美国西格诺“DNA质谱阵列基因分析系统”

新闻报道

2013年3月31日ZY电视台13频道《晚间新闻》报道《我国科学家发现乙肝癌变关键风险基因，为预防并提前干预乙肝的癌变提供了可能》为题的新闻，这一研究成果已在国际权威学术期刊《自然与遗传学》上发表。

这一研究成果是由复旦大学遗传学研究所、遗传工程国家ZD实验室完成的一项重大研究成果，借助美国西格诺公司研发的产品“MassARRAY^®DNA质谱阵列基因分析系统”研究得来。通过这套设备成功的确定了人的STAT4和HLA-DQ基因是乙肝患者罹患肝癌的关键易感基因。

【视频】http://tv.people.com.cn/n/2013/0331/c61600-20978559.html

相关视频图片：

图中设备为西格诺公司产品“MassARRAY^®DNA质谱阵列系统专用自动液体处理仪”，该仪器预先内置反复优化的实验加样程序，适用于DNA质谱阵列基因分析系统的各项应用。

西格诺公司产品“MassARRAY^®微量点样系统”可以快速地将纳升级微量样本转移到SpectroCHIP^®芯片上。

SpectroCHIP^®芯片，第四代提供两种芯片：96孔和384孔基因芯片。也就是说单张芯片可实现96个或者是384个生物样本的多重SNP基因型分析实验，实验成本更低。

图中设备为美国西格诺公司研制的MassARRAY^®主机（三代），现在销售第四代产品。他是一款专为核酸研究而设计制造的台式MALDI-TOF质谱仪，适用于各种核酸检测。

 

他是目前世界上WY应用质谱技术专业检测复杂生物样本中痕量核酸的高精度定性定量技术平台

仪器名称   MassARRAY® DNA质谱阵列基因分析系统
英文名称   MassARRAY Analyzer 4 system
国产/进口 进口
产地/品Pai 美国/西格诺sequenom
型号         MassARRAY

 

功能优势：

中高通量SNP基因分析
GWAS及二代测序验证
肿瘤体细胞基因突变谱分析
基因表达定量及目标基因CNV分析
DNA甲基化定位定量
病原体分子分型

 

应用范围：

生物医学 临床分子诊断 转化医学 肿瘤学 非侵入性产前诊断 个体化YL 微生物流行病学研究 动物遗传育种 农业基因组学研究等

      美国Sequenom公司是世界著名的生物高科技仪器及配套试剂的专业生产企业，总部位于美国亚哥市。公司致力于研发ZX进的MassARRAY? DNA质谱阵列基因分析系统，为生物医学、临床分子诊断、转化医学、肿瘤学、非侵入性产前诊断、个体化YL、微生物流行病学研究、动物遗传育种及农业基因组学研究等提供独特解决方案。

      MassARRAY®DNA质谱阵列基因分析系统能对基因组进行全方位定性和定量研究。该系统WM地整合了PCR技术的高灵敏度、质谱技术的高精确度和芯片技术的高通量，是目前WY应用质谱技术专业检测复杂生物样本中痕量核酸的技术平台。

      MassARRAY®DNA质谱阵列基因分析系统毋需杂交，不存在潜在的杂交错配干扰，毋需荧光染料等标记物，直接以核酸片段的分子量为标记对核酸进行精确的定性定量分析，适用于各种类型的核酸分析实验。该技术一经问世很快就成为了国际业界公认的基因型分析和DNA甲基化分析的黄金标准。

 

 

 

 

Genotyping
Methylation Analysis
Molecular Typing
Somatic Mutation Profiling
Quantitative Gene Expression (QGE)

Genetic Analysis System*

Overview
Next-generation sequencing (NGS) and genome-wide association studies (GWAS) have revolutionized the genetic landscape, leading to the discovery of genetic markers with potential relevance for personalized medicine and pharmacogenomics. However, all genetic marker candidates – whether they are particular polymorphic loci, gene transcripts, or methylated regions – require further validation in order to advance to the next stage of utility and potentially lead to a molecular diagnostic test. An ideal method should be flexible and scalable in throughput to evaluate a varying number and type of markers. The MassARRAY system, available in 96- and 384-SpectroCHIP? formats, affords this capability.

 

MassARRAY System – an ideal genetic analysis system
The MassARRAY system offers MALDI-TOF mass spectrometry (MS) for high accuracy and sensitivity,robust chemistry for reproducible results, and advanced data analysis software to meet the needs of any genomic laboratory. The MassARRAY system, a scalable platform with a suite of applications for quantitative and qualitative nucleic acid analysis, provides flexible assay design, fast time-to-results, and cost effective sample run from tens to thousands of samples daily.

 

These features make it the ideal genetic analysis system for validation and fine mapping studies in basic and translational research settings.

The Complete Solution for Genomic Analysis
^ High Performance – Accurate MALDI-TOF MS detection provides unparalleled specificity and sensitivity for the most reliable results
^ Maximum Flexibility – Analyze any combination of SNPs and samples to meet varying study requirements
^ Highly Scalable – 96- and 384-well options for high and low-throughput applications
^ Easy-to-Use – New data acquisition software streamlines your workflow, and robust assay design software automates primer design and optimization to maximize efficiency and minimize experimental variability
^ A Variety of Genomic Applications – Software packages covering somatic mutations analysis,genotyping, methylation analysis and quantitative applications (gene expression and CNV) for easy and integrated workflow and short turnaround time

 

 

Applications
The MassARRAY system is a flexible platform that allows researchers to perform a variety of DNA analysis applications. The following applications are currently available:

Somatic Mutation Profiling
Recent research studies suggest genetic variants play a significant role in tumorigenesis, pathogenesis, and disease progression1,2. Oncogene mutations are key to cancer research. A group at Dana Farber Cancer Institute developed a high-throughput method for genotyping based on the MassARRAY system with over 200 mutations and discovered that >30% of 1,000 tumor samples carried at least one mutation3.The group has since developed other panels4.Sequenom offers a family of pre-designed assays,OncoCarta? Panels (v1.0, v2.0, v3.0), for comprehensive mutation screening of solid tumors, as well as a tissue specific melanoma panel, MelaCarta? Panel v1.0. Other options include targeted and custom panels through Assays by Sequenom. Visitwww.sequenom.com/exploreto see a menu of assays for somatic mutation profiling,or request ones specific to your research interests.

