pGreenII0229载体

LOCUS       Exported File           4448 bp ds-DNA    circular SYN 14-七月-2016DEFINITION  Agrobacterium binary vector with kanamycin-and             bialophos/phosphinothricin-resistance genes.ACCESSION   .VERSION     .KEYWORDS    pGreenII 0229SOURCE      http://www.biofeng.com/  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 4448)  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.  TITLE     pGreen: a versatile and flexible binary Ti vector for             Agrobacterium-mediated plant transformation.  JOURNAL   Plant Mol. Biol. 2000;42:819-32.  PUBMED    10890530REFERENCE   2  (bases 1 to 4448)  AUTHORS   pGreen website  TITLE     Direct Submission  JOURNAL   Exported 2016-07-14 from SnapGene Viewer 1.5.3            http://www.snapgene.comCOMMENT     This plasmid must be transformed into an Agrobacterium strain that             also carries a vector such as pSoup.FEATURES             Location/Qualifiers     source          1..4448                     /organism="synthetic DNA construct"                     /lab_host="Plant Cells"                     /mol_type="other DNA"     rep_origin      63..498                     /gene="                     "                     /note="pSa ori"                     /note="origin of replication from bacterial plasmid pSa"                     /note="color: #ffff00"     misc_feature    633..655                     /note="LB"                     /note="LB T-DNA repeat ?left border repeat from nopaline                      C58 T-DNA (truncated)"                     /note="color: #ffe4c4"     terminator      679..930                     /note="NOS terminator"                     /note="nopaline synthase terminator and poly(A) signal"                     /note="color: #ffffff"     CDS             complement(950..1501)                     /codon_start=1                     /gene="Streptomyces hygroscopicus bar"                     /product="phosphinothricin acetyltransferase"                     /function="                     "                     /note="BlpR"                     /note="confers resistance to bialophos or phosphinothricin"                     /note="color: #ccffcc"                     /protein_id="                     "                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIQTSTVNFRTEPQEPQE                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF                     WQLDFSLPVPPRPVLPVTEI"     promoter        complement(1544..1721)                     /note="NOS promoter"                     /note="nopaline synthase promoter"                     /note="color: #ffffff; direction: LEFT"     CDS             complement(1792..2181)                     /codon_start=1                     /gene="lacZ (truncated)"                     /product="LacZ-alpha fragment of?beta-galactosidase"                     /note="lacZ"                     /note="color: #993366"                     /translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI                     PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART                     DRPSQQLRSLNGEWREIVNVNVIDT"     primer_bind     1968..1984                     /note="M13 fwd"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: RIGHT"     promoter        1991..2009                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"                     /note="color: #ffffff; direction: RIGHT"     misc_feature    2018..2125                     /note="MCS"                     /note="pBluescript multiple cloning site"                     /note="color: #00ff00"     promoter        complement(2138..2156)                     /note="T3 promoter"                     /note="promoter for bacteriophage T3 RNA polymerase"                     /note="color: #ffffff; direction: LEFT"     primer_bind     complement(2177..2193)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: LEFT"     protein_bind    2201..2217                     /bound_moiety="lac repressor encoded by lacI"                     /note="lac operator"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."                     /note="color: #31849b"     promoter        complement(2225..2256)                     /note="lac promoter"                     /note="promoter for the E. coli lac operon"                     /note="This reverse directional feature has 3 segments:                     ???1:?2225?..?2231?/?#ffffff?/?-10                     ???2:?2232?..?2250?/?#ffffff                     ???3:?2251?..?2256?/?#ffffff?/?-35"     misc_feature    2531..2555                     /note="RB"                     /note="RB T-DNA repeat ?right border repeat from nopaline                      C58 T-DNA"                     /note="color: #ffe4c4"     rep_origin      complement(2646..3234)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"                     /note="color: #ffff00"     CDS             complement(3405..4220)                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /note="color: #ccffcc"                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"ORIGIN        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta      661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa      781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac      841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa      901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg      961 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca     1021 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccacgg ggggcatatc     1081 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc     1141 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc     1201 ccgtccgctg gtggcggggg gagacgtaca cggttgactc ggccgtccag tcgtaggcgt     1261 tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga     1321 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct     1381 gcggctcggt acggaagttg accgtgcttg tctggatgta gtggttgacg atggtgcaga     1441 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca     1501 tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg     1561 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac     1621 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac     1681 tccagaaacc cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa     1741 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctcatgtatc     1801 gataacatta acgtttacaa tttcgcgcca ttcgccattc aggctgcgca actgttggga     1861 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     1921 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     1981 cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     2041 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag     2101 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattccga     2161 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc     2221 cacacaacat acgagccgga aghcataaag tgtaaagcct ggggtgccta atgagtgagc     2281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc     2341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct     2401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     2521 atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga     2581 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     2641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     2701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     2761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     2821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     2881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     2941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     3001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     3061 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     3121 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     3181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     3241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     3301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     3361 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg     3421 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa     3481 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc     3541 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg     3601 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat     3661 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca     3721 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga     3781 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg     3841 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg     3901 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata     3961 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca     4021 tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg     4081 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat     4141 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt     4201 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt     4261 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca     4321 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt     4381 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc     4441 tacaacaa