ActiveMax® Recombinant Human LIF

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The <0.1 ng/mL, corresponding to a specific activity of >1X107 Unit/mg.

产品背景:
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

产品描述:
rhLIF, with Gly-Pro at the N- terminus, has a calculated MW of 19.9 kDa. The predicted N-terminus is Ser 23. The reducing (R) protein migrates as 33-45 kDa.