RN-c大鼠皮层神经元细胞系液体培养基 货号：GD-CP139724
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞，说一点自己培养细胞方面的看法：1)培养细胞前，可以看看细胞特点说明书，包括细胞正常生长的状态图、传代、培养基的类型等；2)不同细胞生长周期不同，有的生长很快，比如说MDA-MB-231，传代的时候取离心悬液的十分之一就已经足够了，有的又是特别慢，等的人心焦，BT474就是，传代是一分二，复苏起始的时候两周多才能长满，所以就要在培养细胞的过程中多多摸索了；3)对于娇弱的细胞，就得细心呵护了，胰酶消化不能过久，吹得时候要轻柔，离心时候也要注意转速和时间，每天都要去看一下细胞，肉眼观察培养基状况、显微镜下观察细胞生长状况；4)冻存细胞复苏后第二天Z好更换一次培养基；5)培养箱隔一段时间彻底用酒精棉擦洗一次；6)养细胞的教训：不能偷懒!细胞虐我千百遍，我待细胞如初恋。1)不管买的细胞、惠赠的细胞，刚拿到手赶紧做两件事：检测是否有支原体污染; 迅速扩增冻存；2)发现有污染迅速处理掉，特别是当你养了很多种细胞时; 细菌污染，真菌污染很容易发现，支原体污染的话，细胞会长的慢，买个支原体检测的kit定期检测一下；3)传代细胞日常的值日清洁是必须的，此外还要定期熏蒸；4)细胞的传代一定要规律，保持相同的confluency和传代时间间隔，不要随心所欲或者拖延；5)注意个人卫生。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Methoxy-pyridin-4-ylaMine
L-GlutaMic acid 5-tert-butyl 1-Methyl ester hydrochloride
2-Chloro-3,4-diMethoxybenzyl alcohol, 97%
LithiuM hydroxide
2-CHLORO-6-METHYLTHIOPHENOL
1,6-HEXANEDIAMINE DIHYDROCHLORIDE
4-Aminopyrazolo[3,4-d]pyrimidine
6-Phenylhexanoic acid, 99%
2-Aminoethanethiolsulfuric acid, 99%
1,1-Diphenylhydrazine Hydrochloride
3-Methyl-1H-pyrazol-5-amine
5-ACETYLVALERIC ACID
NIOBIUM(V) CHLORIDE
2-Chloropropene
4-AMino-3-broMopyridine
5-Fluoroquinazolin-4-ol
1-(DiMethylaMino)-3-[2-(3-Methoxyphenethyl)phenoxy]-2-propanol Hydrochloride
3-AMino-2-fluorobenzoic acid
3-(Phenylthio)thiophene, 97%
1,3,5-TRIMETHYLBENZENE
Cholesterol Acetate
Diallyl maleate
4,6-dichloro-5-methylpyrimidin-2-amine
methyl sorbate
1-Acetylindole
2-Formylphenoxyacetic acid
3,4-Dihydroxybenzoic acid
4,5-Difluorophthalic Acid
Methallylcyclopentane, 99%
Propargyl benzoate, 98%
Benzotrifluoride
4-Chlororesorcinol
2-Chloro-6-ethylaMinopyrazine
NeodyMiuM trifluoride
1,1'-BIS(DICYCLOHEXYLPHOSPHINO)FERROCENE
2-AMino-7-broMofluorene
Terephthalaldehyde Bis(diethyl Acetal)
4-Iodobenzoic acid, 98%
3-Amino-2-bromo-6-methoxypyridine ,97%
1-Acetyl-3-indolecarboxaldehyde, 98%
Glycol sulfite
2,3,6-TRIFLUOROBENZYL ALCOHOL
o-Tolunitrile
2-Nitropropane;β-Nitropropane
2-Chlorobenzonitrile
1-Acetyl-2-iMidazolidinone
2-(4-TrifluoroMethylphenyl)pyrrolidine
4-BroMobutyryl chloride, 95%
1-(4-AMino-2-hydroxyphenyl)ethan-1-one, Tech.
L-Tyrosine hydrochloride
1-Acetyl-4-piperidone
3,7-dimethyloct-7-en-1-ol
N-Boc-D/L-Alaninol
3,5-Dinitrobenzyl alcohol
anti-3-oxotricyclo(2.2.1.0(2,6))heptane-7-carboxy
2,3,5,6-Tetrafluoroaniline
alpha,alpha-Diphenyl-4-piperidinoMethanol
MARGAROYL CHLORIDE
1-Benzyloxy-2-Methoxy-4-propenylbenzene
1H,1H,5H-Octafluoropentyl acrylate
2',4'-Dimethoxyacetophenone
2-Amino-5-chloro-3-nitropyridine
4-Formylcinnamic acid, predominantly trans, 98%
TRANS-2-UNDECENAL
3-ACETAMIDOCOUMARIN
2-Phenyltoluene
2,4,5-Trifluorophenylacetic acid
Poly(ethylene glycol Methyl ether)
1-Phenyl-1-pyridin-2-ylMethanaMine dihydrochloride
Guanidine SulfaMate
2-AMinoMethylpyrazine
3-BroMo-5-fluorobenzotrifluoride, 97%
2-Methyl-1-phenyl-2-propanol
N,N'-Diacetylchitobiose
1-chloro-1-fluoroethylene
N-alpha-t-BOC-L-glutamic-gamma-benzyl ester
1-Methylcyclopropene
Brompheniramine hydrogen maleate
Porcine Heat Shock Protein 70,HSP-70 ELISA Kit
Mouse FMS-like tyrosine kinase 3,Flt3 ELISA Kit
Human hnRNP A2/B1 ELISA Kit
Human D2R/DRD2 (Dopamine Receptor D2) ELISA Kit
Monkey Cys-C (Cystatin C) ELISA Kit
IL15 ELISA Kit
TGFβ1 ELISA Kit
Human PR3-ANCA ELISA Kit
PDGFD ELISA Kit
TPS ELISA Kit
Cysteine Heart Blood Agar with antibiotics
Cysteine Heart Blood Agar with antibiotics
LithiuM acetate dihydrate, 99%
trans-N-Boc-4-cyano-L-proline Methyl ester, 97%
4-n-Butyl-4'-cyanobiphenyl, 98%
2-PhenoxythioacetaMide, 97%
Diallyl maleate
Methyl acetimidate hydrochloride
6-Iodoquinoline
2-Naphthylacetic acid
1,4-Diiodobenzene
Cyclopropyl carbinol
Citronellyl Isobutyrate
6-tert-Butyl-1-indanone, 97%
Tri-n-butyltin acetate, 98%
4-AMino-N,N-diethylaniline Monohydrochloride
2′,6′-Dichloroacetophenone
Methylparaben
4-(DiethylaMino)benzaldehyde-1,1-diphenylhydrazone
(±)-cis-Cyclopentane-1,2-dicarboxylic acid
SUCCINAMIDE
2-Chloro-6-fluorobenzyl Cyanide
6-hydroxy-4-methyl-1-benzofuran-3(2H)-one(SALTDATA: FREE)
Inosine 5'-Triphosphate Trisodium Salt Hydrate
4-tert-Butyl-3-nitrobenzeneboronic acid 98%
