特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
北京百奥莱博专业生产销*(代"售")BL21(DE3)感受态细胞(pTf16分子伴侣)供应,我公司供应的分子生物学试剂品类齐全,且具有优势价格,欢迎新老客户垂询订购。
名称:BL21(DE3)感受态细胞(pTf16分子伴侣)供应
编号:JN0128
规格:100μl×10
英文名:Chaperone competent cells pTf16/BL21(DE3)
品Pai:百奥莱博
产地:北京
外源蛋白质在大肠杆菌中的表达常形成包涵体或发生蛋白酶降解。大多是由于所表达的蛋白未能正确折叠造成。分子伴侣协同作用,可以帮助外源蛋白正确折叠。不同外源蛋白需要的分子伴侣不同。百奥莱博提供5种表达常用分子伴侣的BL21(DE3)衍生菌株,适用于不同外源蛋白在大肠杆菌中有效折叠。该系统适用于pET系列等依赖T7启动子的表达载体。本感受态细胞无污染,转化效率可达107以上
图例一)Human种属的RORG(31KD)在大肠杆菌中获得可溶表达。
Lane 1:上样
Lane 2:流穿
Lane 3: 50mM Imidazole洗脱
Lane 4: 500mM Imidazole洗脱
Lane 5:蛋白Marker
关于BL21(DE3)感受态细胞(pTf16分子伴侣)供应的更详细说明,请致电我公司咨询,我们将以Z饱满的热情为您提供解答,此外,我公司专业供应下列产品:
·重组人细胞色素B(CYB)
编号:JN1991
英文名称:Recombinant Human Cytochrome b5 type A
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Asp134在N端含有His标签。
CYB质量控制:>95%(还原性SDS-PAGE)
CYB制剂:冻干品
CYB保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
CYB复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于CYB:
Cytochrome b5 (CYB) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB can result in type IV hereditary methemoglobinemia.
·重组人TNFRSF5(CD40)
编号:JN0443
英文名称:Recombinant Human CD40L Receptor
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Glu21-Arg193在C端含有His标签。
CD40/TNFRSF5质量控制:>95%(还原性SDS-PAGE)
CD40/TNFRSF5制剂:冻干品
CD40/TNFRSF5保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
CD40/TNFRSF5复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于CD40/TNFRSF5:
CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand (CD40L) leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins (adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
BL21(DE3)感受态细胞(pTf16分子伴侣)供应关键词:JN0128,Chaperone competent cells pTf16/BL21(DE3),BL21(DE3)感受态细胞(pTf16分子伴侣)
KFS058 10×Tris-甘*酸蛋白电泳缓冲液
SY0286 多片段一步法快速克隆试剂盒 Plus Multi One Step Cloning Kit
RFT180 新霉素溶液(50mg/ml) Neomycin solution
BTN111116A 一站式GST标记蛋白质微量纯化套装 One-Stop GST-Tagged Protein Miniprep Pack(A)
HR0053 细菌蛋白提取试剂盒 Bacterial protein extraction kit
YT872 封闭液(用PBS配置,含吐温20) Blocking Buffer(PBS)
YT020 DNA Ladder(200bp-10kb)(21条带)(DNA分子量标准) DNA Ladder (0.2-10 kb, 21 bands)
BTN90602I DNA marker(φX174 DNA/Hinc II) DNA ladder(φX174 DNA/Hinc II)
SV1419 pMAL-c5X 载体
YT222 CREB凝胶迁移突变探针(10μM) CREB Mutation Probe For EMSA(10μM)
KFS304 细胞自噬检测试剂盒(IF/ICC法),双色可选
SY0544 支原体去除试剂2(100X) Antibiotic Solution 2(100X)
SV0619 PstI-HF限制性内切酶 PstI-HF Restriction Endonuclease
WE0211 6×UltraStain上样缓冲液 6×UltraStain Loading Buffer
HR0506 细菌活性检测试剂盒-AlamarBlue Bacterial activity assay kit -AlamarBlue
SY0182 CREB-GFP报告基因质粒 CREB GFP Reporter Plasmid
RFT040 36KD蛋白Marker
BTN130564 大肠杆菌Tuner(DE)plysS感受态细胞 E.coli Tuner(DE)plysS Rosetta Competent Cell
ALH181 鱼类组织DNA提取试剂盒 Fish tissue DNA Extraction Kit
YT511 RNase H Ribonuclease H
BL21(DE3)感受态细胞(pTf16分子伴侣)供应关键词:JN0128,Chaperone competent cells pTf16/BL21(DE3),BL21(DE3)感受态细胞(pTf16分子伴侣)
·重组人GALNT7(N-乙酰*基半乳糖转移酶7)
编号:JN1143
英文名称:Recombinant Human N-acetylgalactosaminyltransferase 7
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Pro30-Val657在C端含有His标签。
GALNT7质量控制:>95%(还原性SDS-PAGE)
GALNT7制剂:液体
GALNT7保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于GALNT7:
N-acetylgalactosaminyltransferase 7(GALNT7) is expressed in uterus, retina, kidney, small intestine, omentum, stomach and CNS.It belongs to the glycosyltransferase 2 family and galNAc-T subfamily. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation.
北京百莱博科技有限公司是克隆与表达产品的专业生产供应销*(代"售")商,恭候您的咨询选购BL21(DE3)感受态细胞(pTf16分子伴侣)供应。
温馨提示:不可用于临床ZL。