27361 DirectPrep 96 MiniPrep Kit;Qiagen 27361;原装

产地：Qiagen货号：27361ApplicationsThe DirectPrep 96 Miniprep Kit provides ready-to-use plasmid DNA of a yield and quality suitable for all standard downstream applications and is especially suited for high-throughput applications, including: Automated fluorescent sequencing (see figure "High-Quality DNA for Automated Sequencing") Restriction digestion PCRProcedureAll lysis steps are carried out in the same 96-well flat-bottom block used for growth of the bacteria. After alkaline lysis of the cells, the lysate is neutralized using an optimized buffer, which leads to a virtually clear lysate. Centrifugation or filtration of the lysate is not required. Isopropanol is added in order to optimize the DNA binding conditions, and lysates are transferred to the activated DirectPrep 96 plate where all subsequent steps take place (see flowchart "DirectPrep 96 Procedure"). Plasmid DNA binds to the membrane, which is subsequently washed. After washing, the membrane is dried and the bound plasmid DNA is eluted. The DirectPrep 96 Miniprep procedure gives typical yields of up to 4 µg DNA from 1.25 ml E. coli culture grown in LB medium. PrincipleThe DirectPrep 96 Miniprep method of high-throughput plasmid DNA isolation uses a novel lysis chemistry that eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using only one plate and a simple bind-wash-elute procedure. This results in a protocol that is easier to perform than most existing methods. The DirectPrep 96 procedure is designed for use on the QIAvac Multiwell.