产地:Qiagen货号:12843ApplicationsCompactPrep Plasmid Kits provide molecular biology grade plasmid DNA with low endotoxin levels (see figure "Low Endotoxin Levels") suitable for use in routine applications, including: High-quality automated sequencing Restriction digestion Ligation and transformation In vitro transcription/translation Transfection of robust cells Principle and procedureCompactPrep Plasmid Kits provide fast, large-scale, non-chaotrophic silica-based plasmid DNA preparation. DNA binding and washing using a microspin column on a vacuum manifold (e.g., QIAvac 24 Plus) and DNA elution using a microcentrifuge enables the whole plasmid purification process to take place at the bench. Maximum plasmid yields using LyseBlue LyseBlue reagent is now also included in CompactPrep Kits so you can be confident of obtaining the best possible DNA yields. Using a simple visual identification system, LyseBlue reagent prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, cell debris, and genomic DNA (see figure "Visualisation of Efficient Cell Lysis and SDS Precipitation Using LyseBlue"). LyseBlue is optional and provided in a separate vial within the kit.