特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
北京百奥莱博专业生产销*(代"售")核酸外切酶I 工具酶,我公司供应的分子生物学试剂品类齐全,且具有优势价格,欢迎新老客户垂询订购。
名称:核酸外切酶I 工具酶
编号:JN0071
规格:750U
英文名:Exonuclease I
品Pai:百奥莱博
产地:北京
本酶是单链特异性3"→5"核酸外切酶,从ssDNA的3"-OH末端分解生成5"-单核苷酸。对单链DNA的特异性非常高,不分解双链DNA及RNA。可通过80℃,15分钟的热处理使之失活。本制品是把Exo I基因(E. coli)在大肠杆菌中进行表达后,经多次纯化分离而得到的。
产品用途:
1、酶解法清洁PCR反应产物。
2、如需对PCR产物进行测序,需去除反应体系中残存的引物及dNTP,否则测序反应则不能正常进行。外切核酸酶I可用来去除残留的单链引物以及扩增反应中产生的多余的单链DNA。虾碱性磷酸酶(SAP)用来去除残存的单核苷酸(dNTP),之后不需要对PCR产物进行纯化,即可以进行测序反应。此法可以避免繁琐的胶回收、柱纯化、沉淀、磁珠、过滤、透析等方法。
3、在反应液中去除ssDNA fragment。
活性定义:以热变性小牛胸腺DNA为底物,在37℃、pH9.5的条件下,30分钟内产生10 nmol的酸可溶性物质所需要的酶量定义为1个活性单位(U)。
质量保证:经多次柱纯化,SDS-PAGE胶检测仅可见清晰单一的目的条带;PCR方法检测无大肠杆菌DNA残留,无核酸内、外切酶污染。
热失活:65℃,20分钟。
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我公司的核酸外切酶I 工具酶,性价比高,货期短,送货上门,欢迎您来电咨询购买,另外,我公司还生产供应销*(代"售")下列产品:
HR0941 MPO活性检测试剂盒 MPO activity assay kit
WE0248 DNA快速连接试剂盒 Quick DNA Ligation Kit
SV0734 StuI限制性内切酶 StuI Restriction Endonuclease
SY0584 细胞膜深红色荧光探针 DiR Iodide (DiIC18(7))
KFS338 细胞毒性(死细胞)荧光检测试剂盒(FM、HCS法)
YT259 STAT5凝胶迁移探针(10μM) STAT5 Probe For EMSA(10μM)
SV1473 T7 表达 lysY/lq E. coli 感受态细胞 ( GX级 )
BTN80202 PAGE胶DNA柱式回收试剂盒 DNA column recovery kit for PAGE gel
BTN130641 T4核酸内切酶V T4 Endonuclease V
YT914 中性红染色液 Neutral Red Staining Solution
HR0127 固定细胞蛋白提取试剂盒 Fixed cell protein extraction kit
BTN80809 蛋白*(代"磷")酸酶YZ剂混合液Ⅰ Phosphotase Inhibitor Cocktail 1
SV0041 AlwI限制性内切酶 AlwI Restriction Endonuclease
SY0323 蛋白酶YZ剂Cocktail,EDTA-free,迷你型,片剂 Protease Cocktail, EDTA-free, mini, tablet-form
KFS095 10×Tris-CAPS半干转电转移缓冲液
BTN101205 1M Tris-HCl(pH7.5)(DNA级)
SV0965 DNA 聚合酶 I(E. coli)
核酸外切酶I 工具酶关键词:Exonuclease I,核酸外切酶I,JN0071
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YT364 唾液酸酶检测试剂盒(神经*酸酶检测试剂盒) Neuraminidase Assay Kit
SV1624 CLIP-Surface 547
BTN101005 即用型易错PCR试剂盒 Instant Error-Prone PCR Kit
YT157 CFDA SE细胞增殖与示踪检测试剂盒 CFDA SE Cell Proliferation Assay and Tracking Kit
SY0075 KLF4-Luc荧光素酶报告基因质粒 KLF4 luciferase reporter plasmid
HR0334 Lowry蛋白定量试剂盒 Lowry protein quantitative Kit
BTN130899 GAL指示剂培养基 GAL Indicator Medium
SV0310 DraI限制性内切酶 DraI Restriction Endonuclease
SY0437 髓鞘少突胶质细胞糖蛋白(MOG) (35-55) Myelin Oligodendrocyte Glycoprotein(35-55)
KFS198 Caspase 3分光光度法检测试剂盒 Caspase-3 Colorimetric Assay Kit
RFT072 5×Tris-甘*酸-SDS电泳缓冲液
SV1165 ET SSB 极高热稳定单链结合蛋白 SSB ET very high heat stable single chain binding protein
BTN3672 植物DNA提取试剂盒 Plant DNA extraction kit
WE0253 BL21(DE3)感受态细胞 BL21(DE3)Competent Cell
核酸外切酶I 工具酶关键词:Exonuclease I,核酸外切酶I,JN0071
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·重组小鼠*结合蛋白S100B
编号:JN1441
英文名称:Recombinant Mouse S100 Calcium Binding Protein B
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Glu92在C端含有His标签。
S100B质量控制:>95%(还原性SDS-PAGE)
S100B制剂:冻干品
S100B保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
S100B复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于S100B:
S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in Mouse melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
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北京百莱博科技有限公司是工具酶产品的专业生产供应销*(代"售")商,恭候您的咨询选购核酸外切酶I 工具酶。
温馨提示:不可用于临床ZL。