mirVana™ PARIS™ Kit, without manual_详细资料

产品信息

描述

mirVana PARIS试剂盒适用于从一份样品中分离天然蛋白质和RNA (包括小RNA)。每个试剂盒均包含足够用于40次蛋白质和总RNA (包括小RNA) 分离或20次蛋白质分离和大、小RNA片段分离的试剂和耗材。

从相同样品分离含小RNA的总RNA和天然蛋白质

样品起始量可为100 – 10⁷个培养细胞或至多100 mg的哺乳动物组织

富集小RNA <200 nt，提高下游分析灵敏度

30分钟的简单操作流程

适合相关的mRNA、miRNA或siRNA和蛋白质水平分析

试剂盒的工作原理

mirVana PARIS试剂盒程序首先采用一种包含非离子型洗涤剂的特殊细胞裂解缓冲液进行样品均质化。由于蛋白质保持完整，因此可取一部分裂解液直接用于常规蛋白质分析应用。剩余裂解液则采用了结合有机和固相提取优势、同时避免二者劣势的操作程序进行RNA的分离。

详细说明

常用规格

Sample Type (General):
Tissue,

Cells

Sample Type (Specific):
Tissue (Mammalian),

Cell Cultures

Final Product:
siRNA,

Transcriptome RNA,

Total RNA,

micro RNA,

Protein (Native),

snRNA

Downstream Application:
Western Blotting,

Northern Blotting,

RT-PCR (Endpoint),

cDNA Library Construction,

microRNA Analysis,

Microarray Analysis,

Real-Time PCR

High Throughput Compatibility:
Manual Protocols,

Not High Throughput-Compatible

Isolation Technology:
Organic Extraction,

Spin Column (Glass Fiber Filter),

Cell Lysis (Nonionic Detergent)

Product Size:
40 preps

Starting Material (Amount):
Up to 10^7 cells,

Up to 100 mg tissue

Shipping Condition:
Room Temperature

Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures.

您可能感兴趣的产品

Sample Type (General):	Tissue, Cells
Sample Type (Specific):	Tissue (Mammalian), Cell Cultures
Final Product:	siRNA, Transcriptome RNA, Total RNA, micro RNA, Protein (Native), snRNA
Downstream Application:	Western Blotting, Northern Blotting, RT-PCR (Endpoint), cDNA Library Construction, microRNA Analysis, Microarray Analysis, Real-Time PCR
High Throughput Compatibility:	Manual Protocols, Not High Throughput-Compatible
Isolation Technology:	Organic Extraction, Spin Column (Glass Fiber Filter), Cell Lysis (Nonionic Detergent)
Product Size:	40 preps
Starting Material (Amount):	Up to 10^7 cells, Up to 100 mg tissue
Shipping Condition:	Room Temperature
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.