特别提示:包括重组人FGF-acidic在内,本公司的所有产品仅可用于科研实验,严禁用于临床YL及其他非科研用途!
产品名称:重组人FGF-acidic
英文名称:Recombinant Human Acidic Fibroblast Growth Factor
产品货号:GS4383
产品规格:10μg
分子量:Approximately 16.0kDa,a single non-glycosylated polypeptide chain containing 141 amino acids.
储存条件:冷冻(-20℃)
概述
Human aFGF,encoded by the FGF1 gene,is a member of the fibroblast growth factor(FGF)family.Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate.After further fractionating the extract using acidic and basic pH,two different forms have isolated that named "acidic fibroblast growth factor"(FGF-1)and "basic fibroblast growth factor"(FGF-2).Human aFGF shares 54%amino acid sequence identity with bFGF.In mammalian FGF receptor family has 4 members,FGFR1,FGFR2,FGFR3,and FGFR4,and 1,2,3 have 2 sub-types “b”,“c”.aFGF can bind and activate all 7 different FGFRs.Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan.aFGF plays an important role in the regulation of cell survival,cell division,angiogenesis,cell differentiation and cell migration.aFGF are also involved in a variety of biological processes,including embryonic development,morphogenesis,tissue repair,tumor growth and invasion.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观 |
Sterile Filtered White lyophilized(freeze-dried)powder. |
纯度 |
>95%by SDS-PAGE and HPLC analyses. |
来源 |
Escherichia coli. |
生物活性 |
Fully biologically active when compared to standard.The as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5ng/ml,corresponding to a specific activity of>2.0×106 IU/mg in the presence of 10μg/ml of heparin. |
成分 |
Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
内毒素 |
Less than 1 EU/μg of rHuaFGF as determined by LAL method. |
复溶 |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
氨基酸序列 |
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDTDGLLYGSQTPNEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
根据您的关注的
重组人FGF-acidic,FGF-acidic, Human,Recombinant Human Acidic Fibroblast Growth Factor,重组Acidic Fibroblast Growth Factor,重组人Acidic Fibroblast Growth Factor,您可能还对以下产品有需求:
名称:重组小鼠白介素IL-13(白细胞介素13)
货号:JN0393
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备,目的基因编码的Ser26-Phe131表达纯化而来。
IL-13质量控制:>95%(还原性SDS-PAGE)。
IL-13制剂:冻干品
IL-13保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
IL-13复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于IL-13:
Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.
关注
重组人FGF-acidic,FGF-acidic, Human,Recombinant Human Acidic Fibroblast Growth Factor,重组Acidic Fibroblast Growth Factor,重组人Acidic Fibroblast Growth Factor的同时,为您推荐更多您可能感兴趣的产品:
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