobenzene
2-AMino-4,5-diMethoxybenzoic acid, 98%
DICLOFLUANID
2,6-Bis(chloroMethyl)pyridine
Furfuryl methyl sulfide
Diastase
2,4,5-Tribromoimidazole
Benzyl sulfone; Dibenzyl sulphone;
TRIBUTYL(3-METHYL-2-BUTENYL)TIN
METHYL ISOCYANOACETATE
1-BroMo-3-isobutylthiobenzene
Adenosine-5'-diphosphate disodiuM salt
1,3,5-Tris(4-bromophenyl)benzene
4-Methoxy-2-methylbenzoic acid, 97%
5-methyl-2-piperidin-4-yl-1H-benzimidazole(SALTDATA: FREE)
Amyl Decanoate
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
6-(Trifluoromethyl)indole
4-Hydroxy-3-nitropyridine
Dimethyl trisulfide
N-Ethyl-N-hydroxyethylaniline
2,2-Bis(2-hydroxy-5-biphenylyl)propane
5-Cyano-2-hydroxyphenylboronic acid
Chlorogenic acid, 98%, predoMinantly trans, froM coffee seeds
2,2,2-Trifluoro-1-phenyl-ethylaMine hydrochloride, 95%
Meso-1,2:3,4-Diepoxybutane
Ethyl 4-BroMophenylacetate
2-Cyclohexen-1-one
Phosphine,butylbis(tricyclo[3.3.1.13,7]dec-1-yl)-, hydriodide (1:1)
7-Bromoquinazoline-2,4(1H,3H)-dione
Metadoxine
FⅤ ELISA Kit
BAFFR ELISA Kit
Human IAPP ELISA Kit
Human RETNLβ ELISA Kit
MHC III/AgB I/H-1 III ELISA Kit
IL6R ELISA Kit
IFNα ELISA Kit
Human LOR ELISA Kit
Human PANCPIN ELISA Kit
Mouse C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit
Diamond’s TrypticaseYeast Extract Maltose Complete
BAT Broth
Ethyl 4-Methyl-2-(2-thienyl)thiazole-5-carboxylate, 97%
trans-4-n-Hexylcyclohexanecarboxylic acid, 99%
4-AcetaMidobenzenesulfonyl Azide
2-AMinooxazole, 97%
4-Bromobenzaldehyde
Bromochloronitrobenzene
6-chloro-3,5-diamino-2-pyrazine-carboxamide
3-Methyl benzyl chloride
Sodium 1-propanesulfonate
BOC-(R)-3-AMINO-4-(4-CYANO-PHENYL)-BUTYRIC ACID
1,1-Cyclohexane Diacetic Anhydride
n-Octylzinc broMide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
3-BroMo-1,1,1-trifluoro-2-propanol, 96%
4-BroMoMethyl-3-nitrobenzoic acid
N-EthylMorpholine
2-AMino-N-cyclohexyl-N-MethylbenzenesulfonaMide
D-tert-Butylglycine
(1S, 2S)-1,2-Bis(4-nitrophenyl)ethylenediaMine dihydrochloride
4-BroMobenziMidaMide, HCl
6-Bromo-benzothiazol-2-ylamine
1H-Benzimidazole-5-carbaldehyde , 97%
2-Bromo-5-nitrobenzoic acid
4-formylbenzamide(SALTDATA: FREE)