CTSC-3细胞,人舌癌细胞系选用什么培养基 货号:GD-C14518920
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞,为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1-BroMo-3-nitro-4-(trifluoroMethoxy)benzene, 95%
(±)-2-Methyl-1-butanol
3-PHENOXYPHENYLACETONITRILE
4-TERT-OCTYLRESORCINOL
7-Chloro-4-iodoquinoline
(1R,2R)-TRANS-1,2-CYCLOHEXANEDIOL
4-Azatricyclo[4.3.1.1~3,8~]undecan-5-one hydrochloride
3-(1,3-dihydro-2H-isoindol-2-yl)benzoic acid(SALTDATA: FREE)
Methyl imidazo[1,2-a]pyrazine-8-carboxylate
1-(2-Bromo-ethyl)-3-methyl-benzene
2-Chloro-6-fluoro-4-(trifluoromethyl)phenol 98%
2-[(4,6-DIMETHOXYPYRIMIDIN-2-YL)THIO]BENZOIC ACID
METHYL 2-FLUOROBENZOATE
3-(2,4-DICHLOROPHENYL)PROPIONIC ACID
Methyl 1-aminocyclopropanecarboxylate hydrochloride
TetrabutylaMMoniuM hydrogensulfate
Di-n-octyl Sulfoxide
2,4-DibroMo-5-fluoroanisole
Tin(IV) acetate, 98%, extra pure
Papaverine Hydrochloride, USP
Iron(II) Phthalocyanine
2,5-Dichlorophenol
Silane coupler KH-791
5-Amino-4-bromobenzothiazole
DL-Pyroglutamic acid
HEXAHYDRATE
1-(3-BroMophenyl)
2-BroMo-4-fluoro-N-(3-fluorobenzyl)aniline, 97%
2-Chloro-3,5-dinitrobenzoic acid, 97% (dry wt.), May cont. up to ca 5% water
3-(4-BroMophenyl)-1H-pyrazole, 97%
3-BroMo-2-nitropyridine
TRANS,CIS-2,6-NONADIEN-1-OL
2,3,5,6-Tetrafluoro-4-hydroxybenzoic acid hydrate
RuCl2[(S)-(DM-BINAP)][(S,S)-DPEN]
6-BroMo-7-Methyl-3-nitroiMidazo[1,2-a]pyridine
DL-Asparagine Monohydrate
Hexaphenylcyclotrisiloxane
2,3:4,5-Di-O-isopropylidene-beta-D-fructopyranose
(4'-Methoxy-biphenyl-3-yl)-acetic acid
2-(Aminomethyl)-5-fluorobenzeneboronic acid hydrochloride
2-(2-METHYLPHENYL)PYRROLIDINE
5-Methylnicotinic acid
2,6-Dinitro-p-cresol
4-Amino-m-cresol
2-Phenyl-2-iMidazoline
4-(2-BroMoethyl)benzenesulfonic Acid
4-chloro-2-fluorophenylboronic aicd, pinacol ester
beta-Tetralone, 95%
Ethyl dichlorophosphite, 98%
3-AMino-1-tert-butoxycarbonylpiperidine
3-AMino-1-propanol Hydrochloride
1-Adamantanamine hydrochloride
Cyclopenta[c]pyrrole-1-carboxylic acid, (2S)-2-cyclohexyl-N-(pyrazinylcarbonyl)glycyl-3-methyl-L-valyloctahydro-, ethyl ester, (1S,3aR,6aS)-
(2S)-2-(tert-butylcarbamoylamino)-3,3-dimethyl-butanoic acid
2,2-Bis[4-(4-aminophenoxy)phenyl]-hexafluoropropanane
DIPROPYL PHTHALATE
2-PhthaliMidopropionic acid
1-N-HEPTYLOXY-4-NITROBENZENE
2-Methyl-3-nitroaniline
6-Methoxy-1H-pyrrolo[2,3-b]pyridine
2-(2-Chloroethoxy)tetrahydro-2H-pyran
1-(tert-Butoxycarbonyl)pyrrolidine-2-carboxylic acid
5-methyl-1,3-diphenyl-1H-pyrazole-4-carboxylic acid(SALTDATA: FREE)
n-Octyl Sulfide
Metazachlor
Butyl lactate
Allyl mercaptan
2-Methoxy-5-fluoropyridine
1,3-Bis(trifluoroMethyl)-5-broMobenzene
N-[3-[3-(DiMethylaMino)acryloyl]phenyl]-N-ethylacetaMide
4-(2-Chlorophenyl)benzaldehyde
1,2-DibroMocyclopentene, 97%
Xylazine Hydrochloride, USP
3,5-DibroMopyridine
Methallyl acetate
Ethyl 4-formyl-1H-pyrrole-2-carboxylate
Calcium folinatc
Decamethylcyclopentasiloxane
SEMA4D/CD100 ELISA Kit
Mouse β-glucuronidase,βGD ELISA Kit
Lp-PLA2 ELISA Kit
CoQ10 ELISA Kit
ANXA5 ELISA Kit
Rat L-Phenylalanine ammonla-lyase,PAL ELISA Kit
TRAIL-R3 ELISA Kit
Human PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
Human Trichinella antibody ELISA Kit
PGC ELISA Kit
Schwartz Differential Medium
Pseudomonas Agar for Determine of Pyocyanin
YtterbiuM sputtering target, 76.2MM dia x 1.59MM thick
ChroMotrope FB
5-AMino-2-cyano-3-Methoxypyridine, 96%
2-Methyl-1,3-dithiane, 98%
2,5-Dichlorophenol
Methyl 3-hydroxyisonicotinate
Methyl-D3-p-Toluenesulfonate
Sulfadimethoxypyrimidine
Prenyl acetate
(Trifluoromethyl)trimethylsilane
Triphenyltin Acetate
2,3-Dihydroxypyridine, 98%
CyclopropyldiphenylMethanol, 98+%
4-Chloro-4'-hydroxybenzophenone
BroMoethane
2-(CYCLOHEXYLAMINO)PYRIDINE-5-BORONIC ACID PINACOL ESTER
0
Methyl 9-fluorenylideneacetate
Benzene, 1,3,5-trifluoro-2-Methoxy- (9CI)
1,3-Bis(3-aminopropyl)tetramethyldisiloxane ,97%
3-Methyl-5-phenyl-4-isoxazolecarboxylic acid, 97%
2,4-Diacetyl deuteroporphyrin IX dimethyl ester
4-(Trifluoromethyl)benzophenone 97%