高通量单细胞力学荧光测试分析系统

高通量单细胞力学荧光测试分析系统将细胞生物力学特性同流式细胞仪有机的结合起来，能够在单细胞水平下，高通量、无生物标记物的条件下，快速研究细胞的生物力学特性。

为了给细胞加载力学刺激，单个细胞被泵驱动通过横截面略大于细胞的横截面的微通道。细胞周围的流体的压力梯度创造出一个流动剖面而影响细胞的水动力学。通过流体的流速和粘度可控制作用于细胞上的力。
细胞可以通过水动力可使细胞发生形变；
力由流体的流速和粘度控制；
软细胞能展示出更大程度的变形。

产品配置列表
高通量单细胞力学特性分析模块
包括：
 - 高速成像系统（CMOS相机和图像采集卡）
 - 大功率LED照明系统（460 nm）
 - 带两个注射器模块的精密注射泵
 - 泵架，SampleStage和SampleBox，
 - 高性能计算机
 - 控制和采集软件（单机许可证，附带许可协议）
- 用于后期处理的数据可视化软件
倒置显微镜 - 蔡司Axio Observer 3
和高通量单细胞力学特性分析模块一起进行“实时高通量细胞力学性能测试”。
包含：
 - 左侧端口，与高速相机实现高速通信
 -  40x物镜，NA 0.65，空气/无浸泡
 - 手动XY平台
2.1 附件选项1：升级到自动 XY 平台
2.2附件选项2：升级到 Axio Observer 7 和自动XY 平台
保温模块（选配）
与蔡司 Axio Observer兼容，加热高通量单细胞力学特性分析模块，控制样品在测试期间的温度在室温和37°C之间。
荧光模块（选配）
在单细胞力学测量同时，进行荧光强度测量。
与蔡司Axio Observer显微镜兼容，激光发射模块直接接到显微镜侧面端口上。 
可选择的激发波长和检测通道如下：
3.1 激发波长 488nm，用于FITC，GFP等激发
3.2激发波长 561 nm，用于PE, mCherry等激发
3.3激发波长 640 nm，用于APC, Cy5等激发 
3.4 探测通道 500-550 nm，用于探测 FITC, GFP等
3.5探测通道570-610nm，用于探测PE, mCherry等 
3.6探测通道665-735 nm，用于探测,APC, Cy5等
起始工具包
可用于100个实验，包括：
 -样品注射器，针头，连接管
 -  100 FlicXX（可选择通道尺寸：15,20,30或40μm）
 -  120毫升CellCarrier（可选择粘度：高或低）

代表文献：
[1] High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing. Scientific Reports 2017. Guzniczak E., Mohammad Zadeh m., Dempsey F., Jimenez M., Bock H., Whyte G., Willouy N. & Bridle H.
[2] Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion. BioRxiv. preprint. 2017
Surcel A., Schiffhauer E.S., Thomas D., Zhu Q., DiNapoli K., Herbig M., Otto O., Guck J., Jaffee E., Iglesias P., Anders R., Robinson D.
[3] Real-time fluorescence and deformability cytometry - flow cytometry goes mechanics. BioRxiv. preprint. 2017. Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.
[4] Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood. BioRxiv. preprint. 2017. Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.
[5] Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants. Oxidative Medicine and Cellular Longevity. 2017. Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.
[6] Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties. ACS Biomater. Sci. Eng. 2017. Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.
[7] Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nature Communications. 2017. Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.
[8] High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications. Cytoskeleton. 2017. Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.
[9] Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry arXiv.org. 2017 Herold C.
[10] Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion. PNAS. 2017. Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.
[11] Initiation of acute graft-versus-host disease by angiogenesis. Blood. 2017. Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.
[12] V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy. Oncotarget. 2016. Bartel K, Winzi M, Ulrich M, Koeberle A, Menche D, Werz O, Müller R, Guck J, Vollmar AM, von Schwarzenberg K.
[13] The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512. Mol Metab. 2016. Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.
[14] pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy. eLife 2016. M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev and S. Alberti. A
[15] Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry. Integrative Biology 2016. M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck and O. Otto.
[16] Myosin II Activity Softens Cells in Suspension. Biophysical Journal 2015. C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck and F. Lautenschläger.
[17] Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia. Oncotarget 2015. M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, and U. Oelschlägel.
[18] Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment. Biophysical Journal 2015. A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck and E. Fischer-Friedrich.
[19] Cell Mechanics: Combining Speed with Precision. Biophysical Journal 2015. Hans M. Wyss
[20] Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nature Methods 2015. O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck
[21] Mechanics Meets Medicine Science Translational Medicine 2013. Jochen Guck and Edwin R. Chilvers.