特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
北京百奥莱博主要从事生物试剂的生产和销*(代"售"),产品线涵盖重组人半乳糖激酶(Galactokinase)怎么卖在内的分子生物学试剂产品,还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。
名称:重组人半乳糖激酶(Galactokinase)怎么卖
产地:国产|进口
规格:10μg|50μg|500μg|1mg
编号:JN0888
英文名:Recombinant Human Galactokinase
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Leu392在C端含有His标签。
Galactokinase质量控制:>95%(还原性SDS-PAGE)
Galactokinase制剂:液体
Galactokinase保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于Galactokinase:
Galactokinase belongs to the GHMP kinase family and GalK subfamily. Galactokinase is more important to the galactose metabolism pathway. It modifies galactose to create a similar molecule called Galactose-1-Phosphate.Thus, a series of additional steps converts Galactose-1-Phosphate to another simple sugar Glucose, which is the main energy source for most cells. Galactokinase deficiency results in Galactosemia II, an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of Galactitol in the lenses.
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·重组人BDNF(脑源性神经生长因子)
编号:JN0220
英文名称:Recombinant Human Brain-Derived Neurotrophic Factor
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备,目的基因编码的His129-Arg247表达纯化而来。
BDNF质量控制:>95%(还原性SDS-PAGE)。
BDNF制剂:冻干品
BDNF保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
BDNF复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于BDNF:
Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson"s and Alzheimer"s disease.
重组人半乳糖激酶(Galactokinase)怎么卖关键词:重组半乳糖激酶,JN0888,重组人Galactokinase,重组人半乳糖激酶,重组人半乳糖激酶蛋白
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·BL21(DE3)感受态细胞(pKJE7分子伴侣)
编号:JN0126
英文名称:Chaperone competent cells pKJE7/BL21(DE3)
规格:100μl×10
外源蛋白质在大肠杆菌中的表达常形成包涵体或发生蛋白酶降解。大多是由于所表达的蛋白未能正确折叠造成。分子伴侣协同作用,可以帮助外源蛋白正确折叠。不同外源蛋白需要的分子伴侣不同。百奥莱博提供5种表达常用分子伴侣的BL21(DE3)衍生菌株,适用于不同外源蛋白在大肠杆菌中有效折叠。该系统适用于pET系列等依赖T7启动子的表达载体。本感受态细胞无污染,转化效率可达107以上

图例一)Human种属的RORG(31KD)在大肠杆菌中获得可溶表达。
Lane 1:上样
Lane 2: 流穿
Lane 3: 50mM Imidazole洗脱
Lane 4: 500mM Imidazole洗脱
Lane 5: 蛋白Marker
·重组人EphA2
编号:JN1572
英文名称:Recombinant Human Ephrin A Receptor 2
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Ala24-Asn534在C端带有Fc标签。
EphA2质量控制:>95%(还原性SDS-PAGE)
EphA2制剂:冻干品
EphA2保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
EphA2复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于EphA2:
Ephrin type-A receptor 2/EphA2 is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5. A and B class Eph proteins have a common structural organization. Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neioring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. EphA2 becomes autophosphorylated following ligand binding and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways. Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin, and can be induced by p53 family transcription factors. EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells. In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels. EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration.
重组人半乳糖激酶(Galactokinase)怎么卖关键词:重组半乳糖激酶,JN0888,重组人Galactokinase,重组人半乳糖激酶,重组人半乳糖激酶蛋白
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