特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
北京百奥莱博专业生产销*(代"售")YT686型兔抗β-Catenin抗体(β连环蛋白抗体)库存,我公司供应的抗体抗原品类齐全,且具有优势价格,欢迎新老客户垂询订购。
名称:YT686型兔抗β-Catenin抗体(β连环蛋白抗体)库存
编号:YT686
规格:50μl
英文名:β-Catenin Rabbit Polyclonal Antibody
品Pai:百奥莱博
产地:北京
本品为来源于兔的抗β连环蛋白抗体的多克隆抗体
宿主:兔
用途:WB,IHC,FC
交叉反应性:人小鼠大鼠
分子量:85KDa
推荐稀释比例:WB(1:1000),IHC(1:200),FC(1:50-1:100)
克隆性:多克隆抗体
About this Antibody |
Name | β-Catenin Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to β-catenin. |
Gene ID | 1499 |
SwissProt | P35222 |
Synonyms | CTNNB; DKFZp686D02253; FLJ25606; FLJ37923; β-catenin; Beta-Catenin |
Category | Developmental Biology |
Background | β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. |
注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果,请考虑使用上述检测专用的一抗稀释液。
储存条件:-20℃,有效期一年。
YT686型兔抗β-Catenin抗体(β连环蛋白抗体)库存正在火爆,除此之外,为您推荐下别产品:
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YT686型兔抗β-Catenin抗体(β连环蛋白抗体)库存关键词:YT686,抗体,兔抗,兔抗β-Catenin(β连环蛋白)抗体,β连环蛋白抗体
·琼脂糖
编号:YT019
英文名称:Agarose
规格:50g
本品为不含DNase、RNase和Protease的琼脂糖,配制成的琼脂糖凝胶的凝固点为 34-38℃,熔点为87-89℃。常用于配制核酸分析凝胶,例如用于DNA电泳的琼脂糖凝胶、用于RNA电泳的琼脂糖凝胶等。常使用TAE或TBE作为电泳液。
琼脂糖凝胶的浓度和DNA电泳的分辨率及理想的电泳液参考下表。对于分辨率要求不高时,使用TAE和TBE均可;对于分辨率要求比较高时,对于较低浓度的胶和大分子量核酸宜使用TAE,对于较高浓度的胶和小分子量核酸宜使用TBE。
胶浓度(w/v) | 理想分离范围 | 理想的电泳液 |
0.8% | 800-22,000 bp | TAE |
1.0% | 500-10,000 bp | TAE/TBE |
1.2% | 400-7,000 bp | TAE/TBE |
1.5% | 250-5000 bp | TAE/TBE |
2.0% | 150-3,000 bp | TBE |
储存条件:室温。
YT686型兔抗β-Catenin抗体(β连环蛋白抗体)库存关键词:YT686,抗体,兔抗,兔抗β-Catenin(β连环蛋白)抗体,β连环蛋白抗体
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北京百莱博科技有限公司是一抗产品的专业生产供应销*(代"售")商,恭候您的咨询选购YT686型兔抗β-Catenin抗体(β连环蛋白抗体)库存。