供应商: 泽叶生物 数量: 20 英文名: pGreenII0049 保质期: 三年 保存条件: -20℃低温保存
出品公司: | ZYbscience |
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载体名称: | pGreenII0049 ; pGreenII 0049 |
质粒类型: | 植物载体;农杆菌双元表达载体;荧光素酶报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | lacZ |
载体大小: | 7065 bp |
5' 测序引物及序列: | M13 Forward:TGTAAAACGACGGCCAGT |
3' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
载体标签: | 荧光素酶 |
载体抗性: | 卡那霉素 |
筛选标记: | Neomycin |
克隆菌株: | HB101等菌株 |
宿主细胞(系): | 农杆菌/植物细胞 |
备注: | -- |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 非病毒 |
LOCUS Exported File 7065 bp ds-DNA circular SYN 14-七月-2016DEFINITION Agrobacterium binary vector with kanamycin-resistance and luciferase genes.ACCESSION .VERSION .KEYWORDS pGreenII 0049SOURCE http://www.biofeng.com/ ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7065) AUTHORS Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM. TITLE pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. JOURNAL Plant Mol. Biol. 2000;42:819-32. PUBMED 10890530REFERENCE 2 (bases 1 to 7065) AUTHORS pGreen website TITLE Direct Submission JOURNAL Exported 2016-07-14 from SnapGene Viewer 1.5.3 http://www.snapgene.comCOMMENT This plasmid must be transformed into an Agrobacterium strain that also carries a vector such as pSoup.FEATURES Location/Qualifiers source 1..7065 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" rep_origin 63..498 /gene=" " /note="pSa ori" /note="origin of replication from bacterial plasmid pSa" /note="color: #ffff00" misc_feature 633..655 /note="LB" /note="left border repeat from nopaline C58 T-DNA (truncated)" /note="color: #ffe4c4" terminator 679..930 /note="NOS terminator" /note="nopaline synthase terminator and poly(A) signal" /note="color: #ffffff" CDS complement(970..1764) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(1798..1977) /note="NOS promoter" /note="nopaline synthase promoter" /note="color: #000000; direction: LEFT" CDS complement(2048..2437) /codon_start=1 /gene="lacZ (truncated)" /product="LacZ-alpha fragment of?beta-galactosidase" /note="lacZ" /note="color: #993366" /translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRSLNGEWREIVNVNVIDT" primer_bind 2224..2240 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: RIGHT" promoter 2247..2265 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: RIGHT" misc_feature 2274..2381 /note="MCS" /note="pBluescript multiple cloning site" /note="color: #99ccff" promoter complement(2394..2412) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" /note="color: #ffffff; direction: LEFT" primer_bind complement(2433..2449) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: LEFT" protein_bind 2457..2473 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /note="color: #31849b" promoter complement(2481..2512) /note="lac promoter" /note="promoter for the E. coli lac operon" /note="This reverse directional feature has 3 segments: ???1:?2481?..?2487?/?#ffffff?/?-10 ???2:?2488?..?2506?/?#ffffff ???3:?2507?..?2512?/?#ffffff?/?-35" polyA_signal 2787..2963 /note="CaMV poly(A)" /note="cauliflower mosaic virus polyadenylation signal" /note="color: #a6acb3" CDS complement(3079..4731) /codon_start=1 /gene="luc" /product="firefly luciferase" /EC_number=" " /note="luciferase" /note="color: #ffcc00" /experiment=" " /protein_id=" " /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" promoter complement(4742..5087) /note="CaMV 35S promoter" /note="strong constitutive promoter from cauliflower mosaic virus" /note="color: #000000; direction: LEFT" misc_feature 5148..5172 /note="RB" /note="right border repeat from nopaline C58 T-DNA" /note="color: #ffe4c4" rep_origin complement(5263..5851) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(6022..6837) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"ORIGIN 1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat 61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag 121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg 181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg 241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa 301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac 361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc 421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg 481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc 541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct 601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta 661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct 721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa 781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac 841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa 901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt ccgaacccca 961 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg 1021 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc 1081 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca 1141 