pGreenII0179载体

LOCUS       Exported File           5138 bp ds-DNA    circular SYN 21-四月-2013DEFINITION  Agrobacterium binary vector with kanamycin- and             hygromycin-resistance genes.ACCESSION   .VERSION     .KEYWORDS    pGreenII 0179SOURCE      http://www.biofeng.com/  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 5138)  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.  TITLE     pGreen: a versatile and flexible binary Ti vector for             Agrobacterium-mediated plant transformation.  JOURNAL   Plant Mol. Biol. 2000;42:819-32.  PUBMED    10890530REFERENCE   2  (bases 1 to 5138)  AUTHORS   pGreen website  TITLE     Direct Submission  JOURNAL   Exported 2016-07-14 from SnapGene Viewer 1.5.3            http://www.snapgene.comCOMMENT     This plasmid must be transformed into an Agrobacterium strain that             also carries a vector such as pSoup.FEATURES             Location/Qualifiers     source          1..5138                     /organism="synthetic DNA construct"                     /lab_host="Plant Cells"                     /mol_type="other DNA"     rep_origin      63..498                     /gene="                     "                     /note="pSa ori"                     /note="origin of replication from bacterial plasmid pSa"                     /note="color: #ffff00"     misc_feature    633..655                     /note="LB T-DNA repeat"                     /note="left border repeat from nopaline C58 T-DNA                      (truncated)"                     /note="color: #ffe4c4"     polyA_signal    663..839                     /note="CaMV poly(A) signal"                     /note="cauliflower mosaic virus polyadenylation signal"                     /note="color: #a6acb3"     CDS             complement(1049..2074)                     /codon_start=1                     /product="hygromycin B phosphotransferase"                     /note="HygR"                     /note="confers resistance to hygromycin"                     /note="color: #ccffcc"                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK                     E"     promoter        complement(2091..2436)                     /note="CaMV 35S promoter"                     /note="strong constitutive promoter from cauliflower mosaic                     virus"                     /note="color: #ffffff; direction: LEFT"     CDS             complement(2434..2871)                     /codon_start=1                     /gene="lacZ (truncated)"                     /product="LacZ-alpha fragment of?beta-galactosidase"                     /note="lacZ-alpha"                     /note="color: #993366"                     /translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI                     PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART                     DRPSQQLRSLNGEWREIVNVNVIVPLLQKCQRYSLRRPKGY"     primer_bind     2658..2674                     /note="M13 fwd"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: RIGHT"     promoter        2681..2699                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"                     /note="color: #ffffff; direction: RIGHT"     misc_feature    2708..2815                     /note="MCS"                     /note="pBluescript multiple cloning site"                     /note="color: #99ccff"     promoter        complement(2828..2846)                     /note="T3 promoter"                     /note="promoter for bacteriophage T3 RNA polymerase"                     /note="color: #ffffff; direction: LEFT"     primer_bind     complement(2867..2883)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: LEFT"     protein_bind    2891..2907                     /bound_moiety="lac repressor encoded by lacI"                     /note="lac operator"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."                     /note="color: #31849b"     promoter        complement(2915..2946)                     /note="lac promoter"                     /note="promoter for the E. coli lac operon"                     /note="This reverse directional feature has 3 segments:                     ???1:?2915?..?2921?/?#ffffff?/?-10                     ???2:?2922?..?2940?/?#ffffff                     ???3:?2941?..?2946?/?#ffffff?/?-35"     misc_feature    3221..3245                     /note="RB T-DNA repeat"                     /note="right border repeat from nopaline C58 T-DNA"                     /note="color: #ffe4c4"     rep_origin      complement(3336..3924)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"                     /note="color: #ffff00"     CDS             complement(4095..4910)                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /note="color: #ccffcc"                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"ORIGIN        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta      661 tcgatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag      721 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac      781 cctataagaa ccctaatttc ccttatcggg aaactactca cacattattt atggagaaaa      841 tagagagaga tagatttgta gagagagact ggtgatttca gcgtaccgaa ttaattctcc      901 cgcgaccagc cgagcgagct tagcgaactg tggacgagaa ctgtgccacc aagcgtaagg      961 ccgttctctc gcatttgcct tgctaggctc gcgcgagttg ctggctgagg cgttctcgaa     1021 atcagctctt gttcggtcgg catctactct attcctttgc cctcggacga gtgctggggc     1081 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc     1141 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc     1201 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt     1261 caagaccaat gcggagcata tacgcccgga ggcgtggcga tcctgcaagc tccggatgcc     1321 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga     1381 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg     1441 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc     1501 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg     1561 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc     1621 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc     1681 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggctgaa     1741 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg     1801 agatgcaata ggtcaggctc tcgctgaact ccccaatgtc aagcacttcc ggaatcggga     1861 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc     1921 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt     1981 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct     2041 cgacagacgt cgcggtgagt tcaggctttt tcatagggat cagcttgggc tgtcctctcc     2101 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg     2161 tcatccctta cgtcagtgga gatgtcacat caatccactt gctttgaaga cgtggttgga     2221 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg     2281 gcagaggcat cttgaatgat agcctttcct ttatcgcaat gatggcattt gtaggagcca     2341 ccttcctttt ctactgtcct ttcgatgaag tgacagatag ctgggcaatg gaatccgagg     2401 aggtttcccg aaattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact     2461 gtatctttga catttttgga gtaggggtac gataacatta acgtttacaa tttcgcgcca     2521 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     2581 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     2641 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg     2701 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt     2761 cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct     2821 tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc     2881 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga aghcataaag     2941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg     3001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg     3061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc     3121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc     3181 acagaatcag gggataacgc aggaaagaac atgaaggcct tgacaggata tattggcggg     3241 taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag     3301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     3361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     3421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     3481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     3541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     3601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     3661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     3721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     3781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaagaagagt tggtagctct     3841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     3901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     3961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     4021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa     4081 cattggtcta gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata     4141 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca     4201 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca     4261 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca     4321 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact     4381 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta     4441 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta     4501 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca     4561 cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg     4621 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat     4681 tccgtcagcc agtttagtct gaccatctca tctgtaacaa cattggcaac gctacctttg     4741 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca     4801 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg     4861 gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacacccctt     4921 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt     4981 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt     5041 gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag     5101 caaaagttca aaatcaccaa ctggtccacc tacaacaa