A-431[A431] Cell：人皮肤鳞癌细胞系(来源于ATCC等知名细胞库)

A-431[A431] Cell：人皮肤鳞癌细胞系(来源于ATCC等知名细胞库) 货号：GD-C6818999

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
DiethylaluMinuM chloride, 96%, 25% w/w in heptane
AMpicillin Na
(2-CyanoMethoxy)-4-Methoxyphenylboronic acid, pinacol ester
1-[3,5-BIS(TRIFLUOROMETHYL)PHENYL]-2,5-DIMETHYL-1H-PYRROLE
8-Chlorotetrazolo[1,5-a]pyridine
2-CHLOROPHENYL ACETATE
(S)-Boc-3-Methyl-β-Phe-OH
Maltol Isobutyrate
4-(Hydroxymethyl)-1-trityl-1H-imidazole
4,5-Dichlorophthalic Acid
7-amino-4-methyl-2(1H)-quinolinone(SALTDATA: FREE)
Butoconazole
ALUMINUM OXIDE
(R)-3-HYDROXYMETHYL--1-CARBOXYLIC ACID TERT-BUTYL ESTER
Ethyl 2-hydroxy-4-(trifluoroMethyl)-5-pyriMidinecarboxylate
N-Cyclohexylaniline
9,9-Di-n-octylfluorene
N,N-DiMethyl 3-broMobenzaMide
TitaniuM(IV) isopropoxide, 98+%
Poly-L-lysine HydrobroMide
Janus Blue
4-Hydroxy-2-methylquinoline
4-Chloro-1H-pyrazolo[3,4-d]pyrimidine
dl-homocystine
(2S)-2-amino-4-methyl-pentanoic acid
3-Chloro-4-fluoroacetophenone
(OXYDI-2,1-PHENYLENE)BIS(DIPHENYLPHOSPHINE)
Methyl phenylacetate, 99%
3-(2-BroMoethylaMino)propylaMine dihydrobroMide, 97%
6-Cyanonicotinic acid, 97%
2,4′-DDT solution
ACETOACETIC ACID ISOAMYL ESTER
N-IodoMorpholine hydriodide
PROPYL FORMATE
N-(tert-Butyl)hydroxylaMine acetate
2-(TrifluoroMethoxy)benzyl broMide
4-Chloro-2-fluorobenzaldehyde, 99%
2-[5-(3-Chlorobenzylthio)-1,3,4-thiadiazol-2-ylthio]-N-phenylacetamide
s-Triazolo[4,3-a]quinoline, 99%
2,5-Difluorobenzenethiol
Methyl 2,4-dihydroxybenzoate
Z-ORN-OH
3-BROMOCHLOROBENZENE
POLYETHYLENE GLYCOL TRIMETHYLNONYL ETHER
5-AMino-2-enziMidazolinone
5-BroMo-3-nitrosalicylaldehyde
3-BroMo-5-fluoro-2-propoxyphenylboronic acid
6-Hydroxy-2-naphthoic acid, 98%
4-Nitro-2,3-lutidine N-oxide, 97%
(1S,3R)-3-AMinocyclohexanecarboxylic Acid
9-Methylcarbazole
oxalic acid dilithium
5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside
fmoc-D-valine
(Z)-14-Methylhexadec-8-enal
2-FLUORO-5-METHYLPHENOL
4,4'-Bis(2,2-diphenylvinyl)-1,1'-biphenyl
Z-GLU-OME
4-(2,4-DICHLOROPHENYL)BUT-3-EN-2-ONE
1-Fluorohexane 99%
tri-n-Butylfluorotin
mono-Ethyl malonate, 95%
6-Amino-2,3-dihydro-2,2,3,3-tetrafluoro-1,4-benzodioxine 98%
3-HYDROXY-3-METHYL-2-BUTANONE
(4R,5R)-2,2-DIMETHYL-1,3-DIOXOLANE-4,5-DICARBOXYLIC ACID DIMETHYL ESTER
4-Ethylbenzonitrile
Ammonium fluoborate
N,N-Diethyl-1,3-propanediaMine
1,4-Butanediol diMethacrylate
Lipoxidase froM Soybean
6-BroMo-2-quinolinaMine
(2S,5S)-1-(2-(1,3-Dioxolan-2-yl)phenyl)-2,5-diethylphospholane, 97%
Isopropyl 2-methoxyethyl 1,4-dihydro-2,6-dimethyl-4-(m-nitrophenyl)-3,5-pyridinedicarboxylate
Decaethylene Glycol MonoMethyl Ether
1-Sila-3-cyclopentene
5-Bromo-4-chloro-7H-pyrrolo[2,3-d]pyrimidine
Pigment Red 63:1
Propylvaleroylchloride; 99%
PAI-1 ELISA Kit
Aβ ELISA Kit
Human Centromere Protein,CENP-B ELISA Kit
Human SNX9 ELISA Kit
PTH ELISA Kit
P-gp ELISA Kit
Chicken Interferon γ ,IFN-γ ELISA Kit
Human Thyroid Stimulating Hormone,TSH ELISA Kit
Human ANG III (Angiotensin III) ELISA Kit
FGL2 ELISA Kit
Iron Milk Medium
Candida BCG Agar / Candida Bromcresol Green Agar
Isoquinoline-5-Carbaldehyde
LithiuM perchlorate trihydrate, ACS, 63.0-68.0% LiClO{4}
5-Chlorosalicylaldehyde, 98%
2-Fluorocyclohexanone, 96%
4-Hydroxy-2-methylquinoline
4-iodo-2,6-dimethylphenol
Bromine Trifluoride
10-fluorodecan-1-ol

3-AMINOBIPHENYL
5-Methyl-1H-benzotriazole
3,5-Dichloronitrobenzene
Tetra-n-butylaMMoniuM iodotetrachloride, 95%
EthanolaMine benzoate hydrochloride, 98+%
5(6)-Carboxyfluorescein diacetate
Etidronic acid Monohydrate
3-BroMo-2-iodoquinoline
4-Chlorovalerophenone
OCTAETHYLENE GLYCOL MONOMETHYL ETHER
2,5-Difluorotoluene
5,5-Dimethyl-1-pyrroline N-Oxide
2-Acetyl-4,5-dimethylthiazole
3,4-Diaminobenzohydrazide
3-O-Methyl-D-glucopyranose, 99+%