特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
JN0048型2×BloTaq PCR MasterMix(血液直接PCR)北京厂家的品Pai:百奥莱博,是优质的核酸扩增(PCR)产品,用于科研目的生化试验项目,本产品质量好,且极具价格优势,深为用户称道,了解更多2×BloTaq PCR MasterMix(血液直接PCR)等核酸扩增(PCR)产品请联*(代"系")我司咨询订购。
名称:JN0048型2×BloTaq PCR MasterMix(血液直接PCR)北京厂家
编号:JN0048
产地:国产|进口
规格:1ml
品Pai:百奥莱博
Blo Taq是截短后的Taq DNA聚合酶,它缺失了5"-3"外切酶活性。Blo Taq还有其它突变,这些突变使它能够耐受全血中存在的YZ剂。此酶能够扩增人和小鼠全血样品中的DNA而无需事先提纯。Blo Taq可耐受高达体系中10%的全血。在大多数抗凝剂存在下Blo Taq依然表现良好,包括肝素、柠檬酸和EDTA。本制品是将PCR反应所需的Blo Taq DNA聚合酶、dNTP混合物、MgCl2以及反应缓冲液预先配制成2倍浓度的混合物,专为常规PCR扩增反应优化。使用时只需再加入模板和引物并稀释到1倍浓度即可进行PCR反应,大大地简化了操作过程,减少了PCR操作过程的污染。2X Blo Taq PCR MasterMix提供普通型/快速上样型两种形式供您选择。经测试,染料的加入不影响PCR反应,在PCR反应完成后可直接电泳,节省时间。
产品特点:
简便:直接用血液为模板扩增目的基因,无需事先提纯(图例一)。
GX:有效减少了杂带产生,从而实现高特异性的PCR。
稳定:经测试,反复冻融40 次后,扩增性能仍不受影响。
产品用途:
1、直接用血液为模板进行PCR 扩增目的基因,可进行血液相关基因疾病的快速诊断;
2、基因组扩增检测。
使用建议:建议使用时添加≤10%的全血为模板,加上血液后建议用涡旋器将管中各组分轻轻混匀。
应用实例:
图例一)以人全血(EDTA 抗凝)为模板,加入不同血液量扩增
泳道1:10%全血
泳道2:5%全血
泳道3:2%全血
泳道4:0%全血
泳道M:DL2000 Marker
常用反应体系(50μl)
成分 | 体积 |
2×BloTaq PCR MasterMix | 12.5μl |
上游引物 | 0.2-1.0μM(终浓度) |
下游引物 | 0.2-1.0μM(终浓度) |
全血 | 0.1-2.5ul |
ddH2O | 至25μl |
*Mg2+终浓度为3.75mM |
常用PCR循环:
当扩增片段<3K: |
94℃ | 1分钟30秒 |
30次循环 | 94℃,20秒 55℃,20秒 72℃,60秒/kb |
72℃ | 5分钟 |
4℃ | 保温 |
想要了解更多关于
JN0048型2×BloTaq PCR MasterMix(血液直接PCR)北京厂家的价格、品Pai、厂家、说明书、促销信息,请和我们联*(代"系")。此外我公司还销*(代"售")下面的产品:
·重组人泛素结合酶E2-C(UBCE2C)
编号:JN1007
英文名称:Recombinant Human Ubiquitin-Conjugating Enzyme E2 C
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Pro179在N端含有His标签。
UBE2C质量控制:>95%(还原性SDS-PAGE)
UBE2C制剂:液体
UBE2C保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于UBE2C:
Ubiquitin-Conjugating Enzyme E2 C (UBE2C) is a 179 amino acid enzyme that belongs to the Ubiquitin-Conjugating Enzyme family.UBE2C is highly expressed in tumor tissues and at low levels in most adult normal tissues. UBE2C is required for the destruction of mitotic cyclins and for cell cycle progression. UBE2C accepts Ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), which has E3 ubiquitin ligase activity, and targets for destruction substrates from the preceding mitosis (Cyclin A, Cyclin B, Securin, Geminin).
JN0048型2×BloTaq PCR MasterMix(血液直接PCR)北京厂家关键词:2×BloTaq PCR MasterMix(血液直接PCR),百奥莱博,JN0048
·重组人雌激素受体α
编号:JN0857
英文名称:Recombinant Human Estrogen receptor alpha
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Gln116在N端含有His标签。
Estrogen Receptor质量控制:>95%(还原性SDS-PAGE)
Estrogen Receptor制剂:冻干品
Estrogen Receptor保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
Estrogen Receptor复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于Estrogen Receptor:
Estrogen Receptor is a major ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily. Estrogen Receptor is composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but they also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5 UTRs and use different promoters.
JN0048型2×BloTaq PCR MasterMix(血液直接PCR)北京厂家关键词:2×BloTaq PCR MasterMix(血液直接PCR),百奥莱博,JN0048
·重组人B3GAT3
编号:JN1385
英文名称:Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Glu72-Val335在C端含有His标签。
B3GAT3质量控制:>95%(还原性SDS-PAGE)
B3GAT3制剂:液体
B3GAT3保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于B3GAT3:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family. B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins , hows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.
我公司正在优惠促销核酸扩增(PCR)等系列产品,期待您的咨询选购JN0048型2×BloTaq PCR MasterMix(血液直接PCR)北京厂家。
温馨提示:不可用于临床ZL。