特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
北京百奥莱博专业生产供应BL21(DE3)感受态细胞(pTf16分子伴侣) 克隆与表达,我公司销*(代"售")全品类的分子生物学试剂,价格优惠,欢迎广大科研工作者垂询订购。
名称:BL21(DE3)感受态细胞(pTf16分子伴侣) 克隆与表达
品Pai:百奥莱博
规格:100μl×10
英文名:Chaperone competent cells pTf16/BL21(DE3)
外源蛋白质在大肠杆菌中的表达常形成包涵体或发生蛋白酶降解。大多是由于所表达的蛋白未能正确折叠造成。分子伴侣协同作用,可以帮助外源蛋白正确折叠。不同外源蛋白需要的分子伴侣不同。百奥莱博提供5种表达常用分子伴侣的BL21(DE3)衍生菌株,适用于不同外源蛋白在大肠杆菌中有效折叠。该系统适用于pET系列等依赖T7启动子的表达载体。本感受态细胞无污染,转化效率可达107以上
![](http://item.yiqi.com/pic/ConPic/4/JN0128.jpg)
图例一)Human种属的RORG(31KD)在大肠杆菌中获得可溶表达。
Lane 1:上样
Lane 2: 流穿
Lane 3: 50mM Imidazole洗脱
Lane 4: 500mM Imidazole洗脱
Lane 5: 蛋白Marker
![](http://item.yiqi.com/pic/ConPic/4/%E7%94%9F%E5%8C%96%E8%AF%95%E5%89%82%20(252).jpg)
欲咨询购买BL21(DE3)感受态细胞(pTf16分子伴侣) 克隆与表达,请致电北京百奥莱博科技有限公司,为您提供最全面周到的产品服务,除此之外,我公司正在促销以下产品:
·重组人LAMP2
编号:JN1840
英文名称:Recombinant Human Lysosome-Associated Membrane Glycoprotein 2
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Leu29-Ile375在C端含有His标签。
LAMP2质量控制:>95%(还原性SDS-PAGE)
LAMP2制剂:冻干品
LAMP2保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
LAMP2复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于LAMP2:
Lysosomal Associated Membrane Protein 2 (LAMP2) is a major component of lysosomal membranes. LAMP2 is a transmembrane glycoprotein about 110kDa. Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail . The lumenal domain is organized into two heavily N-glycosylated regions. Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, cytoplasmic tail and transmenmbrane segment.LAMP2 itself can cleavage lysosomal luminal domain and degradation lysosomal. In the help of chaperone HSC73,LAMP2 mediates the lysosomal uptake in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles.
BL21(DE3)感受态细胞(pTf16分子伴侣) 克隆与表达关键词:JN0128,Chaperone competent cells pTf16/BL21(DE3),pTf16分子伴侣,BL21(DE3)感受态细胞(pTf16分子伴侣)
![](http://item.yiqi.com/pic/ConPic/4/%E7%94%9F%E5%8C%96%E8%AF%95%E5%89%82%20(236).jpg)
·重组人C型凝集素结构域家族3成员B(CLEC3B)
编号:JN0572
英文名称:Recombinant Human C-Type Lectin Domain Family 3 Member B
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Glu22-Val202在C端含有His标签。
CLEC3B质量控制:>95%(还原性SDS-PAGE)
CLEC3B制剂:冻干品
CLEC3B保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
CLEC3B复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于CLEC3B:
C-Type Lectin Domain Family 3 Member B (CLEC3B) is a serum and tissue protein and it contais a C-type lectin which binds to Ca++. CLEC3B is originally found in plasma, the concentrations approximately 10mg/l. It can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine. In addition, CLEC3B may be involved in the packaging of molecules destined for exocytosis. Also, CLEC3B is best known as a prognostic marker in ovarian cancer.
BL21(DE3)感受态细胞(pTf16分子伴侣) 克隆与表达关键词:JN0128,Chaperone competent cells pTf16/BL21(DE3),pTf16分子伴侣,BL21(DE3)感受态细胞(pTf16分子伴侣)
![](http://item.yiqi.com/pic/ConPic/4/%E7%94%9F%E5%8C%96%E8%AF%95%E5%89%82%20(170).jpg)
·重组人PPP1CC(丝*酸/苏*酸蛋白*(代"磷")酸酶1C催化亚基)
编号:JN1752
英文名称:Recombinant Human Serine/Threonine-Protein Phosphatase PP1-γ Catalytic Subunit
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Lys323在N端、C端都带有His标签。
PPP1CC质量控制:>95%(还原性SDS-PAGE)
PPP1CC制剂:液体
PPP1CC保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于PPP1CC:
Serine/Threonine-Protein Phosphatase PP1-Υ Catalytic Subunit (PPP1CC) is a member of the PPP phosphatase family. It is essential for cell division, participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CC colocalizes with SPZ1 in the nucleus, with URI1 at mitochondrion, Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic comp*(代"a")rtments. As a cofactor, PPP1CC binds one iron ion and one manganese ion per subunit.. In addition, PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
![](http://item.yiqi.com/pic/ConPic/4/%E7%94%9F%E5%8C%96%E8%AF%95%E5%89%82%20(314).jpg)
我公司生产供应销*(代"售")的克隆与表达产品正全系列现货促销,满分期待您的垂询选购BL21(DE3)感受态细胞(pTf16分子伴侣) 克隆与表达。
温馨提示:不可用于临床ZL。