COS-1细胞系——非洲绿猴SV40转化的肾细胞系消化注意事项 货号:GD-C11519302
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 3-ethoxy-2-butenoate, 97%
β-Alanine Methyl ester hydrochloride
3-Fluoro-4-nitrophenol
Boc-Arg(Tos)-OH
D(+)-Trehalose dihydrate
3-AMino-4-Methylphenylboronic acid, HCl
4-Nitrophenol
2-Bromobenzylmercaptan
Ethyl 4,6-dichloro-3-formyl-1H-indole-2-carboxylate , 97%
2-(4-Bromophenyl)-1,1-diphenylethylene
N-Acetyl-L-tyrosine Ethyl Ester Monohydrate
3-CYANOMETHYLPHENYLBORONIC ACID
2-BROMO-1,1,2-TRIFLUOROETHYL ETHYL ETHER
4-(METHYLSULFONYL)BENZONITRILE
3-Chloropyrazine-2-carbonitrile
1H-Benzotriazole
3,5-DibroMo-2-Methylthiophene
Methyl 2-(4-broMo-2-nitrophenyl)acetate
6-(BroMoMethyl)-3,4-dihydro-2H-1,5-benzodioxepine, 97%
Testosterone Benzoate (CIII)
3-Nitrophenylacetic Acid
4-Nitrothioanisole
2-amino-7-bromofluorene
N-Methylpropionamide
1,4-Dioxan-2,5-dione
Niobium oxide
1-Fluoro-3-iodobenzene
2-AMino-4-(TrifluoroMethyl)PyriMidine
(S)-(+)-3-AMino-1-(benzyloxycarbonyl)pyrrolidine, 96%
7-Methoxy-4-Methyl-2(1H)-quinolinone, 96%
5-AMino-2,4-difluorobenzoic acid, 98%
1,3-Dichlorobenzene
3,3′-Dipropylthiacarbocyanine iodide
N-p-Tosylglycine
4-MethoxybenzaMide
SulfabenzaMide
2-Hydroxyethyl methacrylate, stabilized, 97%
Sodium nitrite, 97+% (ACS)
5-Chloropyrazine-2-carbonitrile
Methyl 5-(Hydroxymethyl)-1H-imidazole-4-carboxylate
Tetrasodium Ethylenediaminetetraacetate Dihydrate
Ethyl formate
N,N-DIPHENYLFORMAMIDE
3-(TRIFLUOROMETHYL)BENZOPHENONE
2-Acetoacetoxyethyl Methacrylate
1-BroMo-2-ethylbutane
4,4-DiMethylcyclohexanone
N-Phenyl 5-broMo-2-chlorobenzaMide
2,3-Dihydro-1,4-benzodioxine-6-carbaldehyde, 97%
Tretinoin, Powder, USP
HistaMine Diphosphate Hydrate
2-Acetyl-1-naphthol
4-(4-Fluorophenyl)piperidine hydrochloride
Dodecylbenzenesulfonic Acid
trielaidin (C18;1),(trans)-9
Diethyl pyrocarbonate
TRIFLUOROACETYL TRIFLATE
3-BroMo-5-isobutoxyphenylboronic acid, pinacol ester
1-CHLORO-3,3-DIMETHYLBUTANE
4-Bromo-o-xylene
Titanium(IV) fluoride, 98%
Erbium(III) iodide, anhydrous (99.9%-Er) (REO)
Sodium 2-Thiobarbiturate
Dihydromyrcene
4,4'-Bis(methoxycarbonly)-2,2'-bipyridine
ROSARIN
2,5-DICHLOROTHIOPHENOL
2-Nitrotoluene
1-Butylhydantoin
2-Butoxy-5-(trifluoroMethyl)phenylboronic acid
alpha-Chloralose, 97%
Methyl 3-aMinobenzo[b]thiophene-2-carboxylate, 97%
4-Methoxy-2-(triMethylsilyl)phenyl TrifluoroMethanesulfonate
2-Methylindole-3-carboxaMide
2-iodonaphthalene
[2-(4-benzylpiperazin-1-yl)phenyl]methanol
Ractopamine hydrochloride
2-fluoro-5-methylbenzonitrile
RLR-9 ELISA Kit
Pentodine ELISA Kit
Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
Human dihydropyrimidinase-like 2,DPYSL2 ELISA Kit
PROG ELISA Kit
β-EP ELISA Kit
ET ELISA Kit
Human PROS ELISA Kit
Human Thromboxane A2,TX-A2 ELISA Kit
progestogen ELISA Kit
Indole Nitrite Medium
Lead Acetate Medium
HafniuM(IV) 2,4-pentanedionate, 97%
DysprosiuM(III) fluoride, anhydrous, 99.9% (REO)
5-(5-BroMo-3-pyridyl)-1H-tetrazole, 95%
Hexyl Trans-2-Butenoate
4-Nitrothioanisole
2,3,4,5,6-PENTABROMOANILINE
thyrocalcitonin rat
m-Tolylhydrazine
3-Aminoisonicotinic acid
N-[5-OXO-1-(2,4,6-TRICHLOROPHENYL)-2-PYRAZOLIN-3-YL]-3-[2-(2,4-DI-T-PENTYLPHENOXY)HEXANAMIDO]BENZAMIDE
5-Cyanoindole
5-AMino-3-(4-Methoxyphenyl)-1H-pyrazole, 97%
1-Chloro-3-fluorobenzene, 99%
3′,4′-(Methylenedioxy)acetophenone
Crotonaldehyde, Mixture of cis and trans
5-Cyano-2-pyridinecarboxylic acid
4-(TrifluoroMethyl)benzylaMine
Cyclopropyl broMide
(4-AcetoxyMethyl)phenylboronic acid
4-bromo-2-chloro-1-isocyanatobenzene(SALTDATA: FREE)
3-Dimethylamino-1-propanol, 99%
2-(4-Biphenylyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane
N-(4-Methoxybenzyl)pyridin-2-amine