GLC-15细胞|人肺癌腺细胞系(买细胞,送话费,多买多送) 货号:GD-C10419248
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(S)-(+)-4-Phenyl-2-oxazolidinone, 98%
HexadecylpyridiniuM chloride Monohydrate
3-(DiMethylaMino)-2-iodoacrolein
2,4-BIS[5,6-DI(P-SULFOPHENYL)-1,2,4-TRIAZIN-3-YL]PYRIDINE TETRASODIUM SALT
2-(tert-ButyldiMethylsilyl)-1,3-dithiane
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)SAMARIUM(III)
TERT-BUTYLPHOSPHONIC ACID
N-BOC-3-Piperidinecarboxylic acid, 98%
2-(4-Chlorophenoxy)isobutyric Acid
Iron disulfide (Iron pyrite), 95%
Bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt, min. 97%
Orphenadrine citrate
Imipramine hydrochloride
Propylthiouracil
2-Methylbenzothiazole
Erythrosin B
2,3-Difluorophenetole
Methyl 4-broMo-2-hydroxybenzoate
Zinc phthalocyanine, 96%
StrontiuM Chloride, Hexahydrate, Crystal, Reagent, ACS
DL-Ornithine Monohydrochloride
2-Isopropylaniline
2,3-Dichlorobenzaldehyde
3-Bromo-N-phenylcarbazole
4-Fluorophenylacetonitrile
1-(TRIFLUOROMETHYL)VINYL ACETATE
1,4-DIBROMOBENZENE-D4
N,N'-(1,4-Phenylene)bis(acetoacetaMide)
TerbiuM(III) fluoride, anhydrous, REacton|r, 99.9% (REO)
Ethyl 2-aMino-5-phenylthiophene-3-carboxylate, 97%
1-(3-Methylbenzyl)
Phorate
Dilauryl thiodipropionate
Octocrilene
N,N,N',N'-TETRAETHYL-1,3-PROPANEDIAMINE
PHENANTHRENE-9-CARBOXALDEHYDE
Dichlorobis(tricyclohexylphosphine)palladium(II) ,98%
2,2,2-Trifluoroethanesulphonyl chloride 97%
1,2-Dichloro-1,1-difluoroethane (HCFC-132b) 97%
tert-Butyldimethylsilane
2-IODO-2-METHYLPROPANE
3-CHLORO-4-FLUOROPHENYLACETONITRILE
6-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE CYCLOHEXYLAMMONIUM SALT
(S)-(+)-2-Amino-3-methyl-1-butanol
Methyl 3-aMino-4-Methylthiophene-2-carboxylate
AMinoMethanesulfonic acid
Heptaethylene Glycol
1-BOC-4-(4-nitrophenyl)
2-Chloro-6-Methylpyrazine, 97%
Potassium bisaccharate
Pyridazine-4-carboxylic Acid
Chloromethyl isopropyl ether
2'-Deoxy-N2-Dimethylaminomethylene-Guanosine
Deoxyadenosine
Dimethylaminohexanol
2,4,5-TRICHLOROBENZENESULFONYL CHLORIDE
(?)-2,3-Bis[(2R,5R)-2,5-diMethylphospholano]-N-MethylMaleiMide(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate
N-BENZOYLIMIDAZOLE
(S)-N-FMoc-(3-Pyridyl)alanine
Vanillic Acid
1-(2,4-Difluorophenyl)biguanide hydrochloride 97%
(S,S)-[2-(4'-i-Propyloxazolin-2'-yl)ferrocenyldiphenylphosphine, min. 97%
Pentolinium tartrate, 98%
2-Nonanone
Methyl 2-chloro-4-fluorobenzoate
7-Chloroindole
3-(4-Hydroxyphenyl)propionic acid
3-BroMo-4-Methylbenzonitrile
1-BroMo-4-cyclohexylbenzene
4-Fluoro-3-hydroxybenzonitrile
Chelerythrine chloride, 98%
4-Methoxyphenylacetyl chloride, 98%
4-BroMobenzotrifluoride
6-AMino-1-hexanol
4-hexen-1-ol
Vorapaxar
Fmoc-D-Dab(Boc)-OH
3,5-Dimethyli-4-isoxazolecarboxamide
RXFP1 ELISA Kit
S100β ELISA Kit
Human PCDHβ16 ELISA Kit
Human farnesyl-diphosphate farnesyltransferase 1,FDFT1 ELISA Kit
OGCHI ELISA Kit
BMG/β2-MG ELISA Kit
Chicken anti-Endomysial Antibody IgA,EMA IgA ELISA Kit
CCK-8 ELISA Kit
Human hemagglutinin,HA ELISA Kit
ACH ELISA Kit
Skimmed Milk Sucrose Peptone Medium Base
Alkaline Peptone Water
Diethyl acetylMethylphosphonate, 97%
Hydrogen Fluoride Pyridine CoMplex
5,5'-DibroMo-BAPTA, 95%
2-Fluoro-4-(trifluoroMethyl)pyridine, 97%
2-Isopropylaniline
Quinalphos
Digitonin
Epoxypropionic acid
DIETHYLTIN DICHLORIDE
Nalidixic acid
1-HexadecylpyridiniuM Chloride Monohydrate
2,6-Dichloro-3-nitropyridine, 97%
3,4-DiMethylbenzeneboronic acid, 98+%
1-BroMo-3,4-(Methylenedioxy)benzene
N-Acetyl-L-glutaMic acid
4-(N,N-DiMethylaMinosulfonyl)phenylboronic acid pinacol ester
2-(4-BroMophenyl)-N-MethylacetaMide
2,5-DiMethyl-3(2H)-furanone
2,2'-DIAMINODIPHENYL SULFIDE
3-Hydroxy-4-methoxybenzaldehyde ,99%
2,6-Diacetylpyridine, 99%
Span 85 (=Sorbitan Trioleate)
2-Aminocyclopent-1-ene-1-carbonitrile