HNE2 Cell：人鼻咽癌复苏贴壁细胞系 货号：GD-C6420525
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(1-Boc-4-piperidinyloxy)acetic acid, 95%
ThiaMethoxaM
3-Methoxycarbonyl-5-(cyclopropylMethoxy)phenylboronic acid, pinacol ester
2-BroMo-6-fluorobenzaldehyde
(S)-(-)-1,2-PROPANEDIOL DI-P-TOSYLATE
2-METHYL-1-HEXEN-3-YNE
4-(1H-1,2,4-Triazol-1-yl)phenol , 97%
Methyl 4-amino-2,3-difluoro-5-nitrobenzoate
4-(N-Benzylsulphonamido)benzeneboronic acid 95%
1-Hexyl-2,3-dimethylimidazolium chloride, 97%
Nonacosanoic Acid
LITHIUM SALICYLATE
IMINODIACETIC ACID DISODIUM SALT HYDRATE
Isopropenyl acetate
HexaaMMinecobalt(III) chloride
SodiuM tetraborate decahydrate
1,8-Diiodoanthracene
3-BroMo-5-trifluoroMethylbenzaldehyde oxiMe
3,5-DiMethyl-4-isoxazolecarbaldehyde, 97%
TriaMcinolone Acetonide, Powder, USP
1,5-DibroMopentane
[1,1'-biphenyl]-4,4'-dicarboxylic acid
2-aminocyanoacetamide
Vat Orange 1
trans-Pterostilbene
PICEATANNOL
alpha-Naphtholphthalein
3,5-Dinitrobenzoic Acid
Copper(II) 2-ethylhexanoate
5-Cyanopyridine-2-carboxylic acid, 95%
D-Biotin
Ethyl Difluoroacetate
2-DODECANOL
N-Methyl-4-pyridinaMine
4-Benzyloxy-3-Methoxyphenylboronic acid
(S)-(+)-2-Heptyl isothiocyanate, 98%
(-)-Menthyl Acetate
2-(4-Methylphenyl)quinoline-4-carboxylic acid
4-(4-Dimethylaminophenylazo)benzoic acid sodium salt, pure, 98%
4,4'-(2-Hydroxybenzylidene)bis(2,3,6-trimethylphenol)
1-(CYCLOHEXYLMETHYL)
2-Chloroethyl carbamate
1,2-DICHLORO-1,2-DIFLUOROETHYLENE
Benzeneacetonitrile8;Benzyl cyanide;ω-Cyanotoluene;α-Tolunitrile;
1,1-Cyclopropanedicarboxylic acid
2-Chloro-L-phenylalanine
3-MethoxyMethylindole
(2E)-3-(DiMethylaMino)-1-(2-pyridyl)-2-propen-1-one
2-Ethylbenzoic acid, 97%
1,8-Diaminooctane
4-(DiethylaMino)benzaldehyde Diphenylhydrazone
Zirconium Oxychloride Octahydrate
1,10-Phenanthroline-4-carboxylic acid
2-Methoxyethyl cyanoacetate
5-methoxyindole-2-carboxylic acid ethyl ester
HOMOPHTHALIC ANHYDRIDE
1,1,1,3,5,5,5-Heptafluoropentane-2,4-dione, 97%
BTB
3-CYCLOHEXENE-1-CARBOXYLIC ACID METHYL ESTER
Trifluoroacetic acid silver salt
1-benzyl-4-bromo-1H-pyrazole(SALTDATA: FREE)
Methyl 3-Phenylpropionate
1H-Pyrrolo[2,3-b]pyridine-5-carbaldehyde
2-Methoxypyrimidine-5-boronic acid
2-Hydroxy-4-methyl-3-nitropyridine
4'-Hydroxy-4-biphenylcarbonitrile
2,4-DiMethylhexane;EthylisobutylMethylMethane
LissaMine rhodaMine B sulfonyl chloride
1-EthylpyridiniuM chloride
Neopentyl Glycol Diacrylate (stabilized with MEHQ)
5-MethylMorpholin-3-one
2-Pyrid-4-ylpyriMidine-5-carboxylic acid, 90%
Cobalt(II) carbonate hydroxide
1,3-DibroMoadaMantane
Isoquinoline-4-carbaldehyde
2-(2-aminoethyl)-1-methylpyrrolidine
5-bromo-1-methyl-1,2,4-triazole
trisodium phosphate anhydrous
Surv ELISA Kit
CACNα1F ELISA Kit
Human Hp-IgG ELISA Kit
Human multidrug resistance-associated protein,MRP ELISA Kit
Monkey ApoB100 (Apolipoprotein B100) ELISA Kit
IFNγ ELISA Kit
Chicken CA2 (Carbonic Anhydrase II) ELISA Kit
Human PKCβ1 (Protein Kinase C Beta 1) ELISA Kit
Human platelet activating factor,PAF ELISA Kit
A.FABP ELISA Kit
pH7.4 PBS Broth
Amies Transport Medium with carbon
Vinylboronic Acid Pinacol Ester
Furo[3,2-b]pyridine-2-carboxylic acid, 97%
5-(1-PyrrolidinylMethyl)thiophene-2-boronic acid pinacol ester, 97%
2-Iodobenzoic acid
[1,1'-biphenyl]-4,4'-dicarboxylic acid
silver tetrafluoroborate
Disperse Yellow 82
2,2,4-Trimethylpentane
3-Fluoro-5-(trifluoromethyl)benzoic acid
CHROMIUM NITRIDE
Boric Acid TriMethyl Ester
4-Cyanobenzeneboronic acid pinacol ester, 97%
4-Cyano-4'-ethoxybiphenyl, 97%
SodiuM lignosulfonate
2-Fluoro-3-nitrotoluene
TetrabroMophthalic anhydride
1-Methoxy-1,3-cyclohexadiene
TriMethyl[4-(phenylethynyl)phenyl]silane
Hydroxyethyl tetraMethylpiperidinol
3-Ethyl-4-formylphenylboronic acid
Cyclopentylboronic acid
5-Nitroguaiacol Sodium Salt
4-chloro-6-ethyl-N,N-dimethylpyrimidin-2-amine(SALTDATA: FREE)
