ActiveMax® Recombinant Human IL-12

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
The bio-activity was determined by the dose-dependent release of IFN-gamma from the human NK92 cell line in presence of 20 ng/mL rhIL-2 (Catalog # IL2-H4216). The < 0.2 ng/mL, corresponding to a specific activity of >5,000,000 Unit/mg.

产品背景:
Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

产品描述:
ActiveMax® Human IL-12 contains no "tag", and has a calculated MW of 36.6 kDa (IL12B) and 23.8 kDa (IL12A). The predicted N-terminus is Ile 23 (IL12B) and Arg 23 (IL12A). The reducing (R) protein migrates as 33-47 kDa in SDS-PAGE due to glycosylation.