Agilent安捷伦 SureSelect SS RNA Library 链特异性RNA转录组建库试剂盒 G9691B

Whole-Transcriptome

Agilent's SureSelect Strand Specific RNA Library Preparation Kit is the highest sensitivity, strand-specific method for preparing libraries for mRNA or targeted RNA-seq, enabling a greater understanding of gene regulation.

SureSelect Strand Specific RNA Library Preparation Kit:

• High strand specificity to discern overlapping transcripts and investigate antisense transcription（具有高度链特异性，可深入了解基因调控）
• Greater library complexity using small amounts of starting material, as low as 50 ng of total RNA（使用少量起始材料就能构建更复杂的文库）
• Best uniformity of coverage with less 5’/3’ bias（覆盖度更均一且5'/3'偏向性更小）

Strand Specific RNA Library Prep - Details & Specifications

（以更佳的性能和特异性获得更多信息）

 

Outstanding Performance and Strand Specificity for mRNA and Targeted RNA Sequencing

Discover More with Greater Performance and Specificity Agilent’s SureSelect Strand Specific RNA Library Preparation Kit offers a highly sensitive, strand-specific method for preparing libraries for mRNA or targeted RNA sequencing. The RNA library prep kit provides high strand specificity with greater uniformity of coverage across the entire transcript, enabling a greater understanding of gene regulation. It also provides unmatched accuracy for better discovery of novel transcripts and gene fusions. The kit has been designed to maintain high library complexity even with small amounts of starting material, as low as 50ng of total RNA, for studies with limited samples amounts.（安捷伦的SureSelect链特异性RNA文库制备试剂盒采用高度灵敏的链特异性方法制备全转录组或靶向RNA测序的文库。RNA文库制备试剂盒提供了高度链特异性，对于完整转录本具有更高的覆盖均一性，从而能够深入了解基因调控。其还未更好的揭示新转录本和融合基因提供了无与伦比的精确度。该试剂盒经特殊设计，能够维持高文库复杂度，即使对于样品量有限的研究，使用低至50ng总RNA的少量起始材料也是如此）。

（图1, 与其它竞争对手的试剂盒对比，SureSelect链特异性RNA文库制备试剂盒对于完整转录本显示出了更佳的覆盖均一性）

Complete Kits for RNA-Seq and Gene Regulation Studies（用于RNA测序和基因调控研究的全面试剂盒）

Next Generation Sequencing for studying gene regulation is becoming widely adopted and strand specific information can greatly enable further understanding. The SureSelect Strand Specific RNA Library Prep Kit has also been designed to be as complete as possible, including master-mixed reagents and streamlined workflow for less hands-on-time and faster time to results. The SureSelect RNA Library Preparation Kit is an integral part of Agilent’s complete NGS Gene Regulation solutions, including SureSelect RNA Target Enrichment, SureSelect Human Methyl-Seq, and GeneSpring NGS data analysis.（用于基因研究调控的新一代测序正广为接纳，并且链特异性信息可极大的加深对基因调控的理解。SureSelect链特异性RNA文库制备试剂盒还经过特别设计，以做到尽可能的全面，包括有预混合试剂和简化的工作流程，以缩短操作时间并更快的获得结果。SureSelect RNA测序文库制备试剂盒是安捷伦的全面NGS基因调控解决文案组成部分，该方案包括SureSelect RNA靶向序列捕获，SureSelect人甲基化测序和GeneSpring NGS数据分析）。

（图2 SureSelect RNA 测序文库制备试剂盒可用于全转录组或靶向RNA测序分析，图中对肿瘤和正常样本做了对比，显示了以人RNA 激酶组试剂盒获得的靶向序列捕获结果）

（图3 使用相同样品和仅仅50ng的起始RNA进行RNA测序，结果显示出与微阵列数据的高度相关性。因此使用这两种互补性方法更容易进行跟踪和验证）