RMC细胞：大鼠肾系膜传代细胞系 货号：GD-C4619159
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Nitrobenzotrifluoride, 97%
Mebhydrolin napadisylate
Iron(III) 2-ethylhexanoate diisopropoxide, 10% w/v in isopropanol
1,3-Dichloro-7-Methylisoquinoline
Olivetol
trans-4-Methoxy-3-buten-2-one
2-(2'-Hydroxy-3'-sec-butyl-5'-tert-butylphenyl)benzotriazole
N-(4-Bromophenyl)maleimide
2,6-Difluorobenzyl alcohol 98%
Bis(i-propylcyclopentadienyl)zirconium dichloride, 98%
3-(Cyclohexylaminocarbonyl)phenylboronic acid
4-FLUORO-2-METHYLBENZENESULFONYL CHLORIDE
AMMONIUM 2,4,6-TRINITROPHENOLATE
1,3-DIIODOBENZENE
Isophthaloyl chloride
1-(4-BroMo-2-thienyl)ethan-1-one
Diethyl (4-BroMobenzyl)phosphonate
4-VinylbenzylaMine (stabilized with MEHQ)
Trolox?, 97%
SodiuM Benzoate, Ultrapure
N-n-Octyl-3,4-thiophenedicarboxiMide
Methyl butyrate
D-fructose 1,6-diphosphate dicalcium
1-Vinylimidazole
5-Chloro-3-fluorosalicylaldehyde
SILVER P-TOLUENESULFONATE
2,4-DICHLOROPHENYL ISOTHIOCYANATE
Xanthine
4-BroMo-2-Methoxybenzyl alcohol, 97%
3-Acetylbenzoic acid, 98%
Silvex solution
Phenoxyethyl isobutyrate
2-FLUORO-6-(TRIFLUOROMETHYL)BENZOIC ACID
Phenethyl isocyanate
LithiuM iodate
2-BENZO[1,3]DIOXOL-5-YL-
2,6-Difluoro-3-methylbenzaldehyde
N-[3,5-Bis(trifluoromethyl)benzenesulfonyl]-L-methionine methyl ester
1-Propylpiperidin-4-one
Nalidixic acid
2-[(1S,2S)-1-ethyl-2-bezyloxypropyl]-2,4-dihydro-4-[4-[4-(4-hydroxyphenyl)-1-piperazinyl]phenyl]- 3H-1,2,4-Triazol-3-one,
1-(2-CHLOROPHENYL)IMIDAZOLE
Sodium hydroxide
Hydrofluosilicic acid;Hydrosilicofluoric acid;Fluosilicic acid;Silicofluoric acid;Hexafluorosilicic acid;Fluorosilicic acid;Sand acid;
3,4-Dichlorobenzotrifluoride
Hexane-d14
2-Phenylethyl Methyl Ether
(R)-4-Benzyl-2-oxazolidinone, 99%, >99% ee
N-Methyl-N-(3-nitrobenzyl)aMine, 95%
ACETYLCHOLINE IODIDE
2,2-DiMethyl-4-pentenoic Acid
Bis(2-ethylhexyl) phthalate
(3-chlorophenyl)(phenyl)methanol
4-Hydroxypyridine-2-carboxylic acid
2-ethoxytetrahydrofuran
2-(METHYLTHIO)ETHANOL
PENTAMETHYLPHENYLACETONITRILE
4-Penten-1-ol
SodiuM pyruvate-13C3
Ethyl 4-chloroacetoacetate ,98%
3,4-Dichlorobenzyl Chloride
2-Bromothiophene-3-carbonitrile , 97%
4-Sulfocalix[8]arene Hydrate
1-PENTADECANOL
1,1,1-Trichloroethane
(Z)-2-(2-Tritylaminothiazol-4-yl)-2-methoxyiminoacetic acid
1,2-Diethylbenzene
4-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)-3,6-dihydro-2H-pyran
1-Benzofuran-2-carbonyl chloride
4-Fluorophenetole
4-AMino-3,5-dinitrobenzoic acid
1-(2-Pyridin-3-ylphenyl)MethanaMine dihydrochloride, 97%
Methylparaben
Monoisopropyl FuMarate
3,4-dimethylumbelliferone
Reactive Brown 2
disperse red 1
4'-Hydroxy-3'-methylacetophenone
HCY ELISA Kit
Mouse Toll-like receptor 9,TLR-9 ELISA Kit
Human apoprotein B100,apo-B100 ELISA Kit
DA2 ELISA Kit
Chicken Interleukin 6,IL-6 ELISA Kit
DEFA1 ELISA Kit
HCⅡ ELISA Kit
Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA Kit
VCAM-1 ELISA Kit
PKP1 ELISA Kit
Zobell Marine Agar 2216
Mycoplasma Broth Medium with Arginine
MolybdenuM(V) isopropoxide, 99.6% (M.b.), 5% w/v in isopropanol, packaged under Argon in resealable CheMSeal^t bottles
Pararosaniline hydrochloride, 90+%
5-CHLORO-1,3-BENZODIOXOLE
2-Fluoro-5-carboxybenzeneboronic acid, 96%
Methyl butyrate
Metronidazole benzoate
4-Bromo-3-methylpyridine
4-Pyridinecarboxylic acid, 2-fluoro-, methyl ester
2-PHENYLPROPIONALDEHYDE
2-BroMoethylaMine HydrobroMide
3-Hydroxypyridine, 98%
(S)-(+)-2-AMino-1-propanol, 98%
4-Hydroxyphenylacetone, 98+%
PotassiuM broMate
FMoc-Arg(Pbf)-OPfp
3-FLUORO-4-IODOPYRIDINE
Indoline-7-carboxaldehyde
METHYL 4-N-OCTYLOXYBENZOATE
4-AMINO-2,5-DIFLUOROBENZONITRILE
2-Hydroxy-3-methyl-benzaldehyde
7-Bromo-1H-indazole
3-Fluorodiphenylamine 97%
2-Methyl-1-tetralone, 98%
