COS-7细胞系——非洲绿猴SV40转化的肾细胞系消化注意事项 货号:GD-C11518570
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞,而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞,加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-BroMothiophene-2-carboxaldehyde, 94%
3,5-Heptanedione
N-TERT-BUTYL-3,5-DIMETHYLANILINE
2,4-DICHLORO-1-NAPHTHOL
N-DODECYLPHOSPHONIC ACID
TROPONE
XtalFluor-E
2',4'-Difluoropropiophenone
4-Diazo-N-ethyl-N-(2-hydroxyethyl)aniline Chloride Zinc Chloride
2-Fluorobenzotrichloride 99%
5-Acetamido-2-nitrobenzeneboronic acid 96%
Panthenol
4,6-Dimethyl-2-pyrone
D-Histidine hydrochloride monohydrate
4-Oxo-4H-1-benzopyran-2-carboxylic acid
5-BroMo-1,2-diMethyl-1H-iMidazole
2,5-DiMethoxy-1,4-benzoquinone
3-(3-BroMophenyl)-5-(dichloroMethyl)-1,2,4-oxadiazole
Silver, 99.9%, foil, 25x25 MM, 1.0 MM thick, 1ea = 6.6gr
GAMMA-BHC
Tris(4-nitrophenyl)aMine
3-Fluorobenzotrifluoride
(R)-(-)-alpha-Methoxyphenylacetic acid
2-Chlorobenzothiazole
Bis(2-ethylhexyl) adipate
3,4-Dichlorophenylboronic acid
MEZEREIN
2-BroMo-5-Nitrobenzoic Acid
Methyl 4-iodo-2-Methoxybenzoate, 98+%
2,3,4,5-Tetrafluorobenzyl alcohol, 97+%
Aniline Blue solution
SodiuM polyacrylate
Isooctyl acrylate
Tigecycline
TRANS-3-ETHOXYCINNAMIC ACID
(-)-MENTHYL CHLOROFORMATE
4-(Hexafluoro-2-hydroxyisopropyl)aniline
3-Methylthiophene-2-carboxaldehyde oxime
2,3-Dichlorophenylboronic acid
4-(1,3-Thiazolan-2-yl)pyridine
PERFLUOROPENTANOIC ACID
(R)-2-Amino-2-methyl-4-pentenoic acid
3,3-Diphenyl-D-alanine
Piperidine;Hexahydropyridine;PentaMethyleneaMine
2-AMino-4-MethylbenzaMide
CarbendaziM
Mono-6-O-Mesitylenesulfonyl-gaMMa-cyclodextrin
3,4-Difluoro-2-hydroxyaniline
4,5-DibroMo-2-phenyl-2,3-dihydropyridazin-3-one, 97%
Acid Yellow 23
2-(2-Benzothiazolyl)-3,5-diphenyltetrazoliuM BroMide
Barium hydride
3,5-Difluoro-L-phenylalanine
DL-alpha-Tocopherol
2-(2-Bromoethoxy)anisole
Boc-3-(2-Naphthyl)-D-alanine
6-(Piperidin-1-yl)pyridine-3-boronic acid
2-(TrifluoroMethyl)benzonitrile
4,4'-DIBROMO-TRANS-STILBENE
6-AcetaMidohexanoic acid
4-Hydroxy-2,3,5,6-tetrafluorobenzotrifluoride
2-Chlorophenyl isocyanate
N-(4-Oxocyclohexyl)phthalimide
3-[4-(tert-Butyl)phenyl]-5-(chloromethyl)-1,2,4-oxadiazole, Tech.
SODIUM TITANATE
Pentadecanedioic acid
2,4-Dihydroxypyridine
1-Octadecanol
(S)-(-)-alpha-Methoxy-alpha-(trifluoroMethyl)phenylacetic acid
3,4'-DiaMino-4-nitrodiphenyl Ether
Vinylferrocene
Tris(triphenylphosphine)rhodiuM(I) chloride, 99.99%, (trace Metal basis)
SodiuM Alendronate, EP
3,5-Difluoroanisole
Alpha,Alpha'-Dibromo-p-xylene
5,6-dihydropyridin-2(1H)-one
Adipic dihydrazide
2-Fluorobenzonitrile
F-TESTO ELISA Kit
C.albicans ELISA Kit
Human Netrin-1,Ntn1 ELISA Kit
Human IRF8 ELISA Kit
GSR ELISA Kit
PⅢNP ELISA Kit
MHC I/H-2 I ELISA Kit
Human C3a ELISA Kit
AC ELISA Kit
PTGES ELISA Kit
Skirrow Agar Base
CATC Agar
1-Butanol, For Analysis
Cyclosporin A
5-Methoxy-1-indanone, 98%
2-Methoxycrotonic acid, 98%
3-Fluorobenzotrifluoride
[1,2,4]Triazolo[1,5-a]pyridine,6-bromo-2-methyl-
Methyl pyrazine-2-carboxylate
4-(4-methoxyphenyl)butan-2-one
N-Methylaniline
PotassiuM DichroMate
2-Dicyclohexylphosphino-2',4',6'-triisopropyl-3,6-diMethoxybiphenyl, 97+%
2,3-Difluoro-4-Methylbenzyl broMide, 97%
Iodine, 99+%
N,N-DiMethyl-p-phenylenediaMine sulfate salt
4-(2-Ethoxycarbonylethyl)phenylboronic acid
2-AMinobiphenyl-3-carboxylic acid
2-(Tributylstannyl)pyrazine
AMMoniuM hexafluoroaluMinate
1,3-DITHIOLANE
6-Chloroimidazo[2,1-b][1,3]thiazole-5-carboxaldehyde
trans-Styrylacetic acid, 96%
(R)-1-(2-Bromophenyl)ethylamine
(-)-cis-Myrtanylamine, 98%