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc 1201 ctgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc 1261 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc 1321 catccgagta cgtcctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc 1381 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg 1441 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct 1501 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca 1561 cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt cggtcttgac 1621 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat 1681 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc 1741 gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat gagactctaa 1801 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc 1861 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc 1921 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt 1981 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc 2041 ttaattctca tgtatcgata acattaacgt ttacaatttc gcgccattcg ccattcaggc 2101 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 2161 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 2221 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtaccg 2281 ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg 2341 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag 2401 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 2461 tatccgctca caattccaca caacatacga gccggaaghc ataaagtgta aagcctgggg 2521 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 2581 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 2641 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 2761 taacgcagga aagaacatga aggatcgatc tggattttag tactggattt tggttttagg 2821 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt 2881 atatgctcaa cacatgagcg aaaccctata agaaccctaa tttcccttat cgggaaacta 2941 ctcacacatt atttatggag aaaatagaga gagatagatt tgtagagaga gactggtgat 3001 ttcagcgtac cgaattaatt ctctagctag agtattacaa tagctaagaa tttcgtcatc 3061 gctgaataca gttacatttt acaatttgga ctttccgccc ttcttggcct ttatgaggat 3121 ctctctgatt tttcttgcgt cgagttttcc ggtaagacct ttcggtactt cgtccacaaa 3181 cacaactcct ccgcgcaact ttttggcggt tgttacttga ctggcgacgt aatccacgat 3241 ctctttttcc gtcatcgtct ttccgtgctc caaaacaaca acggcggcgg gaagttcacc 3301 ggcgtcatcg tcgggaagac ctgccacgcc cgcgtcgaag atgttggggt gttgtaacaa 3361 tatcgactcc aattcagcgg gggccacctg gtatcctttg tatttaatta aagacttcaa 3421 gcggtcaact atgaagaagt gttcgtcttc gtcccagtaa gctatgtctc cagaatgtag 3481 ccatccatcc ttgtcaatca aggcgttggt cgcttccgga ttgtttacat aaccggacat 3541 aatcataggt cctctgacac ataattcgcc tctctgatta acgcccagcg ttttcccggt 3601 atccagatcc acaaccttcg cttcaaaaaa tggaacaact ttaccgaccg cgcccggttt 3661 atcatccccc tcgggtgtaa tcagaatagc tgatgtagtc tcagtgagcc catatccttg 3721 tcgtatccct ggaagatgga agcgttttgc aaccgcttcc ccgacttctt tcgaaagagg 3781 tgcgccccca gaagcaattt cgtgtaaatt agataaatcg tatttgtcaa tcagagtgct 3841 tttggcgaag aatgaaaata gggttggtac tagcaacgca ctttgaattt tgtaatcctg 3901 aagggatcgt aaaaacagct cttcttcaaa tctatacatt aagacgactc gaaatccaca 3961 tatcaaatat ccgagtgtag taaacattcc aaaaccgtga tggaatggaa caacacttaa 4021 aatcgcagta tccggaatga tttgattgcc aaaaatagga tctctggcat gcgagaatct 4081 gacgcaggca gttctatgcg gaagggccac acccttaggt aacccagtag atccagagga 4141 gttcattatc agtgcaattg ttttgtcacg atcaaaggac tctggtacaa aatcgtattc 4201 attaaaaccg ggaggtagat gagatgtgac gaacgtgtac atcgactgaa atccctggta 4261 atccgtttta gaatccatga taataatttt ctggattatt ggtaattttt tttgcacgtt 4321 caaaattttt tgcaacccct ttttggaaac aaacactacg gtaggctgcg aaatgttcat 4381 actgttgagc aattcacgtt cattataaat gtcgttcgcg ggcgcaactg caactccgat 4441 aaataacgcg cccaacaccg gcataaagaa ttgaagagag ttttcactgc atacgacgat 4501 tctgtgattt gtattcagcc catatcgttt catagcttct gccaaccgaa cggacatttc 4561 gaagtattcc gcgtacgtga tgttcacctc gatatgtgca tctgtaaaag caattgttcc 4621 aggaaccagg gcgtatctct tcatagcctt atgcagttgc tctccagcgg ttccatcctc 4681 tagcggatag aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tgagcttggg 4741 ctgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt 4801 gggattgtgc gtcatccctt acgtcagtgg agatgtcaca tcaatccact tgctttgaag 4861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg 4921 gaccactgtc ggcagaggca tcttgaatga tagcctttcc tttatcgcaa tgatggcatt 4981 tgtaggagcc accttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat 5041 ggaatccgag gaggtttccc gaaattaccc tttgttgaaa agtctcaata gccctttggt 5101 cttctgagac tgtatctttg acatttttgg agtaggggta cgatccttga caggatatat 5161 tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca 5221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 5401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 5461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 5521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 5581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 5641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
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