Calu-1细胞|人肺癌细胞系什么细胞 货号:GD-C10519097
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethylene glycol diMethacrylate, 98%, stab. with 100ppM 4-Methoxyphenol
Chloroacetic Acid tert-Butyl Ester
2,3,4,5,6-PENTAMETHYLBENZYL ALCOHOL
Alverine Citrate
5-CHLOROBENZOFURAN-2-CARBOXYLIC ACID
1-(4-BroMophenyl)piperidin-4-one
4-(3-ChloropropylcarbaMoyl)phenylboronic acid
Cyclohexanecarboxylic acid ,99%
1-Iodo-2-methylpropane, stabilized, 97%
1-Acetoxy-1,1,3,3,5,5,-hexamethyltrisiloxane
Aluminium sulfate, extra pure, (trace metal basis), 99.999%
4-(PYRIMIDIN-5-YL)BENZALDEHYDE
BOC-(R)-3-AMINO-4-(2-CHLORO-PHENYL)-BUTYRIC ACID
4-HEPTANOL
(S)-(-)-1-PhenylethylaMine
3-Chlorobenzoic acid
DiMethyl Hexadecanedioate
N-(3-Methoxypropyl) 4-broMo-3-trifluoroMethylbenzenesulfonaMide
Tri-n-octylaMine, 97%
Quinine Sulfate, Dihydrate, USP
1-(Triisopropylsilyl)pyrrole
3-Ethoxybenzoic acid
(R)-3-Cyclohexene-1-carboxylic acid
P-amino-L-phenylalanine
3-Fluoro-2-nitrophenol
LEAD TITANIUM OXIDE
3-Nitrobenzonitrile
7-Fluoro-6-nitro-4-hydroxyquinazoline
2-Isopropylnaphthalene, 96%
Silicone oil, for oil baths, usable range froM -40 to +200°C
3,3-DiMethoxy-2-butanone
GalliuM nitrate hydrate
2-BROMO-5-IODOBENZOIC ACID
PINONIC ACID
4,4'-Oxydiphenol
3-ForMyl-4-isopropoxyphenylboronic acid
2,2',4,4'-Tetrahydroxybenzophenone
Decafluorobiphenyl
Hemimellitic Acid Hydrate
3-Undecyne
4-Acetamidophenol
3'-Fluoroacetophenone
Bis(pentamethylcyclopentadienyl)zirconium dichloride
FILIPIN III
1,4-Butanediol
4-(TriMethylsilyl)Morpholine
L-Threonine Benzyl Ester Hydrochloride
2-Methylthiophene, 98%
N-Myristoyl-D-erythro-sphingosine, 99+%, synthetical
Tricine
4-MethoxypyriMidine
3,4-Diaminopyridine
4-(Tert-Butyldimethylsilyloxy)Phenol
curdlan from alcaligenes faecalis
Z-dl-asparagine
Barium molybdate
Methylhexahydrophthalic anhydride
2,3,4-Trifluorobenzoic acid
Methyl 4-aMino-3-iodobenzoate
2-Chloro-4-fluoronitrobenzene
(2S)-2-{[(tert-Butoxycarbonyl)amino]methyl}-3-methylbutanoic acid
N-BUTYL NITRATE
2-Hydroxyisonicotinic acid
N-Fmoc-S-(4-methoxybenzyl)-L-cysteine
Ethyl nicotinoate
2,3-DichlorophenethylaMine
18-Crown-5 [4-(2,4-Dinitrophenylazo)phenol]
3-(TetrahydrofurfurylaMinocarbonyl)phenylboronic acid pinacol ester
IsoaMyl butyrate, 99+%
4-Hydroxy-2-Mercapto-6-MethylpyriMidine, 98%
Methyl 3,4-Dihydroxybenzoate
N,N,N',N'-tetraethylsulfamide
Isovaleryl chloride
N-[(1,1-dimethylethoxy)carbonyl]-L-leucine
Biotin pentafluorophenyl ester
U-II ELISA Kit
α2M ELISA Kit
Human Presenilin 2,PS-2 ELISA Kit
Human CMA1 ELISA Kit
E2 ELISA Kit
Thy1 ELISA Kit
Chicken Complement 3 split product,C3SP ELISA Kit
Human PRH ELISA Kit
Human VEGFR-3/Flt-4 ELISA Kit
CDH5 ELISA Kit
Murashige & Skoog Vitamins
Bacteroides Bile Esculin Agar Base
AluMinuM nickel isopropoxide, 99.9% (M.b.), 10% w/v in isopropanol, packaged under Ar in resealable CheMSeal^t bottles
Glycine tert-butyl ester, 97%
5-Chlorofuran-2-Carboxylic Acid
2-Fluorobenzyl broMide, 98%
3-Ethoxybenzoic acid
Reactive Brilliant Red X-8b
Soluble Starch
3-fluorosalicylaldehyde
4-(ETHYLTHIO)BENZENEBORONIC ACID
TPSI
Bis(2-ethylhexyl) phosphate, 95%
(S)-(-)-2-(Boc-aMino)-3-benzyloxy-1-propanol, 97%
DI-N-HEXYL SULFIDE
Benzene-1,3,5-triacetic acid
2-Methoxy-5-(trifluoroMethyl)benzonitrile
6-AMinopyridine-3-carboxaMide
1-Butylpiperidin-4-one
3,4-Difluorobenzaldehyde
3-Chloro-5-methylpyridazine 97%
4-Chloroanilidophosphoryl Dichloride
2-Methoxyethyl Chloroformate