特别提示:包括小鼠抗a-SMA抗体(α-平滑肌肌动蛋白抗体)在内,本公司的所有产品仅可用于科研实验,严禁用于临床YL及其他非科研用途!
产品名称:小鼠抗a-SMA抗体(α-平滑肌肌动蛋白抗体)
英文名称:α-Smooth Muscle Actin Rabbit Polyclonal Antibody
产品规格:50μl
本品为来源于兔的抗α-平滑肌肌动蛋白抗体的多克隆抗体
宿主:兔
用途:WB,ICC, IHC,IF
交叉反应性:人,小鼠,大鼠,鸡,牛,马,猴
分子量:42KDa
推荐稀释比例:WB(1:1000),IF(1:100),IHC(1:100),ICC(1:100)
克隆性:多克隆抗体
About this Antibody |
Name | α-Smooth Muscle Actin Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of smooth muscle α-actin. |
Gene ID | 59 |
SwissProt | P62736 |
Synonyms | AAT6; ACTSA; MYMY5; Alpha smooth muscle Actin |
Category | - |
Background | Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis. |
注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果,请考虑使用上述检测专用的一抗稀释液。
储存条件:-20℃,有效期一年。