描述Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.
This Opti-MEM® I is modified as follows: With |
• L-glutamine |
• Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM® I MediumOpti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO
2 environment to maintain physiological pH.
cGMP manufacturing and quality systemOpti-MEM® I is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Grand Island facility (31985-070). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standards.
规格
Glutamine: | L-Glutamine |
Phenol Red Indicator: | Phenol Red |
Form: | Liquid |
Product Line: | Gibco®, Opti-MEM® |
Product Size: | 100 mL |
Volume (Metric): | 100 ml |
Green Features: | Sustainable packaging |
Shipping Condition: | Room Temperature |
内容及储存Storage conditions: 2-8°C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 18 months from date of manufacture.