HNE3 Cell：人鼻咽癌复苏贴壁细胞系 货号：GD-C6419631
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Irinotecan hydrochloride
Phenthoate solution
2,4-DIMETHYLPHENYL ISOTHIOCYANATE
Dithioerythritol
Citronellal
2,6-DiMethylbenzyl chloride
N~1~,N~2~-dimethylglycinamide(SALTDATA: FREE)
4-Fluoro-3-methylphenylboronic acid
Boc-N-methyl-L-alanine ,98%
1-[2-(Benzyloxy)-5-bromophenyl]adamantane 98%
ALLYL N-PROPYL SULFIDE
1-(2-ETHOXYPHENYL) HYDROCHLORIDE
GERMANIUM(II) IODIDE
BREDERECK'S REAGENT
4-(DicyanoMethylene)-2-Methyl-6-(p-diMethylaMinostyryl)-4H-pyran
2,3-Dichlorophenylacetic Acid
Ethylene Glycol Bis(4-carboxyphenyl) Ether
Cbz-L-Tyrosine benzyl ester
2,4-DiMethyl-2,6-heptadien-1-ol, 96%, Mixture of isoMers
BROMODICHLOROACETIC ACID
Glycyl-DL-norvaline
2,6-Dimethylpyridin-3-ol
Disodium Phosphate heptahydrate
Benzyldimethylchlorosilane
8-Nitroquinoline
2,2'-Furoin
4-Aminophenylacetonitrile
PotassiuM Tert-Butoxide
LithiuM 2-ethylhexanoate
3-Methyl-2-nitrobenzoic acid, 98%
Dithiouracil
trans-9-Octadecenoic Methyl ester
Levofloxacin hydrochloride
N,N-DiMethylisopropylaMine , 99%
SodiuM 1-butanesulfonate
ETHYL 5,6-DIMETHYL-1H-BENZIMIDAZOL-1-YL)ACETATE
Methyl 2H-perfluoro-2-methylpropanoate 98%
Tri-n-octylsilane
4-(1-Boc-Piperidin-3-yl)-butyric acid
3,5-Dibromo-2-chloro-pyridine
2,6-DICHLORO-3-FLUOROBENZOIC ACID
1-ETHYLQUINALDINIUM IODIDE
2-FURFURYL ISOTHIOCYANATE
SARCOSINE HYDROCHLORIDE
4-AMino-N,N-diMethyl-3-nitroaniline
2,5-Dithiobiurea
MonoMethyl FuMarate
2-(Methylsulfonyl)-4-(tributylstannyl)thiazole
cis-3-Azabicyclo[3.1.0]hexane-2-carboxylic acid, 95%
N-Cbz-L-Aspartic acid 4-benzyl ester
2-UndecyliMidazole
α-phenylpiperidine-2-acetic acid
(4-tert-butylphenyl)methanamine
Solvent Blue 36
Acetylnitrophenylethylamine
3-Furaldehyde
(R,R)-(-)-N,N'-BIS(3,5-DI-TERT-BUTYLSALICYLIDENE)-1,2-CYCLOHEXANEDIAMINO-MANGANESE(III) CHLORIDE
tert-Butyl N-(2-Hydroxypropyl)carbaMate , 98%
FMoc-Lys(Boc)-OPfp
TAN [=1-(2-Thiazolylazo)-2-naphthol] [Spectrophotometric reagent for transition metals]
(2,4-dioxo-1,3-thiazolidin-5-yl)acetic acid(SALTDATA: FREE)
(R)-2-Isopropylamino-2-phenylethanol
L(-)-Perillaldehyde, 90%
SCHRADAN
Ethyl N-Boc-piperidine-4-carboxylate
2,4,6-Trifluorophenylboronic acid
SodiuM polyacrylate
L-Valinol
2,2′-Methylenebis[(4S)-4-phenyl-2-oxazoline]
4-Sulfocalix[8]arene Hydrate
1-BroMo-2,4-diMethoxybenzene, 98%
(1R,2R)-N,N'-DiMethyl-1,2-cyclohexanediaMine, 95%
REACTIVE VIOLET 5
Tris(triphenylphosphine)rutheniuM(II) Dichloride
3-amino(38C5,38N)pyridine-4...
Reactive Red 180
()-2-allyl-4-hydroxy-3-methylcyclopent-2-en-1-one
Silver p-toluenesulfonate
F10 ELISA Kit
Mouse factor B,BF ELISA Kit
Human GCGR ELISA Kit
Human ETFα ELISA Kit
ATF2 ELISA Kit
IL－4 ELISA Kit
IL6 ELISA Kit
HDV-Ag ELISA Kit
Human NOSTRIN ELISA Kit
8-OHdG ELISA Kit
Cellulose Decomposing Microorganisms Medium
Leptospira Medium Base EMJH
Ethyl 4,6-dihydroxy-5-nitronicotinate, 97%
Trans-4-Cyano-4-(4-n-Pentylcyclohexyl)Biphenyl
4-AcetaMido-1-(3-chloro-2-pyridyl)piperidine, 95%
2-AMinoisophthalic Acid
2,6-Dimethylpyridin-3-ol
bis(pentafluorophenyl)oxalate
2-(methylthio)ethyl methacrylate
alpha,alpha-dibromo-4-nitrotoluene
DL-3-Methylvaleric acid
NEUROKININ A
3-Hydroxypyridine-4-carboxylic acid, 98%
4-(Boc-aMino)-2-chloropyridine, 95%
5-Fluoro-2-Methoxybenzyl broMide, 98+%
Benzoic anhydride
CouMarin 314
(R)-2-Hydroxy-4-phenylbutyric acid
3-BroMo-5-chloroanisole
2,4,6-TriMethyl-N-Methylaniline
MANGANESE ANTIMONIDE
N,N,N'-Trimethylethylenediamine, 97%
N,N,N'',N''-Tetrabutyldiethylenetriamine
Trichloroethylene (1mg/ml in Methanol) [for Water Analysis]
Tri-neo-pentylphosphite, min. 90%
