HTR-8/Svneo人绒毛膜滋养层细胞系液体培养基 货号：GD-CP138897
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 3-(4-fluorophenyl)-5-Methylisoxazole-4-carboxylate, 97%
3,5-Dinitrobenzotrifluoride
1,3-BIS(METHYLTHIO)-2-PROPANOL
Sarcosine Methyl ester hydrochloride
3-ACETAMIDOPYRIDINE
3-CHLOROBENZO[B]THIOPHENE-2-CARBOXYLIC ACID
4-BroMo-1-(phenylsulfonyl)pyrazole
2-Phenylpiperidine hydrochloride
3,5-Dichloro-2-pyridinol, 98%
1H-Indazol-4-ol ,97%
5-Methyl-2-phenyl-2H-pyrazol-3-ol
Bis(cyclohexanone)oxaldihydrazone
2-IODOPHENYLACETONITRILE
4-Aminoveratrole
phellodendrine
Benzyloxyacetaldehyde
2,8-Dichlorocyclooctanone
N-Cyclopropyl 2-broMo-4-trifluoroMethoxybenzenesulfonaMide
ThioMorpholine, 97%
O-TOLIDINE
5-Carboxyfluorescein Hydrate
embelin from embelia seeds
7-methoxy-2-naphthol
1-butylpyrrolidine
toluene-2-sulphonyl chloride
Benzyl glycinate hydrochloride
D-NORVALINOL
4-Fluoro-2-Hydroxyacetophenone
3,5-Di-tert-butylsalicylic acid, 97%
4-(4-MorpholinylMethyl)benzoic acid, 97%
(+)－Catechin Hydrate
Phthaloyl chloride
4-(TrifluoroMethoxy)benzenesulfonaMide
Sandalwood oil
CYCLOPENTYLACETIC ACID
3-Methoxy-2-naphthol
3-Bromo-4-chlorobenzoic acid
9-Decen-1-ol
1-(2-Bromo-ethoxy)-3,5-dimethyl-benzene
4-Phenoxypyridine
1-Butyl-3-methylimidazolium tetrafluoroborate
Trimethyl-1-propanaminium iodide
5-ETHOXY-2-MERCAPTOBENZIMIDAZOLE
2-Aminoethyl(ethyl)amine
2-EthyliMidazole
tetronic acid
2,3,4,6-Tetra-O-benzyl-alpha-D-glucopyranosyl p-TrifluoroMethylbenzylthio-N-(p-trifluoroMethylphenyl)forMiMidate
2-Chlorophenylhydrazine hydrochloride, 97%
4-Fluorobenzophenone, 98%
Bis(trimethylsilyl)trifluoroacetamide
Decanoic Anhydride
(S)-2-Amino-3-Cyclohexyl-Propan-1-ol
(2Z)-3-(3,4-dimethoxyphenyl)-2-sulfanylprop-2-enoic acid
dichloroisocyanuric acid sodium salt dihydrate
Dimethylbenzene Sulfonic Acid
(ETHYLTHIO)ACETIC ACID
3,5-DICHLOROBENZOIC ACID METHYL ESTER
2,6-Dichloronicotinic acid
Tetrahydrofurfuryl alcohol
Chloroplatinic acid hexahydrate ,99.9% [trace metal basis, 38 to 40% Pt]
Magnesium hexafluoroacetylacetonate dihydrate, min. 97%
Protoporphyrin IX, dimethyl ester
1,3-Benzothiazole-6-carboxaldehyde, 97%
4-Fluoromandelic acid
Fasudil hydrochloride
(S)-(-)-alpha-Methoxy-alpha-(trifluoromethyl)phenylacetic acid
Acetamidine hydrochloride
2,6-Dichloro-trans-cinnamic acid
1,3-Bis(2,6-diisopropylphenyl)iMidazol-2-ylidene
3,5-DiMethyl-1-(THP)pyrazole-4-boronic acid
D-Alanine Methyl ester hydrochloride, 98%
1-Iodonaphthalene, 97.5%
3,3-DiphenylpropylaMine
3,3-Diphenylpropionic Acid
CYCLOHEXANE-1,1-DICARBOXYLIC ACID
N,N-diphenyl-1H-indol-4-amine
6-BENZYL-5,7-DIOXO-OCTAHYDROPYRROLO[3,4-B] PYRIDINE
Astragaloside IV
VIP ELISA Kit
GABA ELISA Kit
Human lipolysaccharide binding protein,LBP ELISA Kit
Human SPARC (Secreted Protein Acidic And Rich In Cysteine) ELISA Kit
HSP-72 ELISA Kit
Rat NTXI (Cross Linked N-Telopeptide of Type I Collagen) ELISA Kit
MIF ELISA Kit
Human Perforin/Pore-forming protein，PF/PFP ELISA Kit
Human Androstenedione,ASD ELISA Kit
OMT ELISA Kit
Wallerstein Laboratory Nutrient Agar
Glucose Asparagine Agar(Waksman, 1961)
Indole-3-Butyric Acid
Lead chromate
5-AMino-4-broMo-3-Methyl-1H-pyrazole hydrobroMide, 98%
4-HYDROXYBUTYL METHACRYLATE
embelin from embelia seeds
Octyltrimethylammonium chloride
Sodium carbonate
Rutin
4-Butylbenzoic acid
Triethylamine hydrochloride
LithiuM Triflate
Ethyl 3-trifluoroMethyl-1H-pyrazole-4-carboxylate, 97%
CeriuM(III) carbonate hydrate, REacton|r, 99.999% (REO)
LithiuM tri-tert-butoxyaluMinuM hydride
Boc-3-(1-naphthyl)-D-Alanine
1-BOC-4-(4-broMo-3,5-diMethylphenoxy)piperidine
4-Methyl-1-pentanol
RhodiuM (III) chloride trihydrate
3-(2-Methyl-1,3-dioxolan-2-yl)phenylboronic acid
5-Chloromethyl-3-(4-methoxyphenyl)-[1,2,4]oxadiazole
N2-Benzoyl-L-arginine ethyl ester hydrochloride
2-Methylthio-2-imidazoline hydriodide, 99%
3-Chloro-1H-indazol-5-amine ,97%
