Calu-1人肺癌细胞株(货号：GD-C919704)

Calu-1人肺癌细胞株
产品货号：GD-C919704
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
我常看到一个比较复杂的四步消化法，这个挺有意思，我也是次听说，应该是网友自己发展出来的方法，很有参考价值。但我估计这个方法可能对付少数非常怪异的细胞才需要这么复杂的程序。我目前养过的近300株细胞里面，还没遇到这么怪异的。比如Caco2其实是很好消化的细胞，不需要胰酶孵育即可，只是有点成片分布。不要以为成片分布是自己没养好，这个视细胞而定。如果和标准形态不一致，那可能是自己没消化好导致的，但如果消化方法正确，仍然成片分布，甚至完全吹打成单细胞悬液，贴壁后仍然成片分布，这是细胞的特性，是因为贴壁过程中重新聚集了。这个时候你拼了命要去让它均匀分布，你的细胞之后会对你越来越不好；比较难消化的细胞(润洗方法5min还不能消化)，就以colon cancer为例，比如HCT15, LS411和KM12，这样的细胞一般需要用少量胰酶孵育，但也不需要很多，一般100 mm dish，一次Z多加入500ul，就足够了，一般我加300ul。即使这样难消化的细胞，一般不超过5min，即可见细胞成片移动，就应该停止消化。一些正常细胞也会有难消化的时候，比如tsDC细胞，但也只要用少量胰酶孵育，3min左右即可看到成片沙状移动。
4-(TrifluoroMethyl)benzyl alcohol
4,5-Difluoro-2-Methylbenzoic acid
2-Chloro-p-cresol , 97%
2-Methylthio-5-(tributylstannyl)pyriMidine
sec-ButylMagnesiuM BroMide (16% in Tetrahydrofuran, ca. 1Mol/L)
2-Ethoxy-5-nitropyridine
2-Norbornanone
Isopropyl isothiocyanate, 95%
2-(Ethylsulfonyl)ethanol
4-Diazo-N-benzyl-N-ethylaniline Chloride Zinc Chloride
CACOTHELINE
Vitamin K1
2-Fluoroaniline
2-Cyano-3-nitropyridine
Guanylthiourea
SodiuM Triacetoxyborohydride
TetrasodiuM EthylenediaMinetetraacetate Tetrahydrate
2-Chloro-6-hydroxybenzoic acid
5-Chloro-1,3-diMethyl-1H-pyrazole-4-sulfonyl chloride, 97%
DICHLOROFLUOROMETHANE
Isoguanine
Pentamethyldisiloxane
beta-Amylase
Colloidal Bismuth Pectin
3-Hydroxybenzyl alcohol
1-Bromo-4-chlorobutane
Cyhalothrin
2-BroMo-7-Hydroxynaphthalene
Ethyl 3-chloro-5-(trifluoroMethyl)pyridine-2-carboxylate, 96%
2,4,6-TriMethylbenzyl alcohol, 98+%
Methylglycol chitosan
Corazonin
4-Hydroxy-2(5H)-furanone
N,N'-(METHYLENEDICYCLOHEXYL)BIS[4-(2-PYRIDYL)-1-PIPERAZINECARBOXAMIDE]
2,3-DIPHENYL-5-(2-THIENYL)TETRAZOLIUM CHLORIDE
4-METHOXYPHENYL 3-O-BENZYL-BETA-D-GLUCOPYRANOSIDE
Triethoxysilylundecanal, tech -95
(4-Fluorophenylthio)propan-2-one
5-(Trifluoromethoxy)indole-2-carboxylic acid
N,N'-Bis(salicylidene)-1,3-propanediamine
Potassium dimethyldithiocarbamate
4'-CHLORO-BIPHENYL-4-YLAMINE
Methoxyethyl oleate
2-Fluoro-6-methylpyridine
5-Pyridin-2-ylthiophene-2-carbaldehyde
2-Phenylquinoline
4,4'-(2-Ethylhexylidene)diphenol
N-Acetyl 5-broMo-2-fluoroaniline
3-Methyl-5-phenyl-4-isoxazolecarboxylic acid, 97%
ENDOSULFAN SULFATE
Didodecyl Succinate
HISPIDULIN
pepsin
1,3,5-Cyclohexanetriol
pentacosane
DIAMMINETETRACHLOROTITANATE (IV)
N-NONACOSANE
N-(4-Methoxybenzyl) 5-broMopicolinaMide
4-BroMo-2-chloro-6-Methylaniline
5-Bromo-2-chlorobenzotrifluoride
3-Cyano-4-fluorophenylboronic acid
6-Hydroxy-1-naphthoic Acid
Fmoc-D-Asu-OH
2-Chloropropionylglycine
Fura 2-AM
ERBIUM ISOPROPOXIDE
Irbesartan Related CoMpound A;1-pentanoylaMino-cyclopentanecarboxylic acid [2'-(1H-tetrazol-5-yl)-biphenyl-4-ylMethyl]-aMide
Hydriodic acid
2,3,4,6-Tetra-O-benzoyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling]
1-BOC-6-BroMo-indole-2-boronic acid
4-Nitrophenyl chloroforMate, 97%
4-Ethoxyphenylacetic acid, 98%
Chloroacetaldehyde diethyl acetal
2-Fluoro-4-Methylpyridine
Alpha,Alpha,Alpha-Trifluoroacetophenone
Ethyl 4-methyl-2-[4-(trifluoromethyl)phenyl]thiazole-5-carboxylate
1,3-Benzenedicarboxylic acid, 4-hydroxy-, 1-methyl ester
[diethoxy(methyl)silyl]methanethiol
Porcine endothelin 1,ET-1 ELISA Kit
AGRP ELISA Kit
Human IGF-ⅠR ELISA Kit
Human DF-Ab ELISA Kit
FAS ELISA Kit
Rat CD97 (Cluster of Differentiation 97) ELISA Kit
IGF2 ELISA Kit
Human PON1 ELISA Kit
Human IGF-R1 ELISA Kit
PⅢNP ELISA Kit
Gause’s Synthetic Broth Medium,Modified
CN Agar Base
Glyoxal diMethyl acetal, 60% aq. soln.
trans-1-n-Pentyl-4-(4-n-propylcyclohexyl)benzene, 99%
4-Ethylbenzoic acid, 99%
2-AMinobenzaMidoxiMe, 97%
Pentamethyldisiloxane
2-Chloro-5-methylthiophene
tris[2,2'-bipyridine]ruthenium dichloride
Tetraphenylethylene

5-Iodo-2-furancarboxaldehyde
NICKEL NITRATE
Methyl 3-AMinothiophene-2-Carboxylate
4-BroMo-2,3,5,6-tetrafluorobenzotrifluoride, 99%
1-Phenylnaphthalene, 97%
2-Fluoro-5-iodobenzaldehyde
TetraMethylaMMoniuM nitrate
N-Octyl-D-glucaMine
N,N,3,5-TETRAMETHYLANILINE
4-TERT-BUTYLBENZYL ALCOHOL
(+)-CaMphene
3-(Cyclopentyloxy)-4-methoxybenzaldehyde, 97%
2-(BOC-amino)ethyl bromide, 98%
3-Methyl-5-isoxazoleacetic acid ,98%
Dimethyl 4-chloropyridine-2,6-dicarboxylate ,97%