HTR8细胞,人绒毛膜滋养层细胞系选用什么培养基 货号:GD-C14520530
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4'-Methoxy-3'-(trifluoroMethyl)acetophenone, 97%
2-BroMophenol
6-BroMo-2-chloro-3-Methoxyphenylboronic acid
2,4-Dichloropyridine-5-boronic acid hydrate
Desogestrel
3-Phenylbenzaldehyde
Molecular sieves, -8+12 beads (Linde 4A)
Quinoline-7-carboxylic acid
3-Methylbenzotrifluoride 98%
Imidazole, sodium derivative
Cesium dodecahydrododecaborate, min. 98%
PERIODIC ACID
Analgin
Butyraldehyde oxime
3-Iodobenzoic acid
N1-[4-Cyano-2-(trifluoroMethoxy)phenyl]acetaMide
Difluoroacetic Anhydride
N-Propyl 2-broMobenzaMide
4'-Isopropylacetophenone, 96%
Tribasic CalciuM Phosphate, Powder, NF
N-CyclohexylforMaMide
Triphenylphosphine oxide
trans-2-hexenal
Vat Violet 1
3-Hydroxy-4-methoxycinnamic acid
N,N'-Diethylthiourea
4-PHOSPHONOOXY-TEMPO HYDRATE
N,N-DiMethylthiourea
4-Chloro-3-fluoropyridine hydrochloride, 97%
2H-Perfluoro-5,8,11,14-tetraMethyl-3,6,9,12,15-pentaoxaoctadecane
Ethyl 4-(n-butylaMino)benzoate, 98%
Astragaloside II
2-AMino-6-nitrobenzoic acid
N-Methyl-2-(2-nitrophenoxy)ethanaMine
3-AMINO-4-(METHYLTHIO)BENZOTRIFLUORIDE
FMoc-D-Ser(tBu)-OH
4-BroMo-N,N-bis(triMethylsilyl)aniline
1-Naphthhydrazide
2-sec-Butyl-3-methoxypyrazine, 99%
2,5-dimethyl-1H-pyrrole-3-carbaldehyde(SALTDATA: FREE)
2-Amino-6-bromoimidazo[1,2-a]pyridine
1-Cbz-4-Aminomethylpiperidine
TERT-OCTYL ISOTHIOCYANATE
(S)-4-ISOPROPYLTHIAZOLIDINE-2-THIONE
N-Formylpiperidine
cis,cis-Muconic acid
N,N-DiMethylsulfaMide
4-BroMothiobenzaMide
tert-Butyl disulfide, 98%
Monobenzone, USP
DecyltriMethylaMMoniuM Chloride
2-butanethiol
Lactulose
a-Methyl-L-p-tryosine
Anisoin
2-Chloroethyl isocyanate
Benzyl DL-Mandelate , 98%
PICENE
Boc-Asn-ONp
4-Hydroxybenzoyl-1-methylpiperzine
2-Fluoro-4-methylbenzonitirile
Bromodifluoroacetyl fluoride
2,5-Diformylfuran
Chloromethyltrimethoxysilane
2-Ethylhexyl mercaptoacetate
Triazol-3-amine
GLYCOCHOLIC ACID SODIUM SALT
DL-2,4-DiaMinobutyric acid dihydrochloride
1-Fluoronaphthalene
Elaidic Anhydride
1-(Oxan-2-yl)pyrazole-5-carbaldehyde
3-Chloro-1-Methyl-1H-pyrazole, 97%
BROMOMETHANE
1,3,5-Trithiane
Cyclohexanecarboxamide
Molybdic acid
Tetrafluorophthalonitrile
cis-4-hydroxy-L-proline
Renin ELISA Kit
Mouse L-Phenylalanine ammonla-lyase,PAL ELISA Kit
Human Helicobacter pylori IgM,Hp-IgM ELISA Kit
Human PLAGL1 ELISA Kit
Monkey ApoA1 (Apolipoprotein A1) ELISA Kit
IFN-γ ELISA Kit
Chicken Hyaluronic acid,HA ELISA Kit
PKCd ELISA Kit
Human PAFAHIB3 (Platelet Activating Factor Acetylhydrolase Ib3) ELISA Kit
ACS ELISA Kit
用于样品制备
Peptone-Salt Solution
SodiuM vinylsulfonate, tech. ca 25% aq. soln.
Bergapten
4-n-Octyloxybenzeneboronic acid, 97%
2-Iodobenzyl Alcohol
Triphenylphosphine oxide
3,7-dimethyl-1-propargylxanthine
silver bifluoride
Decamethylcyclopentasiloxane
Lithium bis(trimethylsilyl)amide
D-Glutamine
(S)-(-)-1-Benzyloxycarbonyl-3-hydroxypyrrolidine, 95%
trans-1,2-Cyclohexanedicarboxylic acid, 98%
2,4,6-TrifluoropyriMidine, 98%
Isoindoline
LacMoid
Glutaric anhydride
CalciuM Methoxide
2-ETHOXY-4-METHYLPHENOL
3,5-Dichlorobenzenesulfonyl chloride
Bis(?dimethylamino)tetrakis (dimethylamino)digallium, 98%
Cyclohexylmethylhydrazine hydrochloride
2-[(tert-Butoxycarbonyl)amino]butanoic acid
Fmoc-Nalpha-methyl-O-t-butyl-L-serine