产地:Vectorlabs 货号:H-1200规格: 10 mlThis special formulation of VECTASHIELD® Mounting Medium contains a counterstain for DNA and is ready to use for fluorescent in situ hybridization (FISH) or any other procedures requiring fluorescent counterstaining of DNA. Counterstain may also stain RNA. Counterstain is provided at an optimal concentration of 1.5 µg/ml. DAPI (4’,6 diamidino-2-phenylindole) produces a blue fluorescence with excitation at about 360 nm and emission at about 460 nm when bound to DNA. There is no emission overlap with fluorescein, rhodamine or Texas Red®. Unlike mountants which harden, such as VECTASHIELD® HardSet™, VECTASHIELD® Mounting Medium is an aqueous mountant which does not solidify but remains a viscous liquid on the slide. To mount tissues or cells on a slide, a special pipet is supplied with the product to dispense one drop (approximately 25µl) onto the section. After a cover slip is applied, the VECTASHIELD® Mounting Medium is allowed to disperse over the entire section. (25µl is sufficient for a 22 x 22 mm coverslip). After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 ºC. The fluorescence will remain for many weeks.