Neuro-2a[N2a]细胞：小鼠脑神经瘤复苏快细胞系

Neuro-2a[N2a]细胞：小鼠脑神经瘤复苏快细胞系 货号：GD-C4720043
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-Chloro-2(3H)-benzoxazolone, 99%
Ethyl 6-BroMopyridine-2-carboxylate
p,α,α-TriMethylbenzyl alcohol
2,4,6-TRIS(PERFLUORONONYL)-S-TRIAZINE
1,4-Diiodo-2,5-dioctylbenzene
METHYLENECYCLOPENTANE
DIMETHYL 2-(4-FLUOROBENZYL)MALONATE
4-(5-Formylpyridin-2-yl), N1-BOC protected 97%
1-Fluoro-4-(tributylstannyl)benzene 95+%
Bis(dichlorophosphino)methane, min. 90%
4-(Heptyloxy)benzeneboronic acid
8-Bromoquinoline
4-METHOXY-1-NAPHTHOL
2-Acetyl-1-ethylpyrrole
3,4-DIMETHOXYBENZOYL CHLORIDE
Monobutyltin oxide
Dibenzo-15-crown 5-Ether
N-Cyclohexyl 3-broMo-5-nitrobenzaMide
TetrapropylaMMoniuM perruthenate
Octocrylene
4-Butoxybenzoic Acid
16-epiestriol
ethyl 4-Aminocinnamate
1-ethynyl-2-fluorobenzene
1,3-Dihydroxynaphthalene
Dodecyl gallate
4'-DIMETHYLAMINOACETOPHENONE
2-Fluoro-6-Iodoanisole
3,4,5-TriMethoxybenzylaMine, 96%
N-Boc-trans-4-hydroxy-L-prolinol, 96%
D-Ala-D-Ala
2,4-DiMethylphenol Standard
3-BroMo-5-hydroxyphenylboronic acid, pinacol ester
sec-Butyl Methyl Ether , 98%
4-Nitrobenzeneethanol
4-NitrophenethylaMine hydrochloride
(S)-4-(2-Hydroxyethyl)-2,2-dimethyl-1,3-dioxolane
2-methyl-2-(4-nitrophenoxy)propanoic acid(SALTDATA: FREE)
1-(4-Fluorophenyl)-2-methylpropan-1-one
Trierucin
CYCLOPENTADECANE
SOLASODINE
2,4-Dimethoxybenzeneboronic acid
Chloromethyl pivalate
1-Methyl-1H-imidazole-2-carboxylic acid
D-phenylglycine Methyl ester ·HCl
3-(TrifluoroMethyl)phenyltriMethylaMMoniuM Iodide
2,3-DiaMinonaphthalene, 97%
4-Isocyanatobenzoyl chloride, 98%
PHOSPHATE BUFFER
Ethyl Phenylpropiolate
guanosine 5'-triphosphate lithium*prepared enzyma
2-Chloro-4-(trifluoromethyl)pyridine
Benzene, 1-(1,1-dimethylethyl)-2-methoxy-
Silver sulfate
4,4'-SULFONYLDIPHENOL
Boc-Orn(Alloc)-OH
4-(BENZENESULFONYL)THIOPHENE-2-SULFONYL CHLORIDE
SCOPOLAMINE METHYL NITRATE
Pyrocatechol Violet
Tamoxifen citrate, 98%
2,3-Dichloroprop-1-ene 97%
(3-chlorophenyl)(pyridin-3-yl)methanone(SALTDATA: FREE)
3,4-DIAMINOBENZHYDRAZIDE
3-BROMOPHENETOLE
(S)-(-)-2-Bromosuccinic acid
AMpicillin sodiuM salt;
L-Methionine
1,3,5-Tris(diphenylaMino)benzene
N-(TrifluoroMethanesulfonyl)trifluoroacetaMide
2-Ethoxyphenol, 98%
Ethyl 2-Methylphenylacetate, 96%
Sodium 2-propylpentanoate
2-Fluoro-5-Methylpyridine
3',6'-(Dimethylamino)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H...
4-Bromo-2-methoxypyridine
ethyl 3-(2-methylphenyl)-3-oxopropanoate
4-Benzylthiomorpholine 1,1-Dioxide
AGTR2 ELISA Kit
γ IFN-Ab ELISA Kit
Human FASN ELISA Kit
Human CAPN13 ELISA Kit
Chicken NT-3 (Neurotrophin-3) ELISA Kit
COL2α1 ELISA Kit
C3 ELISA Kit
Human FRS3 (Fibroblast Growth Factor Receptor Substrate 3) ELISA Kit
Human Thymopentin,TP-5 ELISA Kit
ApoTf ELISA Kit
Potato Sucrose Water
Charcoal Yeast Extract Agar(Legionella CYE Agar Ba
Ethyl indole-3-acetate, 98+%
Ethyl hydrogen piMelate, 98%
DiMethyl 5-aMinoisophthalate, 98%
trans-2-Methyl-2-pentenoic acid
16-epiestriol
Sodium Phosphate Tribasic Dodecahydrate
4-(chlorophenylmethyl)-1,1'-biphenyl
Citronellyl acetate;3,7-Dimethyl-6-octen-1-yl acetate

4-Bromomethyl-3-nitrobenzoic acid
4,4'-METHYLENEBIS(2,5-DIMETHYLPHENOL)
Mercury(II) TrifluoroMethanesulfonate
N-(4-Chlorobenzyl)-4-fluorobenzylaMine, 97%
4-AMinosalicylic acid, 98+%
2-(1,3,3-TriMethylindolin-2-ylidene)acetaldehyde
DodecylethyldiMethylaMMoniuM broMide
BERYLLIUM OXIDE
4-BENZYLOXYBENZOIC ACID
Octadecanophenone , 95%
4-Chloro-2-nitroanisole, 99+%
1-(5-Methyl-3-phenyl-4-isoxazolyl)-1-ethanone
Sodium Sulfamate
(1S,2S,4S,5S)-2,5-Bis(3,5-di-tert-butyl-2-hydroxybenzylideneamino)bicyclo[2.2.1]heptane
(S)-?-(m-Tolyl)alanine