Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin

内毒素:Less than 0.1 EU per μg by the LAL method.

纯度:>95% as determined by

SDS-PAGE:
.

活性:Measured by its binding ability in a functional ELISA. Immobilized Human CD155, Fc Tag (Catalog # CD5-H5251) at 5μg/mL (100 μL/well) can bind Human TIGIT, mouse IgG2a Fc tag (Catalog # TIT-H5253) with a linear range of 3.9-62.5 ng/mL.

SDS-PAGE:
:

产品背景:TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells.

产品描述:This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa on a

SDS-PAGE:
gel under reducing (R) condition due to glycosylation.