二抗及链霉亲和素_详细资料

产品信息

供应商：伯易生物规格： 500 µg

    免疫荧光技术是将免疫学方法(抗原抗体特异结合)与荧光标记技术结合起来研究特异蛋白抗原在细胞内分布的方法。由于荧光素所发的荧光可在荧光显微镜下检出，从而可对抗原进行细胞定位。

    免疫荧光检测方法根据待检测抗原的丰度采用不同的荧光标记策略。对于高丰度的抗原，可采用荧光标记一抗直接检测的方式；对于中等丰度的抗原，可采用荧光标记二抗的方式对待检测的抗原进行信号放大；对于低等丰度的抗原，可采用生物素标记抗体和荧光标记链霉亲和素的方式对待检测的抗原进行二级信号放大。在整个免疫荧光检测的过程中，荧光染料的性能（信号强度，稳定性，背景信号）对免疫荧光检测的结果发挥着关键性的作用。早期较常用的FITC荧光素标记抗体由于其信号弱，稳定性差，目前正逐渐被新型荧光染料（如Alexa Fluor, Dylight）所取代。
    Applied BioProbes公司ZX推出了一系列性能优良的Andy Fluor荧光染料。这些新型荧光染料跟传统的荧光染料（如FITC）相比，具有无可比拟的优越性。公司新推出了一系列Andy Fluor和生物素标记的二抗及相应的标记服务，为广大科研用户提供了广阔的选择。

产品特点:

荧光信号强；

背景信号低；

光稳定性好；

多色荧光可供选择；

仪器兼容性好；

荧光标记二抗及链霉亲和素选购指南

Secondary Antibody Streptavidin

Andy Fluor™ Dyes Other Fluorescent Labels Biotin & HRP Labels Andy Fluor™ & Cy® Dyes

Andy Fluor™ 350 Cy 3 Biotin Andy Fluor™ 350

Andy Fluor™ 405 Cy 5 HRP Andy Fluor™ 488

Andy Fluor™ 430 Cy 5.5 Andy Fluor™ 555

Andy Fluor™ 488 Cy 7 Andy Fluor™ 594

Andy Fluor™ 555 FITC Andy Fluor™ 647

Andy Fluor™ 568 Cy®3

Andy Fluor™ 594 Cy®5

Andy Fluor™ 647

Andy Fluor™ 680

Andy Fluor™ 750

Andy Fluor™ dye conjugates have brighter fluorescence than other fluorophores.

Figure 1. Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, Cy®3, and Cy®5.

Figure 2. Flow cytometry comparison of the brightness of goat anti-mouse IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, DyLight™ 488, Cy®3, DyLight™ 550, and DyLight™ 650.

Andy Fluor™ dye conjugates have better photostability than other fluorophores.

Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG (H+L) (L109B) with FITC goat anti-mouse IgG (H+L) (L146B). The cytoskeleton of HeLa cells was labeled with mouse monoclonal anti-α-tubulin antibody in combination with Andy Fluor™ 488 goat anti-mouse IgG (H+L) antibody (top series) or with mouse monoclonal anti-α-tubulin antibody in combination with FITC goat anti-mouse IgG (H+L) antibody (bottom series). The fluorescence imaging was taken at 60-second intervals (0, 60, and 120 seconds of exposure).

Figure 4. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, and then visualized with goat anti-mouse IgG antibodies conjugated with either Andy Fluor™ 488 (top, left), Andy Fluor™ 555 (top, right), Andy Fluor™ 568 (bottom, left), or Andy Fluor™ 647 (bottom, right). Nuclei are counterstained with DAPI (blue).

Figure 5. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with green-fluorescent Andy Fluor™ 488 goat anti-rabbit IgG antibody (green). Nuclei were counterstained with DAPI (blue).

Figure 6. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with either Andy Fluor™ 555 (red, left), or Andy Fluor™ 568 (red, right). Nuclei were counterstained with DAPI (blue).

Figure 7. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells and CCSP in mouse lung tissue. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, left). FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, middle and right). Nuclei were counterstained with DAPI (blue).

温馨提示：不可用于临床ZL。

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Secondary Antibody			Streptavidin
Andy Fluor™ Dyes	Other Fluorescent Labels	Biotin & HRP Labels	Andy Fluor™ & Cy® Dyes
Andy Fluor™ 350	Cy 3	Biotin	Andy Fluor™ 350
Andy Fluor™ 405	Cy 5	HRP	Andy Fluor™ 488
Andy Fluor™ 430	Cy 5.5		Andy Fluor™ 555
Andy Fluor™ 488	Cy 7		Andy Fluor™ 594
Andy Fluor™ 555	FITC		Andy Fluor™ 647
Andy Fluor™ 568			Cy®3
Andy Fluor™ 594			Cy®5
Andy Fluor™ 647
Andy Fluor™ 680
Andy Fluor™ 750