WERI-Rb-1细胞株：人视网膜神经胶质瘤细胞系体外培养

WERI-Rb-1细胞株：人视网膜神经胶质瘤细胞系体外培养 货号：GD-C8219092

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1-[3,5-Bis(trifluoroMethyl)phenyl]-3-(4-fluorophenyl)urea, 97%
N,N-Bis(2-hydroxyethyl)glycine
N-Boc-2-aMinoMethylpyridine
N,N-DiMethylMethacrylaMide , 95%
2-ChloropyriMidine
Tetraethyl aMMoniuM chloride
5-BENZOYLPENTANOIC ACID
5-Chloro-2-ethoxybenzeneboronic acid
Tetrakis(diethylamino)tin
N-Carbobenzoxy-DL-leucine
5-Benzylsulfanyl[1,3,4]thiadiazol-2-ylamine
5-HEXYNOIC ACID
1-ADAMANTANEACETONITRILE
Quinoline-5-carboxylic acid
5,6,7,8-Tetrahydro-2-naphthol
2-Methyl-4-phenyl-5-pyriMidinecarboxylic acid
[N-[alpha-[2-(DibutylglycinaMido)phenyl]benzylidene]glycinato]nickel
N-(4-Methoxybenzyl) 4-broMobenzenesulfonaMide
Tri-n-butyltin chloride, 95%, tech.
Pyrocatechol, Reagent
4-Nitrophenyl alpha-D-Glucuronide
3-Hydroxyphenylacetic acid
ethylenediamine dihydroiodide
Etilefrine Hydrochloride
4-(trifluoromethyl)phenacyl bromide
BERYLLIUM OXIDE
2,7-DibroMonaphthalene
Maleic acid, 98+%
Ethyl 9-fluorenylideneacetate, 98%
Methyl 2-(2,2,2-triMethylacetaMido)nicotinate
1,3-Bis(2,4,6-triMethylphenyl)-1,3-dihydro-2H-iMidazol-2-yli
POTASSIUM TETRANITROPALLADATE(II)
3-METHOXYBUTYL 3-MERCAPTOPROPIONATE
PiperidiniuM PentaMethylenedithiocarbaMate , 95%
CHLORANILIC ACID SODIUM SALT
1-BOC-3-Methylpyrazole-5-boronic acid
Bis(2,4-pentanedionato)titanium(IV) Oxide
3-Mercapto-1-propanol
2,3:4,5-Di-O-isopropylidene-β-D-fructopyranoside sulfamate
2'-Deoxyuridine-5'-triphosphate disodium salt
1-NAPHTHOL-4-SULFONIC ACID SODIUM SALT
DECAFLUOROBENZOPHENONE
Methimazole
Pinacryptol yellow;6-Ethoxy-1-Methyl-2-(3-nitrostyryl)quinoliniuM Methyl salfate;
SodiuM cyclohexanebutyrate
1-BroMo-2,4-bis(trifluoroMethyl)benzene
4-(2-Hydroxy-3-(Morpholino)propoxy)phenylboronic acid, pinacol ester
DL-3-Octanol, 97%
3,5-DiMethyl-4-isoxazolylboronic acid, 97%, May contain varying aMounts of anhydride
4-(4-tert-Butylphenyl)benzoic Acid
4,4'-Di(1,2,3-triazolyl) Disulfide Hydrate
4-Phenoxybenzenesulphonyl chloride
4-trifluoromethyl-3-chloro-2-methoxyl pyridine
3-Fluoro-4-methylphenylisothiocyanate
methyl 2-cyanopyridine-4-carboxylate
(+/-)-HYDROBENZOIN
1,7-Heptanediol
4-(4-BOC--1-carbonyl)phenylboronic acid
Triethyl orthobutyrate

Doxorubicin hydrochloride
Bis(trimethylenedithio)tetrathiafulvalene [Organic Electronic Material]

Lead(II) tetrafluoroborate
3,5-Dimethylphenylboronic acid
2-Hydroxypropyl methacrylate
Benzoylacetonitrile
9-BroMofluorene
15-Crown-4 [4-(2,4-Dinitrophenylazo)phenol]
4-((Tetrahydrofuran-2-yl)MethylcarbaMoyl)phenylboronic acid
IsoaMyl acetate, 99+%, pure
2',6'-Dichloroacetophenone, 97%

4'-Hydroxyheptanophenone
sodium hexane-1-sulphonate
4-Methyl-2-pentanol
Fmoc-D-His(Fmoc)-OH
5-Norbornen-2-Yl(Ethyl)Chlorodimethylsilane
Porcine PP (Pepsin) ELISA Kit
Mouse α2-Antiplasmin,α2-AP ELISA Kit
Human EPCA2 ELISA Kit
MCT ELISA Kit
Chicken LH (Luteinizing Hormone) ELISA Kit
Rat TGF-beta-inducible early response gene-1,TIEG1 ELISA Kit
PDH E1 ELISA Kit
Human OTR (Oxytocin Receptor) ELISA Kit
VEGFR-1/Flt1 ELISA Kit
Guinea pig ACE (Angiotensin I Converting Enzyme) ELISA Kit
Murashige & Skoog Medium (1/2 Sucrose)
Glucose Blood Agar
IsobutylaMine
Glycine Hydrochloride
Methyl 5-chloro-2-hydroxybenzoate, 97%
2-Fluoro-N-(2,5-difluorophenyl)benzaMide, 97%
3-Hydroxyphenylacetic acid
Reactive Red Brown K-B3r
Tween® 85
4-Chloro-2-fluorobenzoyl chloride

4'-Fluoropropiophenone
6-Methyl-1,2,3-oxathiazin-4(3H)-one 2,2-dioxide potassium salt
1-BroMo-3,5-Dichlorobenzene
2-Methyl-1-naphthylMagnesiuM broMide, 0.25 M in 2-MeTHF
N-(2,2,2-Trichloroethoxycarbonyloxy)succiniMide, 98+%
1-(TriMethylsilyl)propyne
1,3-BenzenediMethanethiol
4-BroMo-2-hydroxybenzoic acid
2-(4-Boronophenyl)-5,6-dihydro-4H-1,3-oxazine
2-AMINOBENZOTRIAZOLE
4-Hydroxy-3-nitropyridine
3-Methylthiophene-2-carboxylic acid
Isopropanol-d8, 99+ atom % D, for NMR
5-Cyanouracil
Tribehenin