A204细胞:人横纹肌肉瘤复苏细胞系更多折扣 货号:GD-C5920093
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Fluoro-4-(Methylsulfonyl)benzeneboronic acid, 98%
Pectic acid
3-(4-ETHOXYPHENYL)PROPIONIC ACID
Deoxyribonucleic Acid SodiuM Salt (SalMon Milt)
3-(3-BroMophenyl)-5-propyl-1,2,4-oxadiazole
4-DiMethylaMinopyridine
Paraquat dichloride
Trimethylpentafluoroethylsilane
Methyl methacrylate, stabilized, 99%
Tetraphenylmethane
(1R)-1-(3-Fluorophenyl)ethylamine
2-Methyl-3-biphenylmethanol
2-Nitroaniline-4-sulfonic acid
Eucalyptus oil
Methyl 4-chlorocarbonylbenzoate
2,3,4-Trichlorobenzene-1-sulfonyl chloride
3,6-DibroMo-9-phenylcarbazole
2,4-DibroMoiMidazole
TitaniuM(IV) ethoxide, 33-35% TiO2
PepperMint Oil, NF
2,5-Dibenzylidenecyclopentanone
Alizarin Yellow GG
N-A-benzoyl-L-histidine crystalline
1-(Diphenylmethyl)
p-Methylaminophenol
1-(2-Thienyl)-1-propanone
Titanocene dichloride
GadoliniuM(III) hydroxide hydrate, 99.9% (REO)
Methyl benzo[b]thiophene-2-carboxylate, 98%
2,5-Difluorophenylacetonitrile, 97%
4-(benzyloxy)-2-fluorophenylboronic acid
ForMaldehyde SodiuM Bisulfite HeMihydrate
4′-Hydroxy-3′,5′-diMethylacetophenone
RONGALITE
4-Methyl-1-tetralone
2-(2-PYRIDYL)BENZIMIDAZOLE
4-(4-Ethylphenyl)-4-oxobutanoic acid, 97%
(4-methyl-1,3-thiazol-5-yl)methanol(SALTDATA: FREE)
5-Methyl-2-(4-nitrophenyl)-2H-pyrazol-3-ylamine
2,4-dibromobenzathiazole
Pigment Red 13
Pyrithiobac-sodium
Mebendazole
Moxifloxacin Related CoMpound A;1-cyclopropyl-6,8-difluoro-7-[(4aS,7aS)-octahydro-6H-pyrrolo[3,4-b]pyridin-6-yl]-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
1-BroMohexadecane
Benzoyl Leuco Methylene Blue
2-(4-Boc-piperazin-1yl)Methylphenylboronic acid, pinacol
5,10,15,20-Tetrakis(pentafluorophenyl)-21H,23H-porphine, 95%
4,4'-Difluorobiphenyl, 99%
Todralazine hydrochloride
4-Chloro-6,7-diMethoxyquinazoline
2,4,5-Triphenylimidazole
2-Chloro-L-phenylalanine
Methyl 4-methylthiazole-5-carboxylate
2-Methoxypyrimidin-5-amine
2-Fluorophenylboronic acid
Acetic acid-18O2
AllylaMine Hydrochloride
(9-Fluorenyl)triphenylphosphoniuM broMide, 98%
L-(+)-Lyxose
Di-tert-dodecyl Disulfide (mixture of isomers)
5-Fluoropyrimidine
2-piperidin-4-yl-1,2,3,4-tetrahydroisoquinoline(SALTDATA: 2HCl)
1-TERT-BUTYL-3-ETHYLBENZENE
2-(2-Bromoethoxy)anisole
1-BROMO-2-CHLORO-1,1,2-TRIFLUORO-3-BUTENE
BARIUM PERCHLORATE TRIHYDRATE
Thionin acetate
1,3-Butanediol diacrylate
Benzyl Laurate
2-Hydrazinyl-5-trifluoroMethylpyridine
2,5-DiMethoxy-2-Methyl-2,5-dihydrofuran, 97%
6-Aminopenicillanic acid
4-Nitrophenyl beta-D-Glucuronide [Substrate for beta-Glucuronidase]
(4-acetylphenyl)acetic acid
Machette
Ethanephosphonic acid
1,3-Bis-(dichloromethyl)-1,1,3,3-tetramethyldisiloxane
Zn-MT ELISA Kit
Mouse β-Lactamase inhibitors,BLI ELISA Kit
LPA ELISA Kit
BAIgG ELISA Kit
Chicken Immunoglobulin E,IgE ELISA Kit
Rat L-Selectin ELISA Kit
Chicken Hydroxycorticosteroids,17-OHCS ELISA Kit
Human Estradiol receptor,ER ELISA Kit
Human VIP ELISA Kit
ASPN ELISA Kit
Dextrose Acetate medium
Pseudomonas Cepacia Agar Base
Methyl isonicotinate, 98%
GRAM'S IODINE
5-AMino-2-Methylpyridine
2-Hexanone, 98%
Alizarin Yellow GG
1,2-Cyclohexanedicarboxylic Acid Diethyl Ester
Pigment Red 48:1
2,4-Diaminopyrimidine
2,3,3-TRIMETHYL-1-BUTENE
SUCCINAMIDE
Methyl diethylphosphonoacetate
N^a-FMoc-N^d-trityl-L-glutaMine, 98%
Bis(2-Methoxyethoxy)Methane, 98%
PotassiuM hydrogen diiodate
2-Hydroxy-5-Methylpyridine
2-AcetaMido-5-broMo-3-nitropyridine
6-HEPTEN-1-OL
4-Chloropyridine N-oxide
ZINC TRIFLUOROACETATE HYDRATE
2,4,6-Trimethoxyaniline , 97%
Guanylyl(2′→5′)adenosine ammonium salt ,98%
2,5-Dimethylbenzoic acid, 98%
Citalopram