HTR8-S/Vneo人绒毛膜滋养层细胞系完全培养基 货号:GD-CP119646
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
FMoc-Lys(Z)-OH
25-HydroxyvitaMin D2
CalciuM gluconate
3-Chloro-2-Methylpropionitrile
6,7-Methylenedioxy-4-Methyl-3-MaleiMidocouMarin [for HPLC Labeling], 98%
2,2,3,3-Tetrafluoropropyl Acrylate (stabilized with MEHQ)
3-Acetyl-6-bromo-2H-chromen-2-one , 97%
3-(n-Amyl)-2,4-pentanedione
3-Amino-N-cyclopropylbenzenesulphonamide 97%
D-Arabinose-5-phosphate disodium salt
4-CHLORO-6-METHYL-2-(TRIFLUOROMETHYL)QUINOLINE
5-NITRO-2-FUROYL CHLORIDE
SUCROSE MONOLAURATE
ALLYL N-PROPYL SULFIDE
PotassiuM diphosphate
1-BroMo-3-chloropropane
(3R)-(+)-3-(EthylaMino)pyrrolidine
4'-Chlorobiphenyl-3-carboxylic acid
2-Fluoro-5-Methoxyphenylboronic acid, 95%
CARBOFURAN
Triarachidin
4-Methoxypyridine-N-oxide
Mercury(II) sulfate
1,3,5-trimethylcyclohexane
2,6-Dichloro-4-nitrophenol
1,1'-Sulfonyldiimidazole
1,2,3,5-Tetrafluorobenzene
Triisopropylsilane
PotassiuM tetraborate tetrahydrate, 99%
Indole-4-boronic acid pinacol ester, 97+%
Di-2-pyridyl thionocarbonate, 98%
Tris(4-Methoxyphenyl)phosphine
4-DiMethylaMinobenzoic anhydride
N,N-DiMethyl 4-fluorobenzaMide
3-(Cyclopentyloxy)-4-Methoxyphenylboronic acid
3-(PyrrolidinoMethyl)phenylboronic acid, pinacol ester
AMMoniuM hydrogen sulfate
2-Fluoro-4-(trifluoromethyl)benzamide
4-Bromo-2,2-diphenylbutyric Acid
1-(4-Chloro-3-trifluoromethylphenyl)
Adenosine 5'-monophosphate sodium salt, 99%
4-CHLORO-2-FLUORO-6-IODOANILINE
1-(3,5-DICHLORO-4-PYRIDYL)
Granisetron hydrochloride
Methyl 4-chlorocarbonylbenzoate
Methylboronic acid (contains varying aMounts of Anhydride)
Meso-1,2:3,4-Diepoxybutane
Ethyl 2-(3-broMophenoxy)acetate
Trifluoroacetic acid, silver salt, 98%
PPO, Scintillation Grade
N-(2-PyridylMethyl)glycine Ethyl Ester
2,6-Dinitro-p-cresol
Furo[3,4-b]pyridin-5(7H)-one
4-Ethylacetophenone
Decanoic acid
3-Methyl-2-butanone
Guanylurea phosphate
1-ETHYNYLPYRENE
1-CYCLOHEXYL-3-(2-MORPHOLINOETHYL)CARBODIIMIDE METHO-P-TOLUENESULFONATE
N-Methyl-N-(3-pyridin-4-yl)benzylamine 97%
2,4-Dimethoxyphenyl isocyanate, 97%
Octafluoro-4,4'-biphenol
trans-Diethyl caronate, 95%
N-ACETYL-2-FLUORO-DL-PHENYLALANINE
3-CHLORO-2,4,5,6-TETRAFLUOROBENZOIC ACID
CYCLOHEXYLDIPHENYLPHOSPHINE OXIDE
1,2-EPOXYHEXADECANE
DL-Phenylsuccinic acid
2,2'-Bis(2-oxazoline)
Ethyl 5-(2,4-dichlorophenyl)oxazole-4-carboxylate
Acetophenone-d8, for NMR, 99+ atoM% D
Methanesulfinic acid, sodiuM salt, 85%
ChloroiodoMethane (stabilized with Copper chip)
Voglibose
BETAINE MONOHYDRATE
4-Bromo-2-(trifluoromethoxy)benzaldehyde
1-(3-Chloro-2-pyridyl)
benzyl (S)-(+)-mandelate
FⅡ ELISA Kit
BAFFR ELISA Kit
Human Amylin ELISA Kit
Human RETN ELISA Kit
MHC II/RLA II ELISA Kit
IL-6R ELISA Kit
IFNβ ELISA Kit
Human toxic shock syndrome toxin,TSST-1 ELISA Kit
Human AT ELISA Kit
Mouse C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit
Peptococcus Selective Agar
Azospirillum Medium
Ethyl 4-chloro-7-Methoxyquinoline-3-carboxylate, 97%
trans-4-n-Butylcyclohexanecarboxylic acid, 99%
4-AcetaMidobenzenesulfonyl chloride, 98+%
2-AMino-Oxazole
4-Methoxypyridine-N-oxide
4-Chloro-3-nitrobenzaldehyde
2-Chloro-3-cyano-4,6-dimethylpyridine
3-Chlorobenzyl chloride
Zinc naphthenate
AMMoniuM Citrate Tribasic
(4-AMinophenylthio)acetic acid, 97%
N-[3-(TrifluoroMethyl)phenylsulfonyl]-DL-leucine, 96%
3-Indazolinone, 97%
Methylhydroquinone
2,2'-Bis(2-chlorophenyl)-4,4',5,5'-tetraphenyl-1,2'-biiMidazole
3,4,5-TriMethoxyphenylboronic acid
BroMopentafluorobenzene
4-Fluorophenylacetylene
2-Cyano-3-Methylpyridine-5-boronic acid, pinacol ester
N-Acetyl-L-valine
Phenylsulphur pentafluoride
alpha-Methyllevulinic Acid
2-sec-Butylcyclohexanone (mixture of isomers)