clone 9大鼠肝脏细胞系液体培养基 货号：GD-CP130377
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-BroMobenzenesulfonyl chloride, 98+%
2-Nitropropane
(S)-(?)-2-AMino-4-Methyl-1,1-diphenylpentane
4-(4-BroMophenoxy)benzoic acid
4-BiphenylMethyl chloride
2'-Hydroxyacetophenone
2-METHYL-3-HEPTANONE
Phenyl-p-benzoquinone, 95%
4-(Aminomethyl)cyclohexanecarboxylic acid
3-Methacryloxypropylpentamethyldisiloxane(inhibited with MEHQ)
Methyl 2,2-Dimethoxypropionate
Diphenolic acid
1,3,5-Tri-tert-butylbenzene
Potassium L-aspartate
4,4'-DiaMinobenzanilide
4-Hydroxy-3-MethoxyMandelic acid
(R)-(+)-2-(DiphenylMethyl)pyrrolidine
Methyl 5-broMo-4-chloro-2-Methoxybenzoate
VinylMagnesiuM broMide, 1M solution in 2-MeTHF, AcroSeal
SodiuM Taurocholate, Natural
cis-11-Hexadecenal
Ethyl-beta,beta-dimethyl acrylate
2,5-dihydro-2,5-dioxofuran-3-acetic acid
4-Hydroxycoumarin
4-Pyridinecarboxylic acid, 2-fluoro-, methyl ester
3,4,5,6-TETRACHLOROFLUORESCEIN
4-Methylbenzenesulfonic anhydride
PalladiuM(II)-aMMoniuM chloride
Tetra-n-butylaMMoniuM broMide, 98+%
CeriuM(III) 2-ethylhexanoate, 49% in 2-ethylhexanoic acid, Ce 12%
D-Glucose 6-phosphate dipotassiuM salt hydrate
4,4,4-Trifluoro-1-butanol
2-CHLOROETHYL 4-CHLOROPHENYL SULFONE
Pancreatin
Methyl (S)-(-)-3-BroMoisobutyrate , 97%
Boc-Cha-OMe
1,3-Dimethylthiourea
2-({[3-(Trifluoromethyl)phenyl]sulfonyl}amino)propanoic acid
N-Phenyl-p-phenylenediamine monohydrochloride, 99%
4-Iododiphenyl ether
Methyl 4-chlorophenylacetate
3-Nitrobenzenesulfonyl chloride
CALCIUM ALUMINATE
6-BroMohexanoic acid;6-BroMocaproic acid
Hydrogen broMide
Diazo Red RC
Ozagrel Hydrochloride
N-Methyl 5-chloro-2-Methylaniline, HCl
Piperine, 98%
3-(Methylthio)-1-hexanol
Glycine Sulfate
2-ethyl-1,3-thiazole-5-carboxylic acid
Bismuth subnitrate
dimethoxymethylpropyl-Silane
trypsin inhibitor from lima beans
tert-Butyl peroxy-2-ethylhexanoate
(3R)-(-)-1-BENZYL-3-(METHYLAMINO)PYRROLIDINE
SILVER LACTATE
PINACYANOL CHLORIDE
5-Phenyl-2-thienylboronic acid, May contain varying amounts of anhydride, 95%
4-Methyl-2-nitroaniline
2-Chloro-4-phenylphenol
Metacycline hydrochloride, sec. Standard, 98%
METANIL YELLOW
6-BROMO-3-FORMYLCHROMONE
5-ETHYL-2-VINYL PYRIDINE
LITHIUM CYCLOPENTADIENIDE
2-AMinoacetophenone hydrochloride
1-BroMo-2-chloroethane
4,4'-Methylenebis(2,6-di-tert-butylphenol)
H-Chg-oh hcl
2-(Triphenylphosphoranylidene)succinic anhydride, 97%
PERHEXILINE MALEATE SALT
2-Hydroxy-2-azaadaMantane
4-Methoxy-2-indolinone
4-isopropylresorcinol
sophoridine
1H-1,2,4-Triazole-3-thiol
PP13 ELISA Kit
Slit2 ELISA Kit
Human Pg (Progesterone) ELISA Kit
Human deubiquitinating enzyme,DUB ELISA Kit
Chicken Interleukin 1,IL-1 ELISA Kit
α-SMA ELISA Kit
Chicken TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
Human choline phosphoglyceride,PC/CPG ELISA Kit
Human heme oxygenase 1,HO-1 ELISA Kit
IGF2 ELISA Kit
Blood Agar Base No2
Heart Infusion Agar
Ethyl acetoacetate propylene glycol ketal, 97%
Iron(II) fluoride hydrate, Puratronic|r, 99.99% (Metals basis)
5,6-DiMethoxyindole-2-carboxylic acid, 98%
2-Fluoro-4-(TRIFluoroMETHYL)BenzylaMine
Ethyl-beta,beta-dimethyl acrylate
2-Ethyl-3-hydroxy-6-methylpyridine Hydrochloride
4,4'-Azodibenzoyl Dichloride
3,5-Pyridinedicarboxylic acid
4-Bromo-alpha-phenethylamine
Dextran sulfate sodium
Econazole nitrate
(1S,4S)-(-)-2-Boc-2,5-diazabicyclo[2.2.1]heptane, 97%
Methyl 4-Morpholineacetate, 99%
Hexahydro-1,3,5-triphenyl-1,3,5-triazine, 98%
4-Thiazolecarboxylic acid
Methyl 1-BOC-indole-7-carboxylate
(Perfluorodecyl)ethylene
Carbon-13C Monoxide
2-Toluenesulfonyl isocyanate
NiobiuM hydroxide oxide
3-[(tert-Butoxycarbonyl)amino]isonicotinic acid
Ionomycin Ca2+ salt
2,6-Dimethoxy-4-methylphenol