Genotyping
iPLEX Gold is the leading technology for SNP genotyping. The MassARRAY system is widely used for fine mapping and validation of GWAS studies, as well as genetic testing of SNP panels of interest. MassARRAY combines the benefits of a simple and accurate primer extension chemistry with state of the art MALDI-TOF mass spectrometry to quickly and cost effectively characterize genotypes with the highest levels of accuracy and reproducibility.The iPLEX Gold assay has been used in hundreds of publications to design assays at a multiplexing level of up to 36-plex, offering a high level of flexibility and a low cost per genotype.

Methylation Analysis
MassARRAY EpiTYPER? for quantitative DNA methylation analysis combines base-specific enzymatic cleavage with MALDI-TOF mass spectrometry.This combination creates a highly accurate, sensitive, and high-throughput method for the quantitative analysis of DNA methylation. MassARRAY EpiTYPER is scalable and allows you to analyze multiple CpGs on a single amplicon without compromising accuracy, sensitivity,or reproducibility. The EpiTYPER software provides convenient solutions for data analysis and export.

 

Molecular Typing
The MassARRAY system with iSEQ? software sets a new precedent for rapid, accurate molecular identification of microbes, viruses, and other haploid organisms. By combining the sensitivity of PCR and the accuracy of MALDI-TOF Mass Spectrometry, you can analyze one or more target regions on multiple samples in a convenient, homogeneous assay format.

Quantitative Gene Expression (QGE)
QGE combines competitive PCR with MALDI-TOF mass spectrometry enabling a highly accurate, sensitive, and high-throughput method for the quantitative analysis of gene expression. MassARRAY QGE is scalable, allowing you to perform assays without compromising accuracy,sensitivity, or reproducibility. In addition, the method does not use chemiluminescence, fluorescence or other secondary labeling approaches, making the approach cost-effective, in addition to enabling higher levels of multiplexing (up to 24-plex) end unparalleled precision.MassARRAY QGE is the method of choice for validation of microarray data, for the investigation of coding and noncoding transcripts – and whenever sensitivity and accuracy are required in transcription analysis.

 

 

Next Generation Genetic Analysis System

MassARRAY? Analyzer 4
With its exquisite sensitivity and accuracy, mass spectrometry is ideally suited for the analysis of nucleic acids. The new MassARRAY? Analyzer 4 system brings the power of mass spectrometry, robust molecular biology, and advanced data analysis software to meet the needs of high and low-throughput.

It provides increased throughput, faster time-to-results, and the ability to cost-effectively run from tens to thousands of samples – making this an ideal genetic analysis system for use in basic and translational research laboratories.

Setting new standards in speed, performance, and flexibility
With the new MassARRAY Analyzer 4 system you can:
Conduct rapid, accurate, and cost-effective analyses with a highly sensitive and specific mass spectrometry-based detection system
Run a variety of applications, from genotyping and somatic mutation analysis to methylation and comparative sequencing
Save research time and maximize efficiency with easy-to-use multiplexed assay design and optimization software
Fit experiments into your schedule with fast experimental setup and rapid data acquisition and analysis
Save money with simple, robust, label-free primer extension  chemistries for detection on the MassARRAY Analyzer 4 system

High Performance
Highly accurate MALDI-TOF-based detection provides unparalleled specificity and sensitivity for studies of genetic variation including somatic mutation detection in heterogeneous samples. The increased chip scan speed coupled with the robust  detection system gets you to results faster with minimal system down time.

Easy-to-Use
Robust assay design software automates primer design and optimization to maximize efficiency and minimize experimental variability. New data acquisition software streamlines your workflow to ensure fast, direct identification of genetic targets

Maximum Flexbility
The MassARRAY Analyzer 4 system has small footprint, and is a highly scalable platform with the flexibility to analyze any combination of SNPs and samples for low to high-throughput applications, making it ideally suited for fine mapping studies,and candidate gene analysis downstream of whole genome association studies.

Versatility
The benchtop MassARRAY Analyzer 4 system is ideally suited for targeted genetic analyses across a variety of applications including SNP genotyping, somatic mutation detection,methylation analysis, quantitative gene expression analysis,copy number variation, and comparative sequence analysis.

 
 

The MassARRAY Analyzer 4 system is offered in several configurations to meet your throughput needs. A 384-well system is intended for higher-throughput laboratories with a capacity of up to 138,000 genotypes per day. The 96-well system is specifically designed to address the needs of low to mid-throughput labs, and is ideally suited for experiments involving fewer than 30,000 genotypes per day (see table). For ordering information please contact your local office below.

 

MassARRAY^®DNA质谱阵列基因分析系统组成：

1、MassARRAY^®主机（第四代）

2、MassARRAY^®微量点样系统

3、MassARRAY^®DNA质谱阵列基因系统专用自动液体处理仪（选配）

4、强大的数据处理及分析软件包（选配）

²      Typer™SNP基因型软件

²      QGE™基因表达及CNV定量软件

²      EpiTYPER™DNA甲基化定量软件

²      iSEQ™病原体分子分型软件

 

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