Biotinylated Mouse BAFFR /TNFRSF13C Protein, Fc Tag, Avi Tag (Avitag™)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA. Immobilized Human BAFF, Fc Tag (Catalog # BAF-H4268) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse BAFFR, Fc Tag (Catalog # BAR-M82F0) with a linear range of 0.5-8 ng/mL.

产品背景:
BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

产品描述:
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 35.1 kDa. As a result of glycosylation, the protein migrates as 45-60 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).
 SDS-PAGE
Biotinylated Mouse BAFFR /TNFRSF13C, Fc Tag, Avi Tag (Avitag™) on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